Htra1 (NM_019564) Mouse Tagged ORF Clone

SKU MR207695

Htra1 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 1 (Htra1)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Htra1
• Synonyms: AI429470; HTRA; L56; Prss11; RSPP11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207695 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCAGTCCCTGCGTACCACGCTCCTGTCTTTGCTACTGCTGCTGCTAGCGGCTCCTTCCTTGGCGTTGC; CGTCGGGGACCGGCCGCTCGGCCCCAGCTGCCACCGTCTGTCCCGAGCACTGCGATCCCACCCGCTGCGC; CCCGCCGCCCACGGACTGCGAGGGTGGCCGCGTCCGCGACGCGTGCGGCTGCTGCGAGGTGTGCGGCGCG; CTCGAGGGTGCAGCGTGCGGCCTGCAGGAGGGTCCCTGCGGCGAGGGGCTGCAATGCGTAGTGCCCTTCG; GGGTGCCGGCCTCGGCCACAGTACGACGGCGCGCACAGGCCGGCCTGTGCGTGTGTGCCAGCAGCGAGCC; GGTGTGTGGTAGCGACGCCAAGACCTACACCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCTCCGAG; AAGCTTCGCCAGCCGCCGGTCATCGTCCTGCAGCGCGGCGCCTGCGGCCAAGGGCAGGAAGATCCCAACA; GTTTGCGTCATAAGTACAACTTTATTGCTGATGTGGTGGAGAAGATCGCCCCGGCTGTGGTTCACATTGA; ACTATATCGCAAGCTTCCTTTCTCGAAGAGGGAGGTGCCAGTGGCCAGTGGGTCAGGATTCATCGTATCG; GAGGATGGACTGATTGTGACAAATGCTCACGTGGTCACCAACAAAAACCGGGTCAAGGTTGAGCTGAAGA; ATGGAGCTACCTATGAAGCCAAAATCAAGGATGTGGATGAAAAGGCGGACATTGCGCTTATCAAGATTGA; CCACCAGGGAAAGCTGCCAGTCCTGCTGCTCGGCCGCTCCTCAGAGCTGAGACCTGGAGAATTTGTAGTT; GCCATTGGAAGCCCCTTTTCTCTTCAAAACACAGTCACCACTGGGATCGTCAGCACCACCCAGCGAGGCG; GCAAAGAGCTGGGACTTCGGAACTCCGATATGGACTACATTCAGACAGACGCTATCATCAATTATGGAAA; TTCCGGAGGCCCGTTAGTAAACCTGGATGGCGAGGTGATTGGGATTAACACCTTGAAGGTGACGGCGGGC; ATCTCCTTCGCAATTCCATCCGATAAGATAAAAAAGTTCTTGACACAGTCCCACGATCGACAGGCCAAAG; GGAAAGCTGTCACCAAGAAGAAGTATATTGGGATCCGAATGATGTCGCTCACATCTAGCAAAGCCAAAGA; GCTGAAGGACCGTCACCGAGACTTCCCGGATGTGCTCTCTGGGGCATATATCATTGAAGTCATTCCTGAC; ACCCCGGCAGAAGCCGGAGGGCTCAAGGAAAATGACGTCATCATCAGCATCAACGGACAGTCTGTGGTCA; CTGCCAATGACGTCAGCGATGTCATCAAAAAGGAGAACACCCTGAACATGGTTGTCCGCAGGGGCAATGA; AGACATTGTGATTACCGTGATTCCTGAAGAAATCGACCCC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207695 protein sequence; Red=Cloning site Green=Tags(s); ; MQSLRTTLLSLLLLLLAAPSLALPSGTGRSAPAATVCPEHCDPTRCAPPPTDCEGGRVRDACGCCEVCGA; LEGAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSDAKTYTNLCQLRAASRRSE; KLRQPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELYRKLPFSKREVPVASGSGFIVS; EDGLIVTNAHVVTNKNRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVV; AIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAG; ISFAIPSDKIKKFLTQSHDRQAKGKAVTKKKYIGIRMMSLTSSKAKELKDRHRDFPDVLSGAYIIEVIPD; TPAEAGGLKENDVIISINGQSVVTANDVSDVIKKENTLNMVVRRGNEDIVITVIPEEIDP; ; SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019564
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019564.3
• RefSeq Size: 2051 bp
• RefSeq ORF: 1443 bp
• Locus ID: 56213
• UniProt ID: Q9R118
• Cytogenetics: 7 F3
• MW: 51.2 kDa
• Summary: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves (PubMed:18551132) or not (PubMed:14973287). By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.[UniProtKB/Swiss-Prot Function]