BBRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101142

Myc-DDK-tagged ORF clone of viral ORF for BBRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401682

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101142 represents NCBI reference of YP_401682 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACATGAATGTGGATGAAAGCGCCTCAGGTGCCCTGGGCAGCAGTGCCATCCCAGTTCACCCCA
  CGCCCGCATCAGTGCGCCTGTTCGAGATCCTTCAGGGGAAGTACGCATATGTGCAGGGGCAGACTATCTA
  TGCTAATTTGCGGAACCCGGGCGTGTTCAGTAGGCAGGTGTTTACTCACCTTTTTAAACGCGCCATTTCA
  CACTGTACATACGACGACGTACTTCACGACTGGAACAAATTTGAGGCCTGCATCCAAAAGAGATGGCCAA
  GTGACGACTCATGTGCCAGCAGATTCAGGGAGTCAACATTTGAATCCTGGAGTACCACCATGAAGCTTAC
  CGTCAGGGACCTGTTGACCACCAACATCTACAGGGTCTTGCACAGCAGATCCGTGCTCTCCTATGAACGA
  TACGTTGACTGGATCTGTGCAACCGGTATGGTGCCTGCCGTCAAGAAACCTATCACACAGGAGCTGCACA
  GCAAGATTAAGTCTCTGAGAGATCGCTGCGTGTGTAGAGAATTGGGACATGAGCGCACTATCAGGTCTAT
  CGGGACTGAGCTGTACGAGGCCACAAAGGAAATAATCGAGTCTCTGAATTCCACGTTTATTCCCCAGTTC
  ACAGAAGTAACTATTGAATACCTGCCACGATCCGATGAGTACGTTGCATACTACTGTGGCCGCCGCATAA
  GGCTGCATGTGTTGTTCCCTCCTGCAATTTTCGCAGGAACCGTCACTTTCGATTCTCCCGTGCAGAGACT
  GTATCAAAATATCTTTATGTGCTATCGGACCCTTGAGCACGCCAAGATCTGTCAGCTGTTGAACACCGCT
  CCCCTTAAGGCGATCGTGGGTCACGGAGGCCGCGACATGTATAAGGATATCCTCGCTCACCTCGAGCAGA
  ATAGCCAGCGGAAAGATCCCAAGAAGGAGCTGCTGAATTTGCTTGTTAAACTTAGCGAAAATAAGACGAT
  CAGCGGAGTGACAGACGTTGTTGAGGAGTTTATCACGGATGCGAGTAACAATCTCGTTGATCGGAATAGA
  CTGTTTGGTCAACCCGGCGAAACTGCTGCCCAAGGGCTCAAGAAAAAAGTGTCCAACACAGTTGTGAAGT
  GTCTGACTGACCAGATAAATGAGCAGTTCGACCAAATTAACGGCCTGGAGAAGGAACGGGAGCTTTATTT
  GAAGAAGATCAGATCCATGGAATCCCAACTGCAGGCGTCTCTGGGGCCGGGCGGCAACAATCCAGCGGCC
  TCAGCTCCCGCCGCCGTCGCAGCTGAAGCCGCATCTGTTGATATCCTGACAGGCAGCACCGCCTCAGCGA
  TCGAGAAACTGTTTAATTCCCCAAGTGCCTCTCTGGGGGCAAGGGTTTCTGGACACAATGAATCCATCCT
  GAACTCATTTGTCAGCCAATATATCCCACCCTCCAGAGAAATGACCAAAGACCTGACCGAGCTTTGGGAG
  AGCGAGTTGTTCAACACCTTCAAGCTTACCCCTGTGGTGGATAACCAGGGCCAGCGCCTCTATGTGCGCT
  ACAGTTCCGATACAATCAGCATTCTCCTCGGCCCATTCACGTACCTGGTGGCTGAGCTGTCTCCAGTCGA
  GCTGGTAACAGACGTATATGCCACTCTGGGAATTGTCGAAATCATTGACGAGCTCTACAGGTCAAGCCGG
  CTGGCAATCTATATCGAAGATCTTGGTAGAAAATACTGCCCAGCAAGCGCTACCGGCGGCGATCATGGGA
  TCCGACAAGCCCCTTCCGCACGAGGGGACACAGAGCCTGATCATGCCAAATCAAAGCCTGCTCGAGATCC
  ACCACCAGGGGCAGGTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101142 representing YP_401682
  Red=Cloning sites Green=Tags
  
  MFNMNVDESASGALGSSAIPVHPTPASVRLFEILQGKYAYVQGQTIYANLRNPGVFSRQVFTHLFKRAIS
  HCTYDDVLHDWNKFEACIQKRWPSDDSCASRFRESTFESWSTTMKLTVRDLLTTNIYRVLHSRSVLSYER
  YVDWICATGMVPAVKKPITQELHSKIKSLRDRCVCRELGHERTIRSIGTELYEATKEIIESLNSTFIPQF
  TEVTIEYLPRSDEYVAYYCGRRIRLHVLFPPAIFAGTVTFDSPVQRLYQNIFMCYRTLEHAKICQLLNTA
  PLKAIVGHGGRDMYKDILAHLEQNSQRKDPKKELLNLLVKLSENKTISGVTDVVEEFITDASNNLVDRNR
  LFGQPGETAAQGLKKKVSNTVVKCLTDQINEQFDQINGLEKERELYLKKIRSMESQLQASLGPGGNNPAA
  SAPAAVAAEAASVDILTGSTASAIEKLFNSPSASLGARVSGHNESILNSFVSQYIPPSREMTKDLTELWE
  SELFNTFKLTPVVDNQGQRLYVRYSSDTISILLGPFTYLVAELSPVELVTDVYATLGIVEIIDELYRSSR
  LAIYIEDLGRKYCPASATGGDHGIRQAPSARGDTEPDHAKSKPARDPPPGAGS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1839 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401682
• RefSeq ORF: 1839 bp
• Locus ID: 3783764
• UniProt ID: P03211
• MW: 68.5 kDa