Tool for PD-1 research in immunotherapy and apoptosis

PD-1 inhibits T-cell function upon binding to its ligands, PD-L1 and PD-L2, serving as a key checkpoint of the immune system. PD-1 and PD-L1 blocking mAbs have been demonstrated to improve the anti-tumor activities of T-cells in immunotherapy.

PD-1 Antibody

PD-1 UltraMab™ Antibody

SKU# UM800091
Clone Name UMAB199
Target Homo sapiens programmed cell death 1 (PD-1/PDCD1)
Host Mouse
Reactivity Human
Tested Application WB, IHC, IF
Dilution WB: 1:500, IHC: 1:800 to 1:1000, IF: 1:100
Immunohistochemical staining of FFPE human tonsil using anti-PD1

Immunohistochemical staining of FFPE human tonsil using anti-PD1 antibody UM800091 at 1:800. Briefly, FFPE tissues were deparaffinized then pretreated with HIER (ACCEL 3 in 1 EDTA Buffer pH8.7). Primary antibody was incubated 30 minutes at room temperature. Detection was achieved with 2-step anti-mouse HRP polymer (GBI-D22) and visualized with DAB chromogen.

Immunohistochemical staining of FFPE 2 using anti-PD1

Immunohistochemical staining of FFPE 2 human lung carcinomas (A & B) and 2 human melanomas (C & D) using anti-PD1 antibody UM800091 at 1:800. Image shows tumor cells are negative for PD-1 while infiltrating lymphocytes showing strong positive PD-1 cells. IHC was done the same as tonsil panel on the left.

Antibodies against commonly studied proteins in Immuno-Oncology

Protein Name Antibody Description SKU
ARG1 ARG1 mouse monoclonal antibody, clone OTI4E6 TA502413
B7-1/CD80 CD80 mouse monoclonal antibody, clone UMAB65 UM500055
B7-H3/CD276 Rabbit Polyclonal CD276 Antibody TA355034
B7-H4/VTCN1 Anti-VTCN1 mouse monoclonal antibody TA810072
CD160 Rabbit Polyclonal Anti-CD160 Antibody TA349762
CD19 CD19 mouse monoclonal antibody, clone UMAB103 UM500071
CD244 Rabbit Polyclonal Anti-CD244 Antibody TA349773
IDO1 IDO1 mouse monoclonal antibody, clone UMAB126 UM500091
IL-10RA IL10RA mouse monoclonal antibody, clone OTI1D10 TA506929
IL-13 IL13 mouse monoclonal antibody, clone OTI6D3 TA804004
IL-4 Rabbit polyclonal IL4 Antibody (C-term) TA324803
LAG3 LAG3 mouse monoclonal antibody, clone OTI8F6 TA807082
MICA Rabbit Polyclonal MICA Antibody TA306443
MICB anti MIC-B monoclonal antibody, cloneB-D55 AM31286AF-N
NCAM1 NCAM1/CD56 mouse monoclonal antibody, clone UMAB194 UM800086
Notch1 Anti-Notch1 mouse monoclonal antibody, clone OTI3E12 TA500078
PTGS1 Anti-PTGS1 Rabbit Polyclonal Antibody TA321247
PD-L1 PD-L1/CD274 mouse monoclonal antibody, clone OTI13G7 TA809809
PD-L2 PDL2/PDCD1LG2 mouse monoclonal antibody, clone OTI5A7 TA808997
SOX2 Rabbit Polyclonal Antibody against SOX2 - Embryonic Stem Cell Marker TA301462
TIM3 HAVCR2 mouse monoclonal antibody, clone OTI2E2 TA807034
Beta Tubulin Rabbit polyclonal anti-TUBB(beta Tubulin) antibody, Loading control TA301569
Vimentin Vimentin (VIM) mouse monoclonal antibody, clone UMAB160 UM800055