Snf1lk2 (SIK2) (NM_015191) Human Untagged Clone

CAT#: SC323400

SIK2 (untagged)-Kinase deficient mutant (K49M) of Human salt-inducible kinase 2 (SIK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Snf1lk2
• Synonyms: LOH11CR1I; QIK; SIK-2; SNF1LK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323400 sequence for NM_015191 edited (data generated by NextGen Sequencing)
  ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGTGGGGTTCTAC
  GACATCGAGGGCACGCTGGGCAAGGGCAACTTCGCTGTGGTGAAGCTGGGGCGGCACCGG
  ATCACCAAGACGGAGGTGGCAATAATGATAATCGATAAGTCTCAGCTGGATGCAGTGAAC
  CTTGAGAAAATCTACCGAGAAGTACAAATAATGAAAATGTTAGACCACCCTCACATAATC
  AAACTTTATCAGGTAATGGAGACCAAAAGTATGTTGTACCTTGTGACAGAATATGCCAAA
  AATGGAGAAATTTTTGACTATCTTGCTAATCATGGCCGGTTAAATGAGTCTGAAGCCAGG
  CGAAAATTCTGGCAAATCCTGTCTGCTGTTGATTATTGTCATGGTCGGAAGATTGTGCAC
  CGTGACCTCAAAGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGAT
  TTCGGTTTTGGAAATTTCTTTAAAAGTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCC
  CCTTATGCAGCCCCAGAAGTCTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGACATC
  TGGAGTATGGGAGTTGTTCTTTATGTCCTTGTCTGTGGAGCTCTGCCCTTTGATGGACCG
  ACTCTTCCAATTTTGAGGCAGAGGGTTCTGGAAGGAAGATTCCGGATTCCGTATTTCATG
  TCAGAAGATTGCGAGCACCTTATCCGAAGGATGTTGGTCCTAGACCCATCCAAACGGCTA
  ACCATAGCCCAAATCAAGGAGCATAAATGGATGCTCATAGAAGTTCCTGTCCAGAGACCT
  GTTCTCTATCCACAAGAGCAAGAAAATGAGCCATCCATCGGGGAGTTTAATGAGCAGGTT
  CTGCGACTGATGCACAGCCTTGGAATAGATCAGCAGAAAACCATTGAGTCTTTGCAGAAC
  AAGAGCTATAACCACTTTGCTGCCATTTATTTCTTGTTGGTGGAGCGCCTGAAATCACAT
  CGGAGCAGTTTCCCAGTGGAGCAGAGACTTGATGGCCGCCAGCGTCGGCCTAGCACCATT
  GCTGAGCAAACAGTTGCCAAGGCACAGACTGTGGGGCTCCCAGTGACCATGCATTCACCG
  AACATGAGGCTGCTGCGATCTGCCCTCCTCCCCCAGGCATCCAACGTGGAGGCCTTTTCA
  TTTCCAGCATCTGGCTGTCAGGCGGAAGCTGCATTCATGGAAGAAGAGTGTGTGGACACT
  CCAAAGGTCAATGGCTGTCTGCTTGACCCTGTGCCTCCTGTCCTGGTGCGGAAGGGATGC
  CAGTCACTGCCCAGCAACATGATGGAGACCTCCATTGACGAAGGGCTGGAGACAGAAGGA
  GAGGCCGAGGAAGACCCCGCTCATGCCTTTGAGGCATTTCAGTCCACACGCAGCGGGCAG
  AGACGGCACACTCTGTCAGAAGTGACCAATCAACTGGTCGTGATGCCTGGGGCAGGGAAA
  ATTTTCTCCATGAATGACAGCCCCTCCCTTGACAGTGTGGACTCTGAGTATGATATGGGG
  TCTGTTCAGAGGGACCTGAACTTTCTGGAAGACAACCCTTCCCTTAAGGACATCATGTTA
  GCCAATCAGCCTTCACCCCGCATGACATCTCCCTTCATAAGCCTGAGACCTACCAACCCA
  GCCATGCAGGCTCTGAGCTCCCAGAAACGAGAGGTCCACAACAGGTCTCCAGTGAGCTTC
  AGAGAGGGCCGCAGAGCATCAGATACCTCCCTCACCCAGGGAATTGTAGCATTTAGACAA
  CATCTTCAGAATCTGGCTAGAACCAAAGGAATTCTAGAGTTGAACAAAGTGCAGTTGTTG
  TATGAACAAATAGGACCGGAGGCAGACCCTAACCTGGCGCCGGCGGCTCCTCAGCTCCAG
  GACCTTGCTAGCAGCTGCCCTCAGGAAGAAGTTTCTCAGCAGCAGGAAAGCGTCTCCACT
  CTCCCTGCCAGCGTGCATCCCCAGCTGTCCCCACGGCAGAGCCTGGAGACCCAGTACCTG
  CAGCACAGACTCCAGAAGCCCAGCCTTCTGTCAAAGGCCCAGAACACCTGTCAGCTTTAT
  TGCAAAGAACCACCGCGGAGCCTTGAGCAGCAGCTGCAGGAACATAGGCTCCAGCAGAAG
  CGACTCTTTCTTCAGAAGCAGTCTCAACTGCAGGCCTATTTTAATCAGATGCAGATAGCA
  GAGAGCTCCTACCCACAGCCAAGTCAGCAGCTGCCCCTTCCCCGCCAGGAGACTCCACCG
  CCTTCTCAGCAGGCCCCACCGTTCAGCCTGACCCAGCCCCTGAGCCCCGTCCTGGAGCCT
  TCCTCCGAGCAGATGCAATACAGCCCTTTCCTCAGCCAGTACCAAGAGATGCAGCTTCAG
  CCCCTGCCCTCCACTTCCGGTCCCCGGGCTGCTCCTCCTCTGCCCACGCAGCTACAGCAG
  CAGCAGCCGCCACCGCCACCACCCCCTCCACCACCACGACAGCCAGGAGCTGCCCCAGCC
  CCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCGCTGCTTCCCCTGCGCCAGAC
  TATCCCACTCCCTGTCAGTATCCTGTGGATGGAGCCCAGCAGAGCGACCTAACGGGGCCA
  GACTGTCCCAGAAGCCCAGGACTGCAAGAGGCCCCCTCCAGCTACGACCCACTAGCCCTC
  TCTGAGCTACCTGGACTCTTTGATTGTGAAATGCTAGACGCTGTGGATCCACAACACAAC
  GGGTATGTCCTGGTGAATTAG
  
  Clone variation with respect to NM_015191.1
  146 a=>t;147 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_015191 unedited
  CCCGCCGTTGTGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGCGCGCGCC
  TGGCTGGGCCCTGCGGAGCGGGAGGGAAGGAGCGAAGGAGCGAAGGAGCAAGCGGAGCGGCCGTCGCCCA
  AGCCAAGCCGCGCTGCCAACCCTCCCGCCCGCCCGCGCTCCTGTCCGCCGTGTCTAGCAGCGGGGCCCAG
  CATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGGTGGGGGTTCTACGACATCC
  GAGGGGCACGCTTGGGCCAAGGGCAACTTCGCTGTGGTGGAAGCTGGGGCCGGGCACCCGAATCACCAAG
  AACGGAAGGTGGGCAATAAGGATAATCATTAATTTCTAAGCTTGGATGCAGTGGAACCTGGGAAAAAACC
  TACCGAAAAGACACAAATAAGGAAAAGTGTAAAACCCCCCCCTCATATATCAAATTTTTTCGGGAAGGGG
  AAACCAAAGTTTTGGTTATCCCTTTGTGACGAAATTGCCCAAAAGGGGAAAATTTTTACAATACTGGGAA
  ACCGGGCCGTTAAAAAGACTCAAAACCCAGGAAAAATTTGGGAAAACCCGGTCGGGTGAAATTGGGCCGG
  GGGCAAAAAAATGGCCCCGGGACCCAAACTAAAAAATTCCGGGGGGATAACCACGGGAATATAAAAATAG
  AATTTTGTGTTTGGGAAATTTTTTTAAAGGGGGGAACGCGGGGCACAGGGGGGGGGAGCCCCCCCTATTA
  TGCCCCCAAATTTTTTTTTAGGGCGCACTTTAAAGACACACACCTGGCAGCTCGGGTGATGTGAGTGTTT
  CTTATTACTGTCGTGGAGCGTCCTTCGATGGAGCACACATTCATTATTATTGCGAAGATATGTGAGACAG
  AATGCGATGCCTGCTCATCATTGCTA
  
  Kinase domain sequence

  >SC323400 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCCTGMGCATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG
  AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGCGCGCGCCTGGCTGG
  GCCCTGCGGAGCGGGAGGGAAGGAGCGAAGGAGCGAAGGAGCAAGCGGAGCGGCCGTCGCCCAAGCCAAG
  CCGCGCTGCCAACCCTCCCGCCCGCCCGCGCTCCTGTCCGCCGTG
  
• Restriction Sites: Please inquire
• ACCN: NM_015191
• Insert Size: 4730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015191.1, NP_056006.1
• RefSeq Size: 5694 bp
• RefSeq ORF: 2781 bp
• Locus ID: 23235
• UniProt ID: Q9H0K1
• Cytogenetics: 11q23.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.[UniProtKB/Swiss-Prot Function]