Snf1lk2 (SIK2) (NM_015191) Human Tagged ORF Clone

CAT#: RC221327

SIK2 (Myc-DDK-tagged)-Human salt-inducible kinase 2 (SIK2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402414) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221327 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SIK2 protein (Cat# TP321327). The protein was produced from HEK293T cells transfected with SIK2 cDNA clone (Cat# RC221327) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIK2
• Synonyms: LOH11CR1I; QIK; SIK-2; SNF1LK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221327 representing NM_015191
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGTGGGGTTCTACGACATCGAGG
  GCACGCTGGGCAAGGGCAACTTCGCTGTGGTGAAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGC
  AATAAAAATAATCGATAAGTCTCAGCTGGATGCAGTGAACCTTGAGAAAATCTACCGAGAAGTACAAATA
  ATGAAAATGTTAGACCACCCTCACATAATCAAACTTTATCAGGTAATGGAGACCAAAAGTATGTTGTACC
  TTGTGACAGAATATGCCAAAAATGGAGAAATTTTTGACTATCTTGCTAATCATGGCCGGTTAAATGAGTC
  TGAAGCCAGGCGAAAATTCTGGCAAATCCTGTCTGCTGTTGATTATTGTCATGGTCGGAAGATTGTGCAC
  CGTGACCTCAAAGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGATTTCGGTTTTG
  GAAATTTCTTTAAAAGTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCCCTTATGCAGCCCCAGAAGT
  CTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGACATCTGGAGTATGGGAGTTGTTCTTTATGTCCTT
  GTCTGTGGAGCTCTGCCCTTTGATGGACCGACTCTTCCAATTTTGAGGCAGAGGGTTCTGGAAGGAAGAT
  TCCGGATTCCGTATTTCATGTCAGAAGATTGCGAGCACCTTATCCGAAGGATGTTGGTCCTAGACCCATC
  CAAACGGCTAACCATAGCCCAAATCAAGGAGCATAAATGGATGCTCATAGAAGTTCCTGTCCAGAGACCT
  GTTCTCTATCCACAAGAGCAAGAAAATGAGCCATCCATCGGGGAGTTTAATGAGCAGGTTCTGCGACTGA
  TGCACAGCCTTGGAATAGATCAGCAGAAAACCATTGAGTCTTTGCAGAACAAGAGCTATAACCACTTTGC
  TGCCATTTATTTCTTGTTGGTGGAGCGCCTGAAATCACATCGGAGCAGTTTCCCAGTGGAGCAGAGACTT
  GATGGCCGCCAGCGTCGGCCTAGCACCATTGCTGAGCAAACAGTTGCCAAGGCACAGACTGTGGGGCTCC
  CAGTGACCATGCATTCACCGAACATGAGGCTGCTGCGATCTGCCCTCCTCCCCCAGGCATCCAACGTGGA
  GGCCTTTTCATTTCCAGCATCTGGCTGTCAGGCGGAAGCTGCATTCATGGAAGAAGAGTGTGTGGACACT
  CCAAAGGTCAATGGCTGTCTGCTTGACCCTGTGCCTCCTGTCCTGGTGCGGAAGGGATGCCAGTCACTGC
  CCAGCAACATGATGGAGACCTCCATTGACGAAGGGCTGGAGACAGAAGGAGAGGCCGAGGAAGACCCCGC
  TCATGCCTTTGAGGCATTTCAGTCCACACGCAGCGGGCAGAGACGGCACACTCTGTCAGAAGTGACCAAT
  CAACTGGTCGTGATGCCTGGGGCAGGGAAAATTTTCTCCATGAATGACAGCCCCTCCCTTGACAGTGTGG
  ACTCTGAGTATGATATGGGGTCTGTTCAGAGGGACCTGAACTTTCTGGAAGACAACCCTTCCCTTAAGGA
  CATCATGTTAGCCAATCAGCCTTCACCCCGCATGACATCTCCCTTCATAAGCCTGAGACCTACCAACCCA
  GCCATGCAGGCTCTGAGCTCCCAGAAACGAGAGGTCCACAACAGGTCTCCAGTGAGCTTCAGAGAGGGCC
  GCAGAGCATCAGATACCTCCCTCACCCAGGGAATTGTAGCATTTAGACAACATCTTCAGAATCTGGCTAG
  AACCAAAGGAATTCTAGAGTTGAACAAAGTGCAGTTGTTGTATGAACAAATAGGACCGGAGGCAGACCCT
  AACCTGGCGCCGGCGGCTCCTCAGCTCCAGGACCTTGCTAGCAGCTGCCCTCAGGAAGAAGTTTCTCAGC
  AGCAGGAAAGCGTCTCCACTCTCCCTGCCAGCGTGCATCCCCAGCTGTCCCCACGGCAGAGCCTGGAGAC
  CCAGTACCTGCAGCACAGACTCCAGAAGCCCAGCCTTCTGTCAAAGGCCCAGAACACCTGTCAGCTTTAT
  TGCAAAGAACCACCGCGGAGCCTTGAGCAGCAGCTGCAGGAACATAGGCTCCAGCAGAAGCGACTCTTTC
  TTCAGAAGCAGTCTCAACTGCAGGCCTATTTTAATCAGATGCAGATAGCAGAGAGCTCCTACCCACAGCC
  AAGTCAGCAGCTGCCCCTTCCCCGCCAGGAGACTCCACCGCCTTCTCAGCAGGCCCCACCGTTCAGCCTG
  ACCCAGCCCCTGAGCCCCGTCCTGGAGCCTTCCTCCGAGCAGATGCAATACAGCCCTTTCCTCAGCCAGT
  ACCAAGAGATGCAGCTTCAGCCCCTGCCCTCCACTTCCGGTCCCCGGGCTGCTCCTCCTCTGCCCACGCA
  GCTACAGCAGCAGCAGCCGCCACCGCCACCACCCCCTCCACCACCACGACAGCCAGGAGCTGCCCCAGCC
  CCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCGCTGCTTCCCCTGCGCCAGACTATCCCACTC
  CCTGTCAGTATCCTGTGGATGGAGCCCAGCAGAGCGACCTAACGGGGCCAGACTGTCCCAGAAGCCCAGG
  ACTGCAAGAGGCCCCCTCCAGCTACGACCCACTAGCCCTCTCTGAGCTACCTGGACTCTTTGATTGTGAA
  ATGCTAGACGCTGTGGATCCACAACACAACGGGTATGTCCTGGTGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221327 representing NM_015191
  Red=Cloning site Green=Tags(s)
  
  MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQI
  MKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVH
  RDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVL
  VCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRP
  VLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRL
  DGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDT
  PKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTN
  QLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNP
  AMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADP
  NLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLY
  CKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSL
  TQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPA
  PLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCE
  MLDAVDPQHNGYVLVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015191
• ORF Size: 2778 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015191.3
• RefSeq Size: 5694 bp
• RefSeq ORF: 2781 bp
• Locus ID: 23235
• UniProt ID: Q9H0K1
• Cytogenetics: 11q23.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 103.7 kDa
• Gene Summary: Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.[UniProtKB/Swiss-Prot Function]