Human C19orf46 (SYNE4) activation kit by CRISPRa

SKU GA116082

SYNE4 CRISPRa kit - CRISPR gene activation of human spectrin repeat containing nuclear envelope family member 4

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: SYNE4
• Locus ID: 163183
• Components:

  GA116082G1, C19orf46 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA116082G2, C19orf46 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA116082G3, C19orf46 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001039876, NM_001297735, NM_153233
• UniProt ID: Q8N205
• Synonyms: C19orf46; DFNB76; Nesp4
• Summary: This gene is a member of the nesprin family of genes, that encode KASH (Klarsicht, Anc-1, Syne Homology) domain-containing proteins. In addition to the KASH domain, this protein also contains a coiled-coil and leucine zipper region, a spectrin repeat, and a kinesin-1 binding region. This protein localizes to the outer nuclear membrane, and is part of the linker of nucleoskeleton and cytoskeleton (LINC) complex in the nuclear envelope. LINC complexes are formed by SUN (Sad1, UNC-84)-KASH pairs, and are thought to mechanically couple nuclear components to the cytoskeleton. Mutations in this gene have been associated with progressive high-frequency hearing loss. The absence of this protein in mice also caused hearing loss, and changes in hair cell morphology in the ears. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]