DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CAT#: TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

Product Images

Immunoblot analysis of 6 different C-terminal Myc/DDK tagged overexpression lysates(Lane 1.CST4;2.LSM1;3.ALDOB;4.ALDH3A1;5.PLK1;6.METTL16) with TA592569 at 1:3000.

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  Immunoblot analysis of picked 6 different DDK tagged overexpression lysates with TA592569 at 1:3000.(Lane 1/2 N-terminal DDK tag;Lane 3/4 C-terminal 3XDDK tag; Lane 5/6 N-terminal 3XDDK tag.)

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  Immunohistology staining on IL6 overexpressed cytosection TS402078P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC202078, 5 micron sections, 40x magnification

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  Immunohistology staining on TNF overexpressed cytosection TS406983P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC206983, 5 micron sections, 40x magnification

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  Immunohistology staining on IL11 overexpressed cytosection TS404493P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC204493, 5 micron sections, 40x magnification

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  Immunohistology staining on IL10 overexpressed cytosection TS416785P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC216785, 5 micron sections, 40x magnification

• Immunohistology staining on IL1B overexpressed cytosection TS402079P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC202079, 5 micron sections, 40x magnification

• Immunohistology staining on PCDHA11 overexpressed cytosection TS421707P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC221707, 5 micron sections, 40x magnification

• Immunohistology staining on CLDN22 overexpressed cytosection TS425263P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC225263, 5 micron sections, 40x magnification

• Immunohistology staining on CH25H overexpressed cytosection TS404716P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC204716, 5 micron sections, 40x magnification

• Immunohistology staining on SPON2 overexpressed cytosection TS406764P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC206764, 5 micron sections, 40x magnification

• Immunofluorescent staining of 293T cells transfected by human CTAG1B plasmid(RC213318) using anti-DDK antibody (TA592569/green, right), compared to an Isotype control(Left).(1:400)

• Immunoprecipitation (IP) of DDK by using DDK Rabbit monoclonal antibody TA592569. Rabbit IgG control serves as the negative control. 293T cells were transfected with C-terminal DDK-tagged OTUB2 overexpression plasmid. 1ml overexpression cell lysates were first precleared with agarose beads for 1h. Then precleared lysates were incubated with beads crosslinked with antibody for 2h. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with anti-DDK mouse monoclonal antibody(TA180144, 1:1000).

• Immunoprecipitation (IP) of DDK by using DDK Rabbit monoclonal antibody TA592569. Rabbit IgG control serves as the negative control. 293T cells were transfected with N-terminal DDK-tagged Trim29 overexpression plasmid. 1ml overexpression cell lysates were first precleared with agarose beads for 1h. Then precleared lysates were incubated with beads crosslinked with antibody for 2h. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with anti-DDK mouse monoclonal antibody(TA50011, 1:1000).

• Flow cytometric analysis of living 293T cells transfected with human CD37 plasmid (RC210768) (Red) using an anti-DDK antibody (TA592569), compared to an isotype control (green) and a PBS control (blue)(1:100).

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Specifications

Product Data

• Clone Name: OTIR5G2
• Applications: ELISA, FACS, FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:3000, ELISA 1:5000, IP 1:1000
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within DDK. The exact sequence is proprietary.
• Isotype: IgG
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Background: Flag is widely used as an additional tag in expression vectors. The tag can be fused with the target protein at the N-terminal and C-terminal, and can be detected.