Mouse Zswim8 activation kit by CRISPRa

SKU GA215945

Zswim8 CRISPRa kit - CRISPR gene activation of mouse zinc finger SWIM-type containing 8

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Zswim8
• Locus ID: 268721
• Components:

  GA215945G1, Zswim8 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA215945G2, Zswim8 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA215945G3, Zswim8 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001252081, NM_001252082, NM_027996, NM_175544
• UniProt ID: Q3UHH1
• Synonyms: 2310021P13Rik; 4832404P21Rik; Kiaa0913; mKIAA0913
• Summary: Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (By similarity). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (By similarity). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (By similarity).[UniProtKB/Swiss-Prot Function]