BRLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101134

Myc-DDK-tagged ORF clone of viral ORF for BRLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401674

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101134 represents NCBI reference of YP_401674 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCAAAAAAAGACGGTTTGGAGGATTTCCTTCGACTCACTCCTGAGATCAAGAAACAGCTGGGAT
  CCCTCGTTTCTGACTACTGTAATGTTCTGAATAAGGAGTTTACAGCCGGATCTGTGGAGATCACACTGCG
  CTCCTACAAGATCTGCAAGGCCTTCATTAATGAGGCGAAAGCTCACGGTAGGGAGTGGGGTGGTCTCATG
  GCTACTCTCAACATTTGCAACTTTTGGGCCATACTTAGAAATAATCGGGTCAGGAGAAGGGCCGAGAACG
  CCGGTAACGACGCTTGCTCAATAGCCTGTCCCATTGTGATGCGCTACGTGCTTGATCATCTCATTGTGGT
  CACCGACAGATTTTTCATCCAGGCGCCAAGTAATAGGGTCATGATCCCCGCGACTATCGGTACCGCCATG
  TACAAACTCCTCAAACACTCTCGAGTTCGGGCCTATACCTACAGCAAGGTGTTGGGTGTGGACAGGGCCG
  CCATTATGGCGAGCGGAAAGCAAGTTGTTGAGCACCTGAACCGGATGGAAAAGGAAGGCCTCTTGAGCTC
  TAAGTTCAAGGCATTCTGCAAGTGGGTGTTTACCTACCCTGTGCTGGAGGAGATGTTCCAGACCATGGTG
  TCTAGTAAGACTGGACACCTTACAGATGATGTGAAAGACGTTCGAGCGCTCATCAAGACACTTCCACGAG
  CCTCCTATTCCTCACACGCAGGCCAGCGCAGCTACGTCAGTGGAGTCCTCCCGGCATGTCTGCTTTCAAC
  AAAATCTAAGGCTGTGGAGACACCTATCCTGGTTTCTGGCGCAGACCGCATGGACGAGGAACTTATGGGA
  AATGATGGGGGCGCATCTCACACAGAGGCCCGGTACTCAGAAAGCGGACAATTTCATGCTTTTACCGACG
  AACTGGAGAGTCTGCCATCCCCTACAATGCCCTTGAAACCCGGGGCTCAGAGCGCCGATTGTGGCGATTC
  AAGCAGCTCCTCATCCGACAGTGGCAATAGTGATACAGAGCAAAGTGAGCGGGAGGAGGCCAGAGCCGAG
  GCCCCTCGATTGAGAGCACCAAAATCTCGGCGCACGAGTAGGCCTAATCGGGGCCAAACCCCCTGTCCGT
  CAAACGCCGCAGAGCCAGAGCAGCCCTGGATCGCCGCTGTGCACCAGGAAAGTGACGAGCGCCCAATTTT
  CCCTCATCCGAGCAAGCCTACATTCCTGCCTCCCGTTAAACGCAAGAAGGGATTGAGAGATTCTAGGGAG
  GGCATGTTTCTGCCAAAACCAGAGGCAGGCAGTGCAATCTCCGACGTGTTTGAGGGGCGGGAAGTTTGTC
  AGCCTAAGCGGATACGACCATTCCATCCCCCTGGAAGCCCTTGGGCCAATAGACCCCTGCCCGCCTCTTT
  GGCCCCCACACCCACCGGCCCTGTCCATGAGCCTGTCGGAAGTTTGACACCGGCTCCCGTACCACAGCCT
  CTTGATCCCGCCCCTGCAGTGACCCCCGAGGCAAGCCATCTCCTGGAAGATCCGGACGAGGAAACTTCAC
  AAGCGGTCAAGGCTCTGCGGGAGATGGCAGATACCGTGATCCCCCAAAAAGAGGAAGCCGCAATTTGTGG
  ACAAATGGATCTGAGCCATCCCCCGCCCAGAGGGCACCTTGACGAGCTGACCACGACACTGGAAAGCATG
  ACTGAAGACCTGAATCTCGACTCCCCCTTGACCCCTGAGCTCAACGAGATACTCGATACATTCCTTAATG
  ATGAGTGCCTCTTGCATGCTATGCACATAAGCACCGGCTTGTCTATCTTTGACACAAGTCTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101134 representing YP_401674
  Red=Cloning sites Green=Tags
  
  MRPKKDGLEDFLRLTPEIKKQLGSLVSDYCNVLNKEFTAGSVEITLRSYKICKAFINEAKAHGREWGGLM
  ATLNICNFWAILRNNRVRRRAENAGNDACSIACPIVMRYVLDHLIVVTDRFFIQAPSNRVMIPATIGTAM
  YKLLKHSRVRAYTYSKVLGVDRAAIMASGKQVVEHLNRMEKEGLLSSKFKAFCKWVFTYPVLEEMFQTMV
  SSKTGHLTDDVKDVRALIKTLPRASYSSHAGQRSYVSGVLPACLLSTKSKAVETPILVSGADRMDEELMG
  NDGGASHTEARYSESGQFHAFTDELESLPSPTMPLKPGAQSADCGDSSSSSSDSGNSDTEQSEREEARAE
  APRLRAPKSRRTSRPNRGQTPCPSNAAEPEQPWIAAVHQESDERPIFPHPSKPTFLPPVKRKKGLRDSRE
  GMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWANRPLPASLAPTPTGPVHEPVGSLTPAPVPQP
  LDPAPAVTPEASHLLEDPDEETSQAVKALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESM
  TEDLNLDSPLTPELNEILDTFLNDECLLHAMHISTGLSIFDTSLF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401674
• RefSeq ORF: 1815 bp
• Locus ID: 3783727
• UniProt ID: P03211
• MW: 66.6 kDa