Htra1 (NM_019564) Mouse Untagged Clone

SKU MC201064

Htra1 (untagged) - Mouse HtrA serine peptidase 1 (Htra1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Htra1
• Synonyms: AI429470; HTRA; L56; Prss11; RSPP11
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013516 sequence for NM_019564; CGGACGCGTGGGGTCCAGCCCGTGGCCGTCGGAGTCGTCATGCAGTCCCTGCGTACCACGCTCCTGTCTT TGCTACTGCTGCTGCTAGCGGCTCCTTCCTTGGCGTTGCCGTCGGGGACCGGCCGCTCGGCCCCAGCTGC CACCGTCTGTCCCGAGCACTGCGATCCCACCCGCTGCGCCCCGCCGCCCACGGACTGCGAGGGTGGCCGC GTCCGCGACGCGTGCGGCTGCTGCGAGGTGTGCGGCGCGCTCGAGGGTGCAGCGTGCGGCCTGCAGGAGG GTCCCTGCGGCGAGGGGCTGCAATGCGTAGTGCCCTTCGGGGTGCCGGCCTCGGCCACAGTACGACGGCG CGCACAGGCCGGCCTGTGCGTGTGTGCCAGCAGCGAGCCGGTGTGTGGTAGCGACGCCAAGACCTACACC AACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCTCCGAGAAGCTTCGCCAGCCGCCGGTCATCGTCCTGC AGCGCGGCGCCTGCGGCCAAGGGCAGGAAGATCCCAACAGTTTGCGTCATAAGTACAACTTTATTGCTGA TGTGGTGGAGAAGATCGCCCCGGCTGTGGTTCACATTGAACTATATCGCAAGCTTCCTTTCTCGAAGAGG GAGGTGCCAGTGGCCAGTGGGTCAGGATTCATCGTATCGGAGGATGGACTGATTGTGACAAATGCTCACG TGGTCACCAACAAAAACCGGGTCAAGGTTGAGCTGAAGAATGGAGCTACCTATGAAGCCAAAATCAAGGA TGTGGATGAAAAGGCGGACATTGCGCTTATCAAGATTGACCACCAGGGAAAGCTGCCAGTCCTGCTGCTC GGCCGCTCCTCAGAGCTGAGACCTGGAGAATTTGTAGTTGCCATTGGAAGCCCCTTTTCTCTTCAAAACA CAGTCACCACTGGGATCGTCAGCACCACCCAGCGAGGCGGCAAAGAGCTGGGACTTCGGAACTCCGATAT GGACTACATTCAGACAGACGCTATCATCAATTATGGAAATTCCGGAGGCCCGTTAGTAAACCTGGATGGC GAGGTGATTGGGATTAACACCTTGAAGGTGACGGCGGGCATCTCCTTCGCAATTCCATCCGATAAGATAA AAAAGTTCTTGACACAGTCCCACGATCGACAGGCCAAAGGGAAAGCTGTCACCAAGAAGAAGTATATTGG GATCCGAATGATGTCGCTCACATCTAGCAAAGCCAAAGAGCTGAAGGACCGTCACCGAGACTTCCCGGAT GTGCTCTCTGGGGCATATATCATTGAAGTCATTCCTGACACCCCGGCAGAAGCCGGAGGGCTCAAGGAAA ATGACGTCATCATCAGCATCAACGGACAGTCTGTGGTCACTGCCAATGACGTCAGCGATGTCATCAAAAA GGAGAACACCCTGAACATGGTTGTCCGCAGGGGCAATGAAGACATTGTGATTACCGTGATTCCTGAAGAA ATCGACCCCTAGGCAGAGGCAGGAGCCAGACTTCATGTTTCCCTCAAAGACTCCAGGGGATGGCGCATGA GAACTGAGCTGGTGACGCAGGACACCCGGGACCTTTGTCCAACATTTTGCTTGTTCAGGGAACACCCCTG CCGACAGAATCCTTCTTGATAGTTTGCGGGCAAAACAAATGTAATGTTGCAGGTGCACAGGCAGAAGTCC TGCCTTCTGTATGCTATGTATGCAGCGTGCTTTTCCTTACAAGCTTGGGCTGTTCCTGCTTACACAGTCA ACATTTGTCCCTTCCCTTAGCCTAAGTTGTCTGACTAATGCAGTTGACGGATGCGTAGGCAAGAGGAAGG TCCTCGCAGCCATGGTCTTGGGCGTGTTTACACTTTTCCTCCGAGTCGGCACTCAGAGGAATTTGATGCC CCGAGACCACAGGTGGGTGACCCTGGCTTCTGAGATGGCCAAAAATGCCTCTTAGGAATCCTGGAACCCG GAGTACAGTGACTTTGAGTTTCAGCTATTAAAATACTTCTTCATACGTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019564
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC013516, AAH13516
• RefSeq Size: 2023 bp
• RefSeq ORF: 1443 bp
• Locus ID: 56213
• UniProt ID: Q9R118
• Cytogenetics: 7 F3
• Summary: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves (PubMed:18551132) or not (PubMed:14973287). By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.[UniProtKB/Swiss-Prot Function]