Sp100 (NM_013673) Mouse Tagged Lenti ORF Clone

SKU
MR220765L4
Lenti ORF clone of Sp100 (mGFP-tagged) - Mouse nuclear antigen Sp100 (Sp100)
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

    Click here to learn more.

$850.00
3 Weeks*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sp100
Synonyms A430075G10Rik
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR220765).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_013673
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013673.3, NP_038701.2
RefSeq Size 2158 bp
RefSeq ORF 1776 bp
Locus ID 20684
Cytogenetics 1 43.6 cM
Summary Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function]
Write Your Own Review
You're reviewing:Sp100 (NM_013673) Mouse Tagged Lenti ORF Clone
Your Rating
SKU Description Size Price
MC219399 Sp100 (untagged) - Mouse nuclear antigen Sp100 (Sp100), (10ug) 10 ug
$606.00
MG220765 Sp100 (tGFP-tagged) - Mouse nuclear antigen Sp100 (Sp100), (10ug) 10 ug
$750.00
MR220765 Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (Sp100) 10 ug
$289.00 MSRP $550.00 MSRP $550.00
MR220765L3 Lenti ORF clone of Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (Sp100) 10 ug
$850.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.