Human Tankyrase 2 (TNKS2) activation kit by CRISPRa

SKU GA112943

TNKS2 CRISPRa kit - CRISPR gene activation of human tankyrase 2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: TNKS2
• Locus ID: 80351
• Components:

  GA112943G1, Tankyrase 2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA112943G2, Tankyrase 2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA112943G3, Tankyrase 2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025235
• UniProt ID: Q9H2K2
• Synonyms: ARTD6; PARP-5b; PARP-5c; PARP5B; PARP5C; pART6; TANK2; TNKL
• Summary: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:11739745, PubMed:11802774, PubMed:19759537, PubMed:21478859, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Stimulates 26S proteasome activity (PubMed:23622245).[UniProtKB/Swiss-Prot Function]