CHKL (CHKB) (NM_152253) Human Mass Spec Standard

SKU
PH322406
CHKB MS Standard C13 and N15-labeled recombinant protein (NP_689466)
  $3,360.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC222406
Predicted MW 13.3 kDa
Protein Sequence
Protein Sequence
>RC222406 representing NM_152253
Red=Cloning site Green=Tags(s)

MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELR
VYPVRWEVRGQPLRCADRGQGSAAGPSGCSMFSPPSCARAWGGAGPAWPGGGRGRGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_689466
RefSeq Size 4914
RefSeq ORF 381
Synonyms CHETK; CHKL; choline/ethanolamine kinase; choline kinase-like; choline kinase beta; CKEKB; EKB
Locus ID 1120
UniProt ID Q9Y259
Cytogenetics 22q13.33
Summary Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. provided by RefSeq, Jun 2009
Protein Families Druggable Genome
Protein Pathways Glycerophospholipid metabolism, Metabolic pathways
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Other "CHKL" proteins (9)
SKU Description Size Price
PH310253 CHKB MS Standard C13 and N15-labeled recombinant protein (NP_005189) 10 ug
$3,360.00
LC407681 Lyophilized CHKB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417454 Lyophilized CHKB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429244 Lyophilized CHKB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY407681 Transient overexpression lysate of choline kinase beta (CHKB), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY417454 Transient overexpression lysate of choline kinase beta (CHKB) (as WB positive control) 100 ug
$436.00
LY429244 Transient overexpression lysate of choline kinase beta (CHKB) (as WB positive control) 100 ug
$436.00
TP310253 Recombinant protein of human choline kinase beta (CHKB), transcript variant 1, 20 µg 20 ug
$867.00
TP322406 Recombinant protein of human choline kinase beta (CHKB), transcript variant 2, 20 µg 20 ug
$867.00

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