Human PION (GSAP) activation kit by CRISPRa

SKU GA110062

GSAP CRISPRa kit - CRISPR gene activation of human gamma-secretase activating protein

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: GSAP
• Locus ID: 54103
• Components:

  GA110062G1, PION gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA110062G2, PION gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA110062G3, PION gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017439, NM_001350896, NM_001350897, NM_001350898, NM_001350899, NM_001350900, NM_001350901, NR_146938, NR_146937
• UniProt ID: A4D1B5
• Synonyms: PION
• Summary: Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).[supplied by OMIM, Nov 2010]