PUS10 (NM_144709) Human Tagged ORF Clone

SKU RG210979

PUS10 (tGFP-tagged) - Human pseudouridylate synthase 10 (PUS10)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PUS10
• Synonyms: CCDC139; DOBI; Hup10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210979 representing NM_144709; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCAGTTGTTGCTCAATACTGGTACTTGTCCAAGAT; GTATCTTCAGATTCTGTGGTGTGGATTTTCATGCACCTTACAAACTTCCATACAAGGAGTTGCTCAATGA; ACTACAGAAATTTCTGGAAACTGAAAAAGATGAATTAATTTTGGAAGTTATGAACCCACCTCCCAAGAAA; ATTCGACTGCAAGAACTGGAAGATAGTATTGATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGTTA; GTCATGTTGGAAGCACTGCTTCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCTAGGAATTCTTCA; AGAATTCTGTGAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGAGGCCTCTGGGTTTGAATTCACC; AGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATCTGTAAGAGAGCATGCTGCATGGTTGCTGGTAA; AACAGGAAATGGGAAAGCAGAGTCTGTCGCTGGGAAGAGATGATATAGTTCAGCTAAAAGAAGCCTACAA; ATGGATAACTCACCCCCTGTTTTCAGAGGAACTGGGTGTTCCCATTGATGGAAAGAGCTTGTTTGAAGTG; AGTGTGGTCTTTGCTCACCCAGAAACAGTTGAGGATTGCCACTTCCTAGCTGCGATATGCCCAGATTGTT; TTAAGCCAGCCAAAAACAAACAGTCTGTATTCACTAGAATGGCAGTTATGAAAGCCTTGAATAAGATAAA; GGAAGAGGATTTCCTTAAGCAGTTTCCTTGTCCTCCAAACTCACCAAAGGCTGTATGCGCTGTTCTTGAA; ATTGAATGTGCTCATGGTGCTGTTTTTGTGGCTGGGAGATATAATAAATACTCCAGGAATCTACCACAAA; CTCCTTGGATAATTGATGGAGAAAGGAAGCTGGAATCTTCAGTGGAAGAATTAATTTCAGATCATCTGTT; GGCAGTATTTAAAGCAGAGAGTTTTAATTTTTCATCCTCTGGAAGAGAAGATGTAGATGTGAGAACATTA; GGAAATGGAAGGCCCTTTGCAATTGAGCTGGTGAATCCTCATAGAGTACATTTCACTTCACAAGAAATTA; AGGAACTTCAGCAGAAAATTAATAACTCATCTAACAAAATCCAAGTACGTGACTTGCAGCTTGTCACAAG; AGAGGCAATAGGACATATGAAAGAAGGTGAAGAAGAAAAGACAAAGACCTACAGTGCCTTAATTTGGACA; AATAAAGCGATACAGAAGAAAGACATTGAATTCCTAAATGACATAAAGGACTTAAAAATCGACCAGAAAA; CACCTTTGCGCGTCCTTCACCGAAGGCCCCTGGCTGTGCGAGCTCGCGTCATTCACTTCATGGAGACACA; GTACGTGGATGAGCACCACTTCCGCCTCCACTTGAAAACTCAGGCTGGCACCTACATTAAAGAGTTTGTA; CATGGAGACTTCGGGAGAACCAAGCCAAACATTGGGTCCCTGATGAATGTGACTGCAGACATTCTGGAGC; TGGATGTTGAGTCTGTAGATGTTGACTGGCCACCTGCTCTGGATGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210979 representing NM_144709; Red=Cloning site Green=Tags(s); ; MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKK; IRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFT; SLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEV; SVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLE; IECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTL; GNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWT; NKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFV; HGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144709
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144709.4
• RefSeq Size: 2097 bp
• RefSeq ORF: 1590 bp
• Locus ID: 150962
• UniProt ID: Q3MIT2
• Cytogenetics: 2p16.1-p15
• Summary: Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]