BORF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101117

Myc-DDK-tagged ORF clone of viral ORF for BORF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401655

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BORF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101117 represents NCBI reference of YP_401655 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCACCAGTCACGTGGAGCATGAATTGCTGAGCAAGCTGATCGACGAACTGAAGGTCAAGGCCA
  ACAGCGATCCAGAAGCCGACGTCCTGGCGGGCAGGTTGCTGCATAGGTTGAAGGCAGAATCTGTGACGCA
  CACTGTTGCCGAGTATCTGGAAGTGTTTAGCGACAAATTCTACGATGAAGAGTTCTTTCAAATGCACCGG
  GATGAGCTGGAGACGAGGGTATCAGCCTTTGCCCAGTCTCCAGCCTATGAAAGGATCGTAAGCTCAGGAT
  ACCTGTCCGCACTCCGCTACTACGACACCTATCTTTATGTAGGACGGAGCGGGAAGCAGGAGTCAGTACA
  GCACTTTTATATGCGGCTCGCTGGCTTCTGTGCTTCAACCACATGCTTGTATGCTGGGCTTCGCGCCGCC
  CTGCAGAGGGCTCGGCCTGAGATAGAGTCAGATATGGAAGTCTTTGACTATTACTTTGAACACTTGACTT
  CTCAGACAGTCTGTTGCAGTACCCCGTTTATGCGCTTTGCTGGGGTGGAAAATTCAACACTCGCTTCTTG
  TATTCTCACCACCCCCGACCTCTCTAGCGAATGGGATGTTACACAGGCCTTGTATAGACACCTGGGGCGG
  TATCTCTTTCAAAGAGCCGGCGTTGGTGTCGGGGTGACCGGCGCCGGACAGGATGGAAAACATATAAGCC
  TTCTCATGCGAATGATTAACTCCCATGTGGAATATCATAACTACGGTTGCAAAAGGCCTGTCAGCGTTGC
  CGCCTATATGGAACCCTGGCACTCCCAGATTTTTAAGTTCCTCGAGACCAAATTGCCAGAGAATCATGAG
  CGATGCCCGGGGATATTTACCGGGCTCTTCGTCCCCGAACTTTTCTTCAAGCTCTTCCGGGACACACCCT
  GGTCTGACTGGTACCTGTTTGATCCCAAGGACGCCGGAGACCTGGAAAGGCTCTACGGCGAGGAGTTTGA
  GAGGGAATACTATCGCCTGGTTACTGCCGGCAAGTTCTGCGGACGGGTCAGTATTAAATCACTCATGTTC
  TCAATAGTCAATTGTGCAGTTAAGGCTGGCAGCCCCTTCATACTCCTGAAGGAAGCATGCAACGCACATT
  TTTGGAGAGATTTGCAGGGTGAAGCAATGAATGCTGCCAACCTTTGTGCCGAGGTGCTCCAACCTTCCCG
  CAAGAGTGTCGCGACCTGCAACTTGGCGAATATTTGTTTGCCGCGATGTCTGGTGAACGCACCTCTCGCA
  GTGCGAGCCCAGAGAGCAGACACCCAGGGAGATGAGCTCTTGCTTGCCCTGCCCCGGCTCTCTGTCACAT
  TGCCTGGCGAAGGGGCAGTGGGGGATGGTTTCTCACTGGCACGACTGCGGGACGCTACACAGTGCGCAAC
  CTTCGTGGTGGCCTGTTCCATCTTGCAGGGAAGCCCTACATACGACTCAAGAGATATGGCGTCCATGGGA
  CTGGGCGTGCAGGGGTTGGCCGACGTTTTCGCTGACTTGGGATGGCAGTACACAGACCCTCCTTCCAGAT
  CCCTCAACAAGGAGATTTTCGAACATATGTATTTTACGGCTCTGTGCACCTCCTCCCTCATTGGTCTTCA
  CACCCGAAAGATTTTCCCAGGATTCAAGCAGTCAAAATATGCAGGAGGATGGTTCCACTGGCATGATTGG
  GCGGGTACTGATCTTTCTATCCCACGCGAAATTTGGTCCAGACTGTCCGAGCGGATCGTGAGGGATGGGC
  TCTTCAATAGCCAGTTCATCGCGCTGATGCCCACCTCAGGTTGTGCACAGGTAACGGGTTGTTCCGATGC
  CTTCTATCCATTTTATGCCAACGCAAGCACCAAGGTCACCAATAAGGAGGAGGCACTGCGGCCAAACCGG
  AGCTTTTGGAGACATGTTCGACTCGATGATCGCGAGGCCTTGAACCTTGTAGGCGGGAGAGTGTCCTGTC
  TCCCTGAAGCACTCCGGCAGAGGTATCTCCGATTCCAAACTGCATTTGACTACAACCAGGAAGATTTGAT
  CCAGATGTCTAGGGACCGCGCACCGTTCGTCGACCAGAGCCAGAGCCATTCCCTTTTTCTCCGGGAAGAA
  GATGCTGCACGAGCTAGCACCCTCGCCAACCTCCTGGTCCGCTCTTATGAACTTGGGCTGAAAACAATTA
  TGTACTACTGCCGCATTGAGAAAGCTGCAGATCTTGGCGTCATGGAATGTAAAGCGAGTGCCGCCCTCTC
  AGTGCCCCGCGAAGAACAGAATGAGCGGTCTCCCGCAGAGCAGATGCCACCAAGGCCGATGGAACCTGCC
  CAAGTGGCTGGGCCGGTCGATATCATGAGCAAAGGGCCTGGTGAGGGACCAGGGGGGTGGTGCGTGCCAG
  GAGGGCTGGAGGTCTGCTACAAGTACCGGCAGCTGTTCAGCGAGGATGATTTGCTCGAAACCGATGGCTT
  TACAGAGAGGGCGTGCGAGTCTTGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101117 representing YP_401655
  Red=Cloning sites Green=Tags
  
  MATTSHVEHELLSKLIDELKVKANSDPEADVLAGRLLHRLKAESVTHTVAEYLEVFSDKFYDEEFFQMHR
  DELETRVSAFAQSPAYERIVSSGYLSALRYYDTYLYVGRSGKQESVQHFYMRLAGFCASTTCLYAGLRAA
  LQRARPEIESDMEVFDYYFEHLTSQTVCCSTPFMRFAGVENSTLASCILTTPDLSSEWDVTQALYRHLGR
  YLFQRAGVGVGVTGAGQDGKHISLLMRMINSHVEYHNYGCKRPVSVAAYMEPWHSQIFKFLETKLPENHE
  RCPGIFTGLFVPELFFKLFRDTPWSDWYLFDPKDAGDLERLYGEEFEREYYRLVTAGKFCGRVSIKSLMF
  SIVNCAVKAGSPFILLKEACNAHFWRDLQGEAMNAANLCAEVLQPSRKSVATCNLANICLPRCLVNAPLA
  VRAQRADTQGDELLLALPRLSVTLPGEGAVGDGFSLARLRDATQCATFVVACSILQGSPTYDSRDMASMG
  LGVQGLADVFADLGWQYTDPPSRSLNKEIFEHMYFTALCTSSLIGLHTRKIFPGFKQSKYAGGWFHWHDW
  AGTDLSIPREIWSRLSERIVRDGLFNSQFIALMPTSGCAQVTGCSDAFYPFYANASTKVTNKEEALRPNR
  SFWRHVRLDDREALNLVGGRVSCLPEALRQRYLRFQTAFDYNQEDLIQMSRDRAPFVDQSQSHSLFLREE
  DAARASTLANLLVRSYELGLKTIMYYCRIEKAADLGVMECKASAALSVPREEQNERSPAEQMPPRPMEPA
  QVAGPVDIMSKGPGEGPGGWCVPGGLEVCYKYRQLFSEDDLLETDGFTERACESCQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401655
• RefSeq ORF: 2478 bp
• Locus ID: 3783725
• UniProt ID: P03211
• MW: 93.0 kDa