BDLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101156

Myc-DDK-tagged ORF clone of viral ORF for BDLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401696

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BDLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101156 represents NCBI reference of YP_401696 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCAAGGTCGTGGTCAGCCTTAGTTCCCGGCTGTATACAGACGAAATTGCAAAAATGCAGCAGA
  GAATCGGTTGCATCCTGCCACTTGCGTCTACCCACGGGACACAGAATGTACAAGGGCTTGGGCTTGGCCA
  GGTCTACAGTCTCGAAACTGTCCCCGACTACGTGAGTATGTATAATTACCTGTCCGACTGCACTCTCGCC
  GTCTTGGATGAAGTTAGCGTTGACTCACTGATCCTCACCAAGATTGTGCCTGGACAGACCTACGCTATAA
  AAAATAAGTACCAGCCATTCTTTCAATGGCACGGCACAGGGTCCTTGAGCGTGATGCCGCCAGTTTTCGG
  TCGGGAACATGCCACAGTTAAACTGGAGAGCAACGACGTCGATATAGTATTTCCCATGGTGCTGCCCACA
  CCTATCGCTGAAGAGGTCCTTCAAAAGATCCTGCTGTTTAATGTATATAGTAGGGTTGTCATGCAAGCCC
  CTGGGAACGCGGATATGCTTGACGTACACATGCACCTGGGGTCAGTGTCATACCTTGGTCATCATTATGA
  GCTGGCACTGCCCGAGGTGCCAGGTCCGCTTGGGCTGGCATTGCTGGATAATCTCTCTCTCTATTTCTGC
  ATCATGGTCACATTGCTTCCGCGCGCCTCTATGAGGCTGGTTCGCGGTCTGATTCGCCACGAGCACCATG
  ACCTTCTGAACCTTTTCCAGGAGATGGTTCCTGATGAGATCGCGAGGATTGATTTGGACGACCTGAGCGT
  GGCCGACGATTTGTCTCGAATGAGGGTCATGATGACCTACCTGCAGAGCCTGGCTTCCCTGTTTAACCTG
  GGTCCCAGGCTGGCTACAGCAGCGTACTCTCAGGAAACCCTGACTGCTACTTGCTGGCTTAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101156 representing YP_401696
  Red=Cloning sites Green=Tags
  
  MDLKVVVSLSSRLYTDEIAKMQQRIGCILPLASTHGTQNVQGLGLGQVYSLETVPDYVSMYNYLSDCTLA
  VLDEVSVDSLILTKIVPGQTYAIKNKYQPFFQWHGTGSLSVMPPVFGREHATVKLESNDVDIVFPMVLPT
  PIAEEVLQKILLFNVYSRVVMQAPGNADMLDVHMHLGSVSYLGHHYELALPEVPGPLGLALLDNLSLYFC
  IMVTLLPRASMRLVRGLIRHEHHDLLNLFQEMVPDEIARIDLDDLSVADDLSRMRVMMTYLQSLASLFNL
  GPRLATAAYSQETLTATCWLR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 903 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401696
• RefSeq ORF: 903 bp
• Locus ID: 3783692
• UniProt ID: P03211
• MW: 33.6 kDa