LMP-2A (NC_007605) Virus Tagged ORF Clone

CAT#: VC101093

Myc-DDK-tagged ORF clone of viral ORF for K15 [Human herpesvirus 4], codon optimized for human cell expression, YP_401631

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LMP-2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101093 represents NCBI reference of YP_401631 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCACTGGAGATGGTTCCTATGGGCGCTGGTCCCCCCAGCCCGGGCGGAGACCCGGATGGCTATG
  ACGGCGGCAATAACTCCCAGTATCCCTCCGCATCCGGCAGTAGCGGAAACACACCAACCCCCCCAAATGA
  CGAAGAGAGGGAGTCCAATGAAGAGCCCCCCCCTCCTTACGAAGATCCCTACTGGGGCAACGGCGATAGA
  CACTCTGATTACCAACCTCTTGGCACTCAGGATCAGAGCCTGTACTTGGGACTGCAGCACGATGGTAACG
  ATGGGTTGCCCCCACCTCCGTACAGCCCCAGGGACGATAGTAGTCAGCATATTTATGAAGAAGCTGGTCG
  CGGGTCTATGAACCCTGTTTGTCTTCCTGTGATAGTAGCACCTTACCTTTTCTGGCTGGCTGCTATCGCA
  GCCTCCTGTTTCACAGCCTCTGTGAGCACAGTCGTTACTGCCACTGGTCTTGCACTGAGTCTCTTGCTGC
  TGGCTGCTGTTGCGAGTAGCTATGCCGCGGCTCAGAGAAAGCTGCTTACCCCTGTGACGGTGTTGACCGC
  CGTAGTCACATTCTTCGCCATCTGCCTCACCTGGCGCATTGAGGATCCGCCCTTCAATTCTTTGCTCTTT
  GCACTTCTCGCTGCAGCAGGTGGGCTGCAAGGAATTTATGTGCTTGTGATGCTCGTTCTGTTGATACTGG
  CATACAGGCGGCGATGGAGGAGGCTCACTGTGTGTGGTGGCATTATGTTTCTGGCATGCGTTCTTGTTCT
  GATCGTCGATGCCGTGCTGCAGCTGTCACCACTCCTGGGTGCCGTCACCGTGGTCTCCATGACCCTCCTT
  CTTCTGGCATTTGTATTGTGGCTCTCATCCCCTGGAGGATTGGGAACTCTGGGCGCCGCATTGCTGACCC
  TGGCGGCCGCACTGGCCCTGCTCGCTTCTCTGATCCTTGGTACTCTGAACTTGACCACTATGTTTCTCCT
  TATGCTCCTGTGGACCCTGGTCGTACTCCTGATCTGTAGTAGTTGTAGTTCTTGTCCCCTGTCTAAAATC
  CTCCTCGCACGGCTGTTCCTGTACGCCCTGGCACTGCTCCTGCTCGCCTCTGCACTGATCGCGGGTGGCA
  GTATCCTGCAAACAAATTTCAAGAGCCTCTCCAGTACTGAGTTCATCCCAAACCTCTTTTGCATGCTTCT
  GCTCATCGTGGCAGGTATACTGTTCATCCTGGCAATACTTACAGAGTGGGGCAGTGGCAACCGCACTTAC
  GGCCCTGTATTTATGTGTCTCGGAGGGCTGCTTACTATGGTGGCCGGTGCAGTTTGGTTGACAGTTATGT
  CAAATACCCTGCTGAGTGCCTGGATTCTGACGGCGGGTTTTCTCATTTTTCTGATTGGCTTCGCCCTTTT
  CGGCGTGATCAGATGCTGTAGGTACTGCTGCTATTATTGTTTGACCCTCGAATCCGAGGAGAGGCCTCCA
  ACGCCTTACAGGAACACAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101093 representing YP_401631
  Red=Cloning sites Green=Tags
  
  MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNTPTPPNDEERESNEEPPPPYEDPYWGNGDR
  HSDYQPLGTQDQSLYLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLPVIVAPYLFWLAAIA
  ASCFTASVSTVVTATGLALSLLLLAAVASSYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLF
  ALLAAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVLVLIVDAVLQLSPLLGAVTVVSMTLL
  LLAFVLWLSSPGGLGTLGAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICSSCSSCPLSKI
  LLARLFLYALALLLLASALIAGGSILQTNFKSLSSTEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTY
  GPVFMCLGGLLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRCCRYCCYYCLTLESEERPP
  TPYRNTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1491 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401631
• RefSeq ORF: 1491 bp
• Locus ID: 3783751
• UniProt ID: P03211
• MW: 53.0 kDa