TissueScan, Cancer Survey cDNA Array 384 - I

Snippet: MDA-MB-231 cells were transfected with scrambled control siRNA or siRNA against sclerostin (Origene) using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions. For collection of conditioned medium, MDA-MB-231 cells were cultured in the presence of 1% FBS for 24 hours, and conditioned medium was collected and stored at –80°C. Osteoblast differentiation was ind...

Snippet: TissueScan Cancer Survey Panel (OriGene) was used for screening 18 different human cancer types (over 380 biospecimens), and Breast Cancer Panel I-IV (OriGene) was used for determining the expression levels of genes (approximately 150 biospecimens)

Snippet: We analyzed the RT2 Pro ler PCR Arrays (QIAGEN, Hilden, Germany) and the Cancer Survey cDNA array or Breast Cancer cDNA array (OriGene, Rockville, MD, USA) to detect mRNA using qRT- PCR with CYBR Green and a ViiA7 System (Life Technologies, Carlsbad, CA, USA)

Snippet: To examine the gene expression levels for TCRP1 in different cancer patient tissue samples, we performed quantitative PCR analysis on TissueScan Cancer and Normal Tissue cDNA Arrays (Origene Company, Rockville, MD, USA), which include 392 cases from different ... All 3 versions Cite SaveSaving

Snippet: …Measurement of BTK-A and BTK-C mRNA expression in prostate tumor tissues was performed using an array of first strand complementary DNA (cDNA) from human prostate cancer tissues and lymphoma samples contained in the TissueScan Cancer Survey Panel (CSRT302) in 384-well plates from OriGene (Rockville, MD)