Human SPAAR activation kit by CRISPRa

SKU GA115957

SPAAR CRISPRa kit - CRISPR gene activation of human small regulatory polypeptide of amino acid response

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: SPAAR
• Locus ID: 158376
• Components:

  GA115957G1, SPAAR gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA115957G2, SPAAR gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA115957G3, SPAAR gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NR_024283, NM_001348107
• Summary: Isoform 2: Negative regulator of amino acid sensing and mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels and amino acids (PubMed:28024296). Negatively regulates mTORC1 activation by inhibiting recruitment of mTORC1 to lysosomes upon stimulation with amino acids: acts by promoting the formation of a tightly bound supercomplex composed of the lysosomal V-ATPase, Ragulator and Rag GTPases, preventing recruitment of mTORC1 (PubMed:28024296). Acts as a regulator of muscle regeneration following injury by regulating mTORC1 activation (By similarity).[UniProtKB/Swiss-Prot Function]