Human Smek1 (PPP4R3A) (NM_032560) AAV Particle

SKU RC221040A1V

AAV ORF Particle, Serotype AAV-2, SMEK1 (Myc-DDK-tagged)-Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: SMEK1
• Synonyms: FLFL1; KIAA2010; MSTP033; PP4R3A; smk-1; smk1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221040 representing NM_032560; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGCA; CCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGA; CGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACAACAGGACACTCTGATT; GTGTGGTCTGAAGCAGAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTT; GGGAGAAAATATGTCAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGATGAATC; TGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAATTGCCATCTTGTGAATTAAGTCGCCTT; GAAGAAATTGCAGAACTTGTGGCATCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCAC; TAGAAAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAGATTTGGAAAATATTGA; AGGACTGCACCACTTGTATGAAATTATCAAAGGCATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTT; ATGTTCTCTGAAGAATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCACAACCAC; GAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAAGTGATTCCCATATCAGATCCTGAGCT; GAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTC; TTTGAAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTAGAGATTGTTGGCATGT; TGCAGGAAGATGAAAAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAA; AAGACAGGAATTGGTTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGA; GATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATG; ATACACAGGTGCGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACG; AGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGATATTTTGCTCATCAACCTCATTATAGAACAT; ATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTTATGGGCCTGCTTCGAACTTTAGTTG; ACCCAGAGAACATGCTAGCCACTGCCAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCA; CTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCTAGTAAAGATGATTTT; CAGACTGCCCAACTATTGGCACTTGTATTGGAATTGTTAACATTTTGTGTGGAGCACCATACCTACCACA; TAAAGAACTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCATGCTTT; CTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAAAGATGAGTTTTACAACCGCTAC; ATAATGAAAAGTTTTTTGTTTGAACCAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGA; TGAACTCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATATAAAATCATTAACTGCTCATGT; AATTGAAAATTACTGGAAAGCACTGGAAGATGTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGA; TTTGAACAACAAAGAGAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACA; GATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGTTTAACACAGATGAAGATGACAT; GGAAGATGGAGAAGCTGTAGTGTCTCCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATA; AGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTCTGAAAACAAACCTTT; CTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTGTCCAGTGGAACGAAGACTAACCTCACCAGCCAGTC; ATCTACAACAAATCTGCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCC; GTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATC; CTGATGATGATGAAGATGATGATGAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAA; ATTTGATTCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221040 representing NM_032560; Red=Cloning site Green=Tags(s); ; MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLI; VWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRL; EEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEV; MFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSV; FEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNR; DAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEH; MICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDF; QTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRY; IMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLR; FEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPI; SKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGS; VPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_032560
• ORF Size: 2460 bp
• Buffer: PBS with 0.001% Pluronic F8277
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_032560.3
• RefSeq Size: 4449 bp
• RefSeq ORF: 2462 bp
• Locus ID: 55671
• Cytogenetics: 14q32.12
• MW: 93.7 kDa