GIRK1 (KCNJ3) (NM_002239) Human Tagged Lenti ORF Clone
SKU
RC205322L1
Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged
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LentiORF®
LentiORF®
Expression-ready ORF plasmid in lenti backbone
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | GIRK1 |
Synonyms | GIRK1; KGA; KIR3.1 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chl (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205322).
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Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002239 |
ORF Size | 1503 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_002239.2 |
RefSeq Size | 4740 bp |
RefSeq ORF | 1506 bp |
Locus ID | 3760 |
UniProt ID | P48549 |
Cytogenetics | 2q24.1 |
Domains | IRK |
Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
MW | 56.7 kDa |
Summary | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC205322 | KCNJ3 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) | 10 ug |
$466.00
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RC205322L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged | 10 ug |
$766.00
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RC205322L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged | 10 ug |
$766.00
|
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RC205322L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged | 10 ug |
$766.00
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RG205322 | KCNJ3 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) | 10 ug |
$489.00
MSRP
$665.00
MSRP
$665.00
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SC118769 | KCNJ3 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) | 10 ug |
$701.00
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.