NRAMP1 (SLC11A1) (NM_000578) Human Tagged ORF Clone
SKU
RC219733
SLC11A1 (Myc-DDK-tagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1)
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | NRAMP1 |
Synonyms | LSH; NRAMP; NRAMP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC219733 representing NM_000578
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGTGACAAGGGTCCCCAAAGGCTAAGCGGGTCCAGCTATGGTTCCATCTCCAGCCCGACCAGCC CGACCAGCCCAGGGCCACGGCAAGCACCTCCCAGAGAGACCTACCTGAGTGAGAAGATCCCCATCCCAGA CACAAAACCGGGCACCTTCAGCCTGCGGAAGCTATGGGCCTTCACGGGGCCTGGCTTCCTCATGAGCATT GCTTTCCTGGACCCAGGAAACATCGAGTCAGATCTTCAGGCTGGCGCCGTGGCGGGATTCAAACTTCTCT GGGTGCTGCTCTGGGCCACCGTGTTGGGCTTGCTCTGCCAGCGACTGGCTGCACGTCTGGGCGTGGTGAC AGGCAAGGACTTGGGCGAGGTCTGCCATCTCTACTACCCTAAGGTGCCCCGCACCGTCCTCTGGCTGACC ATCGAGCTAGCCATTGTGGGCTCCGACATGCAGGAAGTCATCGGCACGGCCATTGCATTCAATCTGCTCT CAGCTGGACGAATCCCACTCTGGGGTGGCGTCCTCATCACCATCGTGGACACCTTCTTCTTCCTCTTCCT CGATAACTACGGGCTGCGGAAGCTGGAAGCTTTTTTTGGACTCCTTATAACCATTATGGCCTTGACCTTT GGCTATGAGTATGTGGTGGCGCGTCCTGAGCAGGGAGCGCTTCTTCGGGGCCTGTTCCTGCCCTCGTGCC CGGGCTGCGGCCACCCCGAGCTGCTGCAGGCGGTGGGCATTGTTGGCGCCATCATCATGCCCCACAACAT CTACCTGCACTCGGCCCTGGTCAAGTCTCGAGAGATAGACCGGGCCCGCCGAGCGGACATCAGAGAAGCC AACATGTACTTCCTGATTGAGGCCACCATCGCCCTGTCCGTCTCCTTTATCATCAACCTCTTTGTCATGG CTGTCTTTGGGCAGGCCTTCTACCAGAAAACCAACCAGGCTGCGTTCAACATCTGTGCCAACAGCAGCCT CCACGACTACGCCAAGATCTTCCCCATGAACAACGCCACCGTGGCCGTGGACATTTACCAGGGGGGCGTG ATCCTGGGCTGCCTGTTCGGCCCCGCGGCCCTCTACATCTGGGCCATAGGTCTCCTGGCGGCTGGGCAGA GCTCCACCATGACGGGCACCTACGCGGGACAGTTCGTGATGGAGGGCTTCCTGAGGCTGCGGTGGTCACG CTTCGCCCGTGTCCTCCTCACCCGCTCCTGCGCCATCCTGCCCACCGTGCTCGTGGCTGTCTTCCGGGAC CTGAGGGACTTGTCGGGCCTCAATGATCTGCTCAACGTGCTGCAGAGCCTGCTGCTCCCGTTCGCCGTGC TGCCCATCCTCACGTTCACCAGCATGCCCACCCTCATGCAGGAGTTTGCCAATGGCCTGCTGAACAAGGT CGTCACCTCTTCCATCATGGTGCTAGTCTGCGCCATCAACCTCTACTTCGTGGTCAGCTATCTGCCCAGC CTGCCCCACCCTGCCTACTTCGGCCTTGCAGCCTTGCTGGCCGCAGCCTACCTGGGCCTCAGCACCTACC TGGTCTGGACCTGTTGCCTTGCCCACGGAGCCACCTTTCTGGCCCACAGCTCCCACCACCACTTCCTGTA TGGGCTCCTTGAAGAGGACCAGAAAGGGGAGACCTCTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219733 representing NM_000578
Red=Cloning site Green=Tags(s) MTGDKGPQRLSGSSYGSISSPTSPTSPGPRQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSI AFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLT IELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTF GYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREA NMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGV ILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRD LRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPS LPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000578 |
ORF Size | 1650 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_000578.2, NP_000569.2 |
RefSeq Size | 2573 bp |
RefSeq ORF | 1653 bp |
Locus ID | 6556 |
UniProt ID | P49279 |
Cytogenetics | 2q35 |
Domains | Nramp |
Protein Families | Transmembrane |
Protein Pathways | Lysosome |
MW | 59.7 kDa |
Summary | This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC219733L1 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1), Myc-DDK-tagged | 10 ug |
$1,068.00
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RC219733L2 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1), mGFP tagged | 10 ug |
$1,068.00
|
|
RC219733L3 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1), Myc-DDK-tagged | 10 ug |
$1,068.00
|
|
RC219733L4 | Lenti ORF clone of Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1), mGFP tagged | 10 ug |
$1,068.00
|
|
RG219733 | SLC11A1 (tGFP-tagged) - Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1) | 10 ug |
$968.00
|
|
SC310275 | SLC11A1 (untagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1) | 10 ug |
$769.00
|
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.