BFLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101110

Myc-DDK-tagged ORF clone of viral ORF for BFLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401648

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BFLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101110 represents NCBI reference of YP_401648 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACAAGGTGACCAGCGCCAATGAGCCCAACCCACTGACCGGCAAGCGGCTGAGCAGCTGTCCCC
  TGACCAGAAGCGGCGTGACCGAGGTGGCACAAATTGCCGGACGGACCCCCAAGATGGAGGACTTCGTGCC
  ATGGACCGTCGACAACCTGAAGAGCCAGTTTGAGGCTGTGGGCCTGCTCATGGCTCACTCCTACCTGCCT
  GCCAACGCCGAGGAGGGCATCGCCTACCCTCCCCTGGTGCACACCTACGAGAGCCTGTCCCCCGCCAGCA
  CCTGCAGAGTCTGCGATCTGCTCGACACCCTCGTGAACCACAGCGACGCCCCAGTGGCCTTCTTCGAAGA
  CTACGCACTGCTGTGCTACTACTGCCTCAACGCCCCCCGGGCTTGGATCAGCAGCCTGATTACCGGGATG
  GATTTCCTGCACATCCTGATCAAGTACTTCCCCATGGCCGGCGGCCTGGACTCCCTGTTCATGCCCAGCC
  GGATCCTGGCCATCGACATCCAGCTGCACTTCTACATCTGCCGGTGCTTCCTGCCCGTGAGCAGCAGCGA
  CATGATCCGGAACGCCAACCTGGGGTACTACAAGCTCGAGTTCCTGAAGAGCATCCTCACCGGCCAGAGC
  CCCGCCAACTTTTGCTTCAAGAGCATGTGGCCCCGGACCACCCCCACTTTTCTCACCCTGCCAGGGCCCC
  GGACCTGCAAGGACAGCCAGGACGTGCCCGGCGACGTGGGCCGGGGCCTCTACACTGCCCTCTGCTGCCA
  CCTGCCCACCCGGAACCGGGTCCAGCACCCCTTCCTGCGGGCCGAGAAGGGCGGGCTCTCCCCCGAAATC
  ACCACCAAAGCCGACTACTGCGGCCTGCTGCTCGGCACCTGGCAGGGCACCGACCTGCTGGGCGGACCCG
  GCCACCACGCCATCGGCCTCAATGCCGAATACAGCGGCGACGAACTGGCCGAACTCGCCCTCGCCATCAC
  CCGGCCCGAGGCCGGCGACCACAGCCAGGGGCCCTGCCTGCTGGCACCCATGTTCGGCCTCCGGCACAAG
  AACGCCAGCCGGACCATCTGCCCTCTCTGCGAGTCCCTCGGCGCCCACCCCGACGCCAAGGACACACTGG
  ACAGATTCAAGTCCCTCATCCTGGACAGCTTCGGCAACAACATCAAGATCCTCGACCGGATCGTGTTCCT
  CATTAAGACCCAGAACACCCTGCTGGACGTCCCCTGCCCCCGGCTGCGGGCCTGGCTGCAAATGTGTACC
  CCCCAGGATTTCCACAAGCACCTGTTTTGTGACCCCCTGTGCGCCATCAACCATAGCATCACCAACCCAA
  GCGTGCTGTTTGGCCAGATCTACCCCCCCAGCTTCCAGGCCTTCAAGGCCGCCCTCGCCGCAGGCCAGAA
  CCTCGAGCAGGGCGTGTGCGACAGCCTGATCACCCTCGTCTACATTTTCAAAAGCACCCAGGTGGCTCGG
  GTCGGCAAAACCATCCTCGTGGACGTCACAAAGGAGCTGGATGTCGTGCTGAGAATCCACGGCCTGGATC
  TCGTGCAGTCCTACCAGACAAGCCAAGTGTATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101110 representing YP_401648
  Red=Cloning sites Green=Tags
  
  MAHKVTSANEPNPLTGKRLSSCPLTRSGVTEVAQIAGRTPKMEDFVPWTVDNLKSQFEAVGLLMAHSYLP
  ANAEEGIAYPPLVHTYESLSPASTCRVCDLLDTLVNHSDAPVAFFEDYALLCYYCLNAPRAWISSLITGM
  DFLHILIKYFPMAGGLDSLFMPSRILAIDIQLHFYICRCFLPVSSSDMIRNANLGYYKLEFLKSILTGQS
  PANFCFKSMWPRTTPTFLTLPGPRTCKDSQDVPGDVGRGLYTALCCHLPTRNRVQHPFLRAEKGGLSPEI
  TTKADYCGLLLGTWQGTDLLGGPGHHAIGLNAEYSGDELAELALAITRPEAGDHSQGPCLLAPMFGLRHK
  NASRTICPLCESLGAHPDAKDTLDRFKSLILDSFGNNIKILDRIVFLIKTQNTLLDVPCPRLRAWLQMCT
  PQDFHKHLFCDPLCAINHSITNPSVLFGQIYPPSFQAFKAALAAGQNLEQGVCDSLITLVYIFKSTQVAR
  VGKTILVDVTKELDVVLRIHGLDLVQSYQTSQVYV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401648
• RefSeq ORF: 1575 bp
• Locus ID: 3783697
• UniProt ID: P03211
• MW: 57.9 kDa