BMI1 (NM_005180) Human Tagged ORF Clone
BMI1 (Myc-DDK-tagged)-Human BMI1 polycomb ring finger oncogene (BMI1)
|Product Name||BMI1 (NM_005180) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC202871 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202871 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||978 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_005180.1, NM_005180.2, NM_005180.3, NM_005180.4, NM_005180.5, NM_005180.6, NM_005180.7, NM_005180.8, NP_005171.4|
|RefSeq Size||3435 bp|
|RefSeq ORF||981 bp|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors|
|Gene Summary||This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
Regulation of the Potential Marker for Intestinal Cells, Bmi1, by ß-Catenin and the Zinc Finger Protein KLF4: IMPLICATIONS FOR COLON CANCER
,Tianxin Yu, Xi Chen, Wen Zhang, Deannon Colon, Jiandang Shi, Dana Napier, Piotr Rychahou, Wange Lu, Eun Y. Lee, Heidi L. Weiss, B. Mark Evers, and Chunming Liu,
J. Biol. Chem., Feb 2012; 287: 3760 - 3768.
|cDNA Clone Resources|
|SC116894||BMI1 (untagged)-Human BMI1 polycomb ring finger oncogene (BMI1)||
|RG202871||BMI1 (GFP-tagged) - Human BMI1 polycomb ring finger oncogene (BMI1)||
|RC202871L1||Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), Myc-DDK-tagged||
|RC202871L2||Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), mGFP tagged||
|RC202871L3||Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), Myc-DDK-tagged||
|RC202871L4||Lenti ORF clone of Human BMI1 polycomb ring finger oncogene (BMI1), mGFP tagged||