Sp100 (NM_013673) Mouse Untagged Clone

SKU MC219399

Sp100 (untagged) - Mouse nuclear antigen Sp100 (Sp100), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Sp100
• Synonyms: A430075G10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219399 representing NM_013673; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGGGAGCGACGGCAGCCCCAGGATGTCCACTGAACAGGAGAACACAGAGATGCATCTCATTGAAT; GTATGTTAAAGCACTTCAAGACACAGAAAGTGGCCATTTCTAATGCGATAAGAAGTACATTTCCTTTTCT; TGAGAGCCTCCGAGACCATGAATTCATCACTGGTAAAATGTATGAAGATTTGCTAGATTCCTGTAGATCG; CTGGTCCCTGTGGACAAAGTGATCTACAGAGCCCTGGAGGAACTGGAGAAGAAATTTGACATGACGGTAC; TGTGCGAATTGTTCAATGAGGTCAACATGGAGAAGTACCCCGATTTGAATCTCATTCGTAGAAGCTTTGG; ATGTGTGTTTCCCAATGAATTGTGTTTCCAAGGAATTGATGGAGGGAACCCAAACTCCCAGCTAAGTCTT; GAACAAGGACCTGGTGCCAGCTATTCTCAAGGAAGCCCGAATGGGTCATCCTTAGATCTCTCCGCATCTG; AAGGCTGGAGAAGTAATGATAGAAGAAATTCCAACCTCATGCAGGCAAACCAAACTGAGAATCATCAGCT; TGCCGAATCACCAGGTCACCTTGATTCCTGTGAACTCCAAGTCCAACTGAATAACAGAGATGCTACCCCA; GAGTCTTGCAGCCTATTGCCTCAGAATGAAGAAAGAGCTGTGCAACTCAACTATGAACTCCAGATTAATC; CCTGTTTTGTGCAACTGGTAGATGTAAAGAAGGAAAATTCATCATTTTCCTTGGCTGGTAACCAACAAAC; CCGAGCAAGGACCAACCAAAATGAGGACTCTGAGATAATAGAACTCAGCAGTGGGGATTCTGATAATGGA; GAGAACTTCTCAGAAGCTACAACCACAGTCCCCTCCCAGCCAGCACCTGCATATTCCAGAAAACCTCCAA; CACTGAGAAGAGACAGAGGAGGAGATACTTCTGACACTGAGAGCTCTATCATCATTAGAAGACGGAAAAG; GACAGGACGGAAAAAGAGAGAGAGACTTGGAAGCTACTTAATACGGAACATCAAAATCCCAATGAAACCA; AGCTGGAAAACAGCTTTCCTAGCAAGAAGTGCCAATCCTTCTTCACAACGACGCAGAAAAAGAGGTCCGA; GAATCCCCAGAGAAGAAAATGCGGATTTTGGAGGAGCTGAGCTTCCTGTGGTATGTGGCAACGCTCAGGG; CTTCCTGGATAAGGAGAAATTTAAGCAAGGCATCTATGTGAGGAGTATACGGGGCAAGACAGGGCGGTTA; TTCACTCCCATGGACTTTGAAATCGAAGGAAACTGTGAAAAAGCCAAGAACTGGCGGCAAAGCATCCGCT; GCAAGGGATGGACCCTGAGAGAACTGATACAGAAAGGAGTACTACAAGACCCACCAAGGAAAAAGAAGGA; AACTCCACGAAACCCAAGGCAAACAAGGAGACAGAAATGTGAACTGCTTCTTTTGACTATCTATTGCTAT; CCAAAAAGTGGCTTTTTTATCCCCAAACCAAAGCAAAGGAAAGAGAAGTTTCCAGACCTCCGGGAGCACA; TGTGGTTAAACAAAATCAAGAATAGGCTGAATAAGAAGGCATACCATTCGGTACGACGCTTTGTGGAGGA; CATGCGCCTCATCTTTCACAACCACAGCATATTTTATAAAAAAAGGTTCAAAAATCTTGGAATCATAGTA; GGAAAGAAATTTGAGAAGAATTTCAAAAGGATTTTTTCAATCGAGGATACAAGCGAGCAACATGAACCGT; GCAACCACACCGTTTTGTTAACCTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013673
• Insert Size: 1776 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013673.4, NP_038701.2
• RefSeq Size: 2788 bp
• RefSeq ORF: 1776 bp
• Locus ID: 20684
• Cytogenetics: 1 43.6 cM
• Summary: Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).