GIRK1 (KCNJ3) (NM_002239) Human Tagged Lenti ORF Clone

SKU
RC205322L1
Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$766.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GIRK1
Synonyms GIRK1; KGA; KIR3.1
Vector pLenti-C-Myc-DDK
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205322).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_002239
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002239.2
RefSeq Size 4740 bp
RefSeq ORF 1506 bp
Locus ID 3760
UniProt ID P48549
Cytogenetics 2q24.1
Domains IRK
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 56.7 kDa
Summary Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012]
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SKU Description Size Price
RC205322 KCNJ3 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) 10 ug
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RC205322L3 Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged 10 ug
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RC205322L4 Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged 10 ug
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$489.00 MSRP $665.00 MSRP $665.00
SC118769 KCNJ3 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.