Twist (TWIST1) (NM_000474) Human Untagged Clone
TWIST1 (untagged)-Human twist homolog 1 (Drosophila) (TWIST1)
|Product Name||Twist (TWIST1) (NM_000474) Human Untagged Clone|
|Type||Human Untagged Clone|
|Synonyms||ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST|
|E. coli Selection||Ampicillin (100 ug/mL)|
>OriGene sequence for NM_000474.3 GAGGTATAAGAGCCTCCAAGTCTGCAGCTCTCGCCCAACTCCCAGACACCTCGCGGGCTC
>OriGene 5' read for NM_000474.3 unedited
|Restriction Sites||Please inquire|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.|
|Product Components||The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1669 bp|
|RefSeq ORF||609 bp|
|Protein Families||Druggable Genome|
|Gene Summary||This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]|
|The use of this cDNA Clones has been cited in the following citations:|
EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis
,Ihira, K;Dong, P;Xiong, Y;Watari, H;Konno, Y;Hanley, SJ;Noguchi, M;Hirata, N;Suizu, F;Yamada, T;Kudo, M;Sakuragi, N;,
,PubMed ID 28088786
Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes
,Meier, D;Lopez, M;Franken, P;Fontana, A;,
PLoS ONE Sep 2015
,PubMed ID 26361389
Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation
,Lee, S;Craig, BT;Romain, CV;Qiao, J;Chung, DH;,
Cancer Lett. Sep 2014
,PubMed ID 25264923
|cDNA Clone Resources|
|RC202920||TWIST1 (Myc-DDK-tagged)-Human twist homolog 1 (Drosophila) (TWIST1)||
|RG202920||TWIST1 (GFP-tagged) - Human twist homolog 1 (Drosophila) (TWIST1)||
|RC202920L1||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged||
|RC202920L2||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged||
|RC202920L3||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged||
|RC202920L4||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged||