DDIT3 (NM_004083) Human Untagged Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | DDIT3 |
| Synonyms | AltDDIT3; C/EBPzeta; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_004083 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004083.4, NP_004074.2 |
| RefSeq Size | 927 bp |
| RefSeq ORF | 510 bp |
| Locus ID | 1649 |
| UniProt ID | P35638 |
| Cytogenetics | 12q13.3 |
| Domains | BRLZ |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | MAPK signaling pathway |
| Summary | This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. provided by RefSeq, Aug 2010 Transcript Variant: This variant (5) lacks an internal segment in the 5' region, resulting in a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, as compared to isoform 1. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004083" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201301 | DDIT3 (Myc-DDK-tagged)-Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5 | 10 ug |
$300.00
|
|
| RC201301L1 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201301L2 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5, mGFP tagged | 10 ug |
$600.00
|
|
| RC201301L3 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201301L4 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5, mGFP tagged | 10 ug |
$600.00
|
|
| RG201301 | DDIT3 (tGFP-tagged) - Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5 | 10 ug |
$489.00
$500.00
|
|
| SC117581 | DDIT3 (untagged)-Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 5 | 10 ug |
$300.00
|
|
Citations
Endoplasmic reticulum stress induced by tunicamycin increases resistin messenger ribonucleic acid through the pancreatic endoplasmic reticulum eukaryotic initiation factor 2α kinase-activating transcription factor 4-CAAT/enhancer binding protein-α homologous protein pathway in THP-1 human monocytes
Hamada, Onuma, Ochi et al
J Diabetes Investig (2016) 7 (3), 312-23 DOI: 10.1111/jdi.12434
Hamada, Onuma, Ochi et al
J Diabetes Investig (2016) 7 (3), 312-23 DOI: 10.1111/jdi.12434
Cited by 9
AI Summary: In this study, OriGene’s human CHOP (SR304225) and ATF4 (SR321091) siRNA products were used to knock down gene expression, helping to demonstrate that tunicamycin-induced endoplasmic reticulum stress increases resistin mRNA in THP-1 monocytes via the PERK-ATF4-CHOP pathway.
Mechanistic Elucidation of the Antitumor Properties of Withaferin A in Breast Cancer
Nagalingam, Kuppusamy, Singh et al
Cancer Res. (2014)
Nagalingam, Kuppusamy, Singh et al
Cancer Res. (2014)
AI Summary: OriGene contributed to this study by providing the CHOP overexpression construct, which was used to investigate the role of CHOP in the antitumor mechanism of Withaferin A in breast cancer.
Snippet: Molecular Pharmacology, San Francisco, CA;ref. 21). U0126 and trichostatin A (TSA) were procured from Sigma. CHOP overexpression construct was purchased from OriGene Technologies, Inc. Wild-type and S383A-mutant Elk1 ... Cite SaveSaving
C/EBP homologous protein drives pro-catabolic responses in chondrocytes
Husa, Petursson, Lotz et al
Arthritis Res. Ther. (2013) 15 (6), R218 DOI: 10.1186/ar4415
Husa, Petursson, Lotz et al
Arthritis Res. Ther. (2013) 15 (6), R218 DOI: 10.1186/ar4415
Cited by 70
AI Summary: OriGene’s pCMV6-CHOP plasmid was used to overexpress CHOP in human chondrocytes, enabling the study of how increased CHOP levels influence pro-catabolic and apoptotic responses in these cells.
Snippet: Normal cultured human knee articular chondrocytes (passage 1) were transfected with siRNAs of CHOP, GRP78 or XBP1 and the non-target controls, or transfected with CHOP driven by the CMV promoter in pCMV6-CHOP (Origene), or the vector plasmid control, using ... Cite SaveSaving
Suppression of PP2A is critical for protection of melanoma cells upon endoplasmic reticulum stress
Tay, Jin, Tseng et al
Cell Death Dis (2012) 3, e337 DOI: 10.1038/cddis.2012.79
Tay, Jin, Tseng et al
Cell Death Dis (2012) 3, e337 DOI: 10.1038/cddis.2012.79
Cited by 34
AI Summary: OriGene products pCMV6-XL4-Bim EL, pCMV6-AC-CHOP, and pCMV6-AC-PP2A-C plasmid vectors were used for gene overexpression experiments to investigate the roles of Bim, CHOP, and PP2A in melanoma cell responses to endoplasmic reticulum stress.
Snippet: RM.CHOP). Plasmid vectors and transfection. The pCMV6-XL4-Bim EL (SC306048), pCMV6-AC-CHOP (SC324377-20), and pCMV6-AC-PP2A-C (SC321401) were purchased from Origene (Rockville, MD, USA). Cells were
Endoplasmic reticulum stress induced by tunicamycin increases resistin mRNA through the PERK‐ATF4‐CHOP pathway in THP‐1 human monocytes
Hamada, Onuma, Ochi et al
Journal of Diabetes Investigation (2015)
Hamada, Onuma, Ochi et al
Journal of Diabetes Investigation (2015)
Showing 1-5 of 5 papers.
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