ABCG2 (NM_004827) Human Untagged Clone

CAT#: SC320948

ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCG2
• Synonyms: ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004827.2 GTCAGCGCTGCCTGAGCTCGTCCCCTGGATGTCCGGGTCTCCCCAGGCGGCCACCCGCCG
  GCTCCCATCGTGACCTCCAGCCGCAGCGCCTCCCACGCCGGCCGCCGCGCGAGGGGAGCG
  CTCGGGCGCGCCGGGTGTGGTTGGGGGAAGGGGTTGTGCCGCGCGCGGGCTGCGTGCTGT
  GCCCACTCAAAAGGTTCCGGGCGCGCAGGAGGGAAGAGGCAGTGCCCGCCACTCCCACTG
  AGATTGAGAGACGCGGCAAGGAGGCAGCCTGTGGAGGAACTGGGTAGGATTTAGGAATGC
  ACCGTGCACATGCTTGGTGGTCTTGTTAAGTGGAAACTGCTGCTTTAGAGTTTGTTTGGA
  AGGTCCGGGTGACTCATCCCAACATTTACATCCTTAATTGTTAAAGCGCTGCCTCCGAGC
  GCACGCATCCTGAGATCCTGAGCCTTTGGTTAAGACCGAGCTCTATTAAGCTGAAAAGAT
  AAAAACTCTCCAGATGTCTTCCAGTAATGTCGAAGTTTTTATCCCAGTGTCACAAGGAAA
  CACCAATGGCTTCCCCGCGACAGCTTCCAATGACCTGAAGGCATTTACTGAAGGAGCTGT
  GTTAAGTTTTCATAACATCTGCTATCGAGTAAAACTGAAGAGTGGCTTTCTACCTTGTCG
  AAAACCAGTTGAGAAAGAAATATTATCGAATATCAATGGGATCATGAAACCTGGTCTCAA
  CGCCATCCTGGGACCCACAGGTGGAGGCAAATCTTCGTTATTAGATGTCTTAGCTGCAAG
  GAAAGATCCAAGTGGATTATCTGGAGATGTTCTGATAAATGGAGCACCGCGACCTGCCAA
  TTTCAAATGTAATTCAGGTTACGTGGTACAAGATGATGTTGTGATGGGCACTCTGACGGT
  GAGAGAAAACTTACAGTTCTCAGCAGCTCTTCGGCTTGCAACAACTATGACGAATCATGA
  AAAAAACGAACGGATTAACAGGGTCATTCAAGAGTTAGGTCTGGATAAAGTGGCAGACTC
  CAAGGTTGGAACTCAGTTTATCCGTGGTGTGTCTGGAGGAGAAAGAAAAAGGACTAGTAT
  AGGAATGGAGCTTATCACTGATCCTTCCATCTTGTTCTTGGATGAGCCTACAACTGGCTT
  AGACTCAAGCACAGCAAATGCTGTCCTTTTGCTCCTGAAAAGGATGTCTAAGCAGGGACG
  AACAATCATCTTCTCCATTCATCAGCCTCGATATTCCATCTTCAAGTTGTTTGATAGCCT
  CACCTTATTGGCCTCAGGAAGACTTATGTTCCACGGGCCTGCTCAGGAGGCCTTGGGATA
  CTTTGAATCAGCTGGTTATCACTGTGAGGCCTATAATAACCCTGCAGACTTCTTCTTGGA
  CATCATTAATGGAGATTCCACTGCTGTGGCATTAAACAGAGAAGAAGACTTTAAAGCCAC
  AGAGATCATAGAGCCTTCCAAGCAGGATAAGCCACTCATAGAAAAATTAGCGGAGATTTA
  TGTCAACTCCTCCTTCTACAAAGAGACAAAAGCTGAATTACATCAACTTTCCGGGGGTGA
  GAAGAAGAAGAAGATCACAGTCTTCAAGGAGATCAGCTACACCACCTCCTTCTGTCATCA
  ACTCAGATGGGTTTCCAAGCGTTCATTCAAAAACTTGCTGGGTAATCCCCAGGCCTCTAT
  AGCTCAGATCATTGTCACAGTCGTACTGGGACTGGTTATAGGTGCCATTTACTTTGGGCT
  AAAAAATGATTCTACTGGAATCCAGAACAGAGCTGGGGTTCTCTTCTTCCTGACGACCAA
  CCAGTGTTTCAGCAGTGTTTCAGCCGTGGAACTCTTTGTGGTAGAGAAGAAGCTCTTCAT
  ACATGAATACATCAGCGGATACTACAGAGTGTCATCTTATTTCCTTGGAAAACTGTTATC
  TGATTTATTACCCATGAGGATGTTACCAAGTATTATATTTACCTGTATAGTGTACTTCAT
  GTTAGGATTGAAGCCAAAGGCAGATGCCTTCTTCGTTATGATGTTTACCCTTATGATGGT
  GGCTTATTCAGCCAGTTCCATGGCACTGGCCATAGCAGCAGGTCAGAGTGTGGTTTCTGT
  AGCAACACTTCTCATGACCATCTGTTTTGTGTTTATGATGATTTTTTCAGGTCTGTTGGT
  CAATCTCACAACCATTGCATCTTGGCTGTCATGGCTTCAGTACTTCAGCATTCCACGATA
  TGGATTTACGGCTTTGCAGCATAATGAATTTTTGGGACAAAACTTCTGCCCAGGACTCAA
  TGCAACAGGAAACAATCCTTGTAACTATGCAACATGTACTGGCGAAGAATATTTGGTAAA
  GCAGGGCATCGATCTCTCACCCTGGGGCTTGTGGAAGAATCACGTGGCCTTGGCTTGTAT
  GATTGTTATTTTCCTCACAATTGCCTACCTGAAATTGTTATTTCTTAAAAAATATTCTTA
  AATTTCCCCTTAATTCAGTATGATTTATCCTCACATAAAAAAGAAGCACTTTGATTGAAG
  TATTCAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004827
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004827.2, NP_004818.2
• RefSeq Size: 4445 bp
• RefSeq ORF: 1968 bp
• Locus ID: 9429
• UniProt ID: Q9UNQ0
• Cytogenetics: 4q22.1
• Domains: ABC_tran, AAA
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1).