Smek1 (PPP4R3A) (NM_032560) Human Untagged Clone

SKU SC104547

SMEK1 (untagged)-Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Smek1
• Synonyms: FLFL1; KIAA2010; MSTP033; PP4R3A; smk-1; smk1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032560 edited; CGCCCCTCGCCTCTCCAGGGCCCCTTTCCTGGGCGTCTACTGGCGGGGCCCCCGCCCCGG; TTCCCGGGCGGCACGATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAG; GACCGGCAGTGGGACGACCGGGGCACCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTG; AAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGACGGTTCTCTACTTTTAGAGTCGAAA; ATAAATCCTAACACTGCATACCAGAAACAACAGGACACTCTGATTGTGTGGTCTGAAGCA; GAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTTGGGAG; AAAATATGTCAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGAT; GAATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAATTGCCATCTTGT; GAATTAAGTCGCCTTGAAGAAATTGCAGAACTTGTGGCATCATCTTTACCTTCACCTCTT; CGTCGTGAAAAACTTGCACTGGCACTAGAAAATGAGGGTTATATTAAAAAGCTCCTGGAG; CTTTTTCATGTGTGTGAAGATTTGGAAAATATTGAAGGACTGCACCACTTGTATGAAATT; ATCAAAGGCATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTCTCTGAAGAA; TGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCACAACCACGAAAA; CACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAAGTGATTCCCATATCAGATCCT; GAGCTGAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTA; CCAACTCCTTCGGTCTTTGAAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTC; AATAAGGTAGAGATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACAGATTTGTTT; GCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGGTTAACTTTTTA; AAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGAGATGCTTTTTTCAAG; ACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATGATACA; CAGGTGCGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATG; GTACGAGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGATATTTTGCTCATCAAC; CTCATTATAGAACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTT; ATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAAAACA; GAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCT; TTACTAGCAAATACAACAGAAGACAAACCTAGTAAAGATGATTTTCAGACTGCCCAACTA; TTGGCACTTGTATTGGAATTGTTAACATTTTGTGTGGAGCACCATACCTACCACATAAAG; AACTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCAT; GCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAAAGATGAG; TTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAACCAGTAGTGAAAGCATTTCTC; AACAATGGATCCCGCTACAATCTGATGAACTCTGCCATAATAGAGATGTTTGAATTTATT; AGAGTGGAAGATATAAAATCATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTG; GAAGATGTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGA; GAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACAGATAT; CGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGTTTAACACAGATGAAGAT; GACATGGAAGATGGAGAAGCTGTAGTGTCTCCATCTGACAAAACTAAAAATGATGATGAT; ATTATGGATCCAATAAGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAG; GAAGTGCTTCTGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTG; TCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCTGCCTGGTTCT; CCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAAATACA; TCTCAGACGGCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATCCTGAT; GATGATGAAGATGATGATGAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAA; GCAAAATTTGATTCATAATAATGGCAACGGCCTAGGATCAGTACCTGTTGAAAAAAACTG; GTTCTCCACCCCTCCCCCATACAAAATCCACAAAAAAGCGCAGTGGTCTCTTGTGAATGA; CTGACACAGATCAGCCTCTTACACTTGACTTCTGCTCATCAAGTGCCAATTCAATGGAGC; AGGAGGAGGGGATATCATATATTTAGGGGAAAGACTTAAGCCTTTGAGCTCTCCAGCTTG; GACCACACATTGCCCTTTTCTCAGGGAAGGAAATGGAAACAAAAAGCCAACAGGGCAGGG; GTTTTGTAAGTGGAACTCTGGATTGACTGGTCAGTTGCTACAATCAGAATATGCTTTCTT; GGACCATGTTTGAGACTCAGAAGAATGGGCCTTTCTGCCATAATTCTTCACTAGTCAAGA; ATGCCAGCAGTTTCTTTGTATAAAGAGACCTGCCTTTAAAATCATACATTCTGAACATTT; TAGTCAAGCTACAACAGGTTTGGAAAACCTCTGTGGGGGAGGGGCGAGTATAAAGTTTTC; CTCTTTTTTAACTGTTCCCTTTGCCCTTCAAACTGCAGATATTTTTTTTTTTAAGTGGGG; ACTTCTCCCTACTTGATTAAAGATTGAGTGGAATTCTAGATGTGGTCATTTGTGTCATAA; TTTTTTTGTTTTATTTTGTTTTTGATTTTTTTTTTCCTCCCCTGAGTGTATGCTTAGTTG; TTGAGTATATATATTTGGGACCATTAAAACTTTTTTTGATGTAATATAACCTAACGTTGT; GCTGGTACCTGTTTTACCATGTGTAATTTTTGTTCTACATCACAGTTCTTAATTTGTTTA; GAGTTTTATGAAAGATGGTATAGTTTTTATTGACAAAAGCAAAGTAATCTTACAACTATG; TGCATACAAAAGCAATACTATTTTGTGACTAAATATTTTATATTAAAATTTACATCAGCA; ACTGTCTTGAGAATTCAGGGAAATAGAATGGAATTTAAAACTTCAACAGTTTTGTTAAAT; CTAGAAACATGAAATTAGTATTCCAAAGAGATTCTGAAATTTCTTTTCTTGGGGAAATGA; CGGTACATTAAATCAAAATTGAGGATGGATGATTTAAAAACATTTGACTTTTTAATAATA; AAAAGAAAAGTGAAGAGTAAGAGAAATTGTATTAGTTGTATGTTTCTTGTTTTTGTTTTT; AACTACTCCCTTTGGAATACCAGTTTGACTTGTAAGTGGTGGTTTCTAAATAAATACCTG; GCCAGAGGACCATCACCTGTCACCTTATTAAAACAGTATTTTTAAATAGGTTCTTTTTGT; TGTCTTAGATGTGATCAAATTATTTCAAGGAAAATGAAAATAATTCTACTTACCCTCACT; TTTTAAAGTGAAGAGTTTAGAACTTAAGAATATATTTTCAAATGTGAATAAAACTTCATA; TCCAGGAAAGGATGCTATTTTAAGTATCATATACTTTAAGAGTATCTAGCAAGCGCTGTG; TGCTTCTCTGTCTTGTCTTGTTTATTCCTCAGTAAATTACAATGGTGGTACCAAAAACAA; GACTATACTGCATAGTTGGATAGAAAACCAGAAAGTGTCAACTTGCTACAGTCATTAAAA; GTTTTATTATTGGCTGAAAAAAAAAAAAAAAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_032560
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_032560.3.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032560.3, NP_115949.3
• RefSeq Size: 4449 bp
• RefSeq ORF: 2463 bp
• Locus ID: 55671
• Cytogenetics: 14q32.12
• Domains: DUF625
• Summary: Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longer protein (isoform 1).