GLI2 (NM_005270) Human Over-expression Lysate
GLI2 HEK293T cell transient overexpression lysate (as WB positive control)
Product Datasheet for 'LC417413'
|Description||GLI2 HEK293T cell transient overexpression lysate (as WB positive control)|
|Expression cDNA Clone or AA Sequence||TrueORF Clone RC217291|
|Detection Antibodies||Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)|
|Accession Number||NM_005270, NP_005261|
|Other Names||CJS; HPE9; PHS2; THP1; THP2|
|Predicted MW||167.6 kDa|
|Components||1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate|
|Storage||The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.|
|Preparation||HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS|
|Protein Pathways||Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.