Sp100 (NM_013673) Mouse Tagged ORF Clone

SKU MR220765

Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (Sp100)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Sp100
• Synonyms: A430075G10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220765 representing NM_013673; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGGGAGCGACGGCAGCCCCAGGATGTCCACTGAACAGGAGAACACAGAGATGCATCTCATTGAAT; GTATGTTAAAGCACTTCAAGACACAGAAAGTGGCCATTTCTAATGCGATAAGAAGTACATTTCCTTTTCT; TGAGAGCCTCCGAGACCATGAATTCATCACTGGTAAAATGTATGAAGATTTGCTAGATTCCTGTAGATCG; CTGGTCCCTGTGGACAAAGTGATCTACAGAGCCCTGGAGGAACTGGAGAAGAAATTTGACATGACGGTAC; TGTGCGAATTGTTCAATGAGGTCAACATGGAGAAGTACCCCGATTTGAATCTCATTCGTAGAAGCTTTGG; ATGTGTGTTTCCCAATGAATTGTGTTTCCAAGGAATTGATGGAGGGAACCCAAACTCCCAGCTAAGTCTT; GAACAAGGACCTGGTGCCAGCTATTCTCAAGGAAGCCCGAATGGGTCATCCTTAGATCTCTCCGCATCTG; AAGGCTGGAGAAGTAATGATAGAAGAAATTCCAACCTCATGCAGGCAAACCAAACTGAGAATCATCAGCT; TGCCGAATCACCAGGTCACCTTGATTCCTGTGAACTCCAAGTCCAACTGAATAACAGAGATGCTACCCCA; GAGTCTTGCAGCCTATTGCCTCAGAATGAAGAAAGAGCTGTGCAACTCAACTATGAACTCCAGATTAATC; CCTGTTTTGTGCAACTGGTAGATGTAAAGAAGGAAAATTCATCATTTTCCTTGGCTGGTAACCAACAAAC; CCGAGCAAGGACCAACCAAAATGAGGACTCTGAGATAATAGAACTCAGCAGTGGGGATTCTGATAATGGA; GAGAACTTCTCAGAAGCTACAACCACAGTCCCCTCCCAGCCAGCACCTGCATATTCCAGAAAACCTCCAA; CACTGAGAAGAGACAGAGGAGGAGATACTTCTGACACTGAGAGCTCTATCATCATTAGAAGACGGAAAAG; GACAGGACGGAAAAAGAGAGAGAGACTTGGAAGCTACTTAATACGGAACATCAAAATCCCAATGAAACCA; AGCTGGAAAACAGCTTTCCTAGCAAGAAGTGCCAATCCTTCTTCACAACGACGCAGAAAAAGAGGTCCGA; GAATCCCCAGAGAAGAAAATGCGGATTTTGGAGGAGCTGAGCTTCCTGTGGTATGTGGCAACGCTCAGGG; CTTCCTGGATAAGGAGAAATTTAAGCAAGGCATCTATGTGAGGAGTATACGGGGCAAGACAGGGCGGTTA; TTCACTCCCATGGACTTTGAAATCGAAGGAAACTGTGAAAAAGCCAAGAACTGGCGGCAAAGCATCCGCT; GCAAGGGATGGACCCTGAGAGAACTGATACAGAAAGGAGTACTACAAGACCCACCAAGGAAAAAGAAGGA; AACTCCACGAAACCCAAGGCAAACAAGGAGACAGAAATGTGAACTGCTTCTTTTGACTATCTATTGCTAT; CCAAAAAGTGGCTTTTTTATCCCCAAACCAAAGCAAAGGAAAGAGAAGTTTCCAGACCTCCGGGAGCACA; TGTGGTTAAACAAAATCAAGAATAGGCTGAATAAGAAGGCATACCATTCGGTACGACGCTTTGTGGAGGA; CATGCGCCTCATCTTTCACAACCACAGCATATTTTATAAAAAAAGGTTCAAAAATCTTGGAATCATAGTA; GGAAAGAAATTTGAGAAGAATTTCAAAAGGATTTTTTCAATCGAGGATACAAGCGAGCAACATGAACCGT; GCAACCACACCGTTTTGTTAACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220765 representing NM_013673; Red=Cloning site Green=Tags(s); ; MEGSDGSPRMSTEQENTEMHLIECMLKHFKTQKVAISNAIRSTFPFLESLRDHEFITGKMYEDLLDSCRS; LVPVDKVIYRALEELEKKFDMTVLCELFNEVNMEKYPDLNLIRRSFGCVFPNELCFQGIDGGNPNSQLSL; EQGPGASYSQGSPNGSSLDLSASEGWRSNDRRNSNLMQANQTENHQLAESPGHLDSCELQVQLNNRDATP; ESCSLLPQNEERAVQLNYELQINPCFVQLVDVKKENSSFSLAGNQQTRARTNQNEDSEIIELSSGDSDNG; ENFSEATTTVPSQPAPAYSRKPPTLRRDRGGDTSDTESSIIIRRRKRTGRKKRERLGSYLIRNIKIPMKP; SWKTAFLARSANPSSQRRRKRGPRIPREENADFGGAELPVVCGNAQGFLDKEKFKQGIYVRSIRGKTGRL; FTPMDFEIEGNCEKAKNWRQSIRCKGWTLRELIQKGVLQDPPRKKKETPRNPRQTRRQKCELLLLTIYCY; PKSGFFIPKPKQRKEKFPDLREHMWLNKIKNRLNKKAYHSVRRFVEDMRLIFHNHSIFYKKRFKNLGIIV; GKKFEKNFKRIFSIEDTSEQHEPCNHTVLLT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013673
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013673.4, NP_038701.2
• RefSeq Size: 2158 bp
• RefSeq ORF: 1776 bp
• Locus ID: 20684
• Cytogenetics: 1 43.6 cM
• MW: 68.6 kDa
• Summary: Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function]