Smek1 (PPP4R3A) (NM_032560) Human Tagged ORF Clone

SKU RC221040

SMEK1 (Myc-DDK-tagged)-Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Smek1
• Synonyms: FLFL1; KIAA2010; MSTP033; PP4R3A; smk-1; smk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221040 representing NM_032560; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGCA; CCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGA; CGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACAACAGGACACTCTGATT; GTGTGGTCTGAAGCAGAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTT; GGGAGAAAATATGTCAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGATGAATC; TGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAATTGCCATCTTGTGAATTAAGTCGCCTT; GAAGAAATTGCAGAACTTGTGGCATCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCAC; TAGAAAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAGATTTGGAAAATATTGA; AGGACTGCACCACTTGTATGAAATTATCAAAGGCATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTT; ATGTTCTCTGAAGAATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCACAACCAC; GAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAAGTGATTCCCATATCAGATCCTGAGCT; GAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTC; TTTGAAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTAGAGATTGTTGGCATGT; TGCAGGAAGATGAAAAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAA; AAGACAGGAATTGGTTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGA; GATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATG; ATACACAGGTGCGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACG; AGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGATATTTTGCTCATCAACCTCATTATAGAACAT; ATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTTATGGGCCTGCTTCGAACTTTAGTTG; ACCCAGAGAACATGCTAGCCACTGCCAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCA; CTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCTAGTAAAGATGATTTT; CAGACTGCCCAACTATTGGCACTTGTATTGGAATTGTTAACATTTTGTGTGGAGCACCATACCTACCACA; TAAAGAACTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCATGCTTT; CTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAAAGATGAGTTTTACAACCGCTAC; ATAATGAAAAGTTTTTTGTTTGAACCAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGA; TGAACTCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATATAAAATCATTAACTGCTCATGT; AATTGAAAATTACTGGAAAGCACTGGAAGATGTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGA; TTTGAACAACAAAGAGAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACA; GATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGTTTAACACAGATGAAGATGACAT; GGAAGATGGAGAAGCTGTAGTGTCTCCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATA; AGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTCTGAAAACAAACCTTT; CTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTGTCCAGTGGAACGAAGACTAACCTCACCAGCCAGTC; ATCTACAACAAATCTGCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCC; GTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATC; CTGATGATGATGAAGATGATGATGAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAA; ATTTGATTCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221040 representing NM_032560; Red=Cloning site Green=Tags(s); ; MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLI; VWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRL; EEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEV; MFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSV; FEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNR; DAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEH; MICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDF; QTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRY; IMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLR; FEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPI; SKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGS; VPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032560
• ORF Size: 2460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032560.4
• RefSeq Size: 4449 bp
• RefSeq ORF: 2462 bp
• Locus ID: 55671
• Cytogenetics: 14q32.12
• Domains: DUF625
• MW: 93.7 kDa
• Summary: Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function]