Human PUS10 (NM_144709) AAV Particle

SKU RC210979A1V

AAV ORF Particles, serotype AAV-2, PUS10 (Myc-DDK-tagged)-Human pseudouridylate synthase 10 (PUS10), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: PUS10
• Synonyms: CCDC139; DOBI; Hup10
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210979 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCAGTTGTTGCTCAATACTGGTACTTGTCCAAGAT; GTATCTTCAGATTCTGTGGTGTGGATTTTCATGCACCTTACAAACTTCCATACAAGGAGTTGCTCAATGA; ACTACAGAAATTTCTGGAAACTGAAAAAGATGAATTAATTTTGGAAGTTATGAACCCACCTCCCAAGAAA; ATTCGACTGCAAGAACTGGAAGATAGTATTGATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGTTA; GTCATGTTGGAAGCACTGCTTCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCTAGGAATTCTTCA; AGAATTCTGTGAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGAGGCCTCTGGGTTTGAATTCACC; AGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATCTGTAAGAGAGCATGCTGCATGGTTGCTGGTAA; AACAGGAAATGGGAAAGCAGAGTCTGTCGCTGGGAAGAGATGATATAGTTCAGCTAAAAGAAGCCTACAA; ATGGATAACTCACCCCCTGTTTTCAGAGGAACTGGGTGTTCCCATTGATGGAAAGAGCTTGTTTGAAGTG; AGTGTGGTCTTTGCTCACCCAGAAACAGTTGAGGATTGCCACTTCCTAGCTGCGATATGCCCAGATTGTT; TTAAGCCAGCCAAAAACAAACAGTCTGTATTCACTAGAATGGCAGTTATGAAAGCCTTGAATAAGATAAA; GGAAGAGGATTTCCTTAAGCAGTTTCCTTGTCCTCCAAACTCACCAAAGGCTGTATGCGCTGTTCTTGAA; ATTGAATGTGCTCATGGTGCTGTTTTTGTGGCTGGGAGATATAATAAATACTCCAGGAATCTACCACAAA; CTCCTTGGATAATTGATGGAGAAAGGAAGCTGGAATCTTCAGTGGAAGAATTAATTTCAGATCATCTGTT; GGCAGTATTTAAAGCAGAGAGTTTTAATTTTTCATCCTCTGGAAGAGAAGATGTAGATGTGAGAACATTA; GGAAATGGAAGGCCCTTTGCAATTGAGCTGGTGAATCCTCATAGAGTACATTTCACTTCACAAGAAATTA; AGGAACTTCAGCAGAAAATTAATAACTCATCTAACAAAATCCAAGTACGTGACTTGCAGCTTGTCACAAG; AGAGGCAATAGGACATATGAAAGAAGGTGAAGAAGAAAAGACAAAGACCTACAGTGCCTTAATTTGGACA; AATAAAGCGATACAGAAGAAAGACATTGAATTCCTAAATGACATAAAGGACTTAAAAATCGACCAGAAAA; CACCTTTGCGCGTCCTTCACCGAAGGCCCCTGGCTGTGCGAGCTCGCGTCATTCACTTCATGGAGACACA; GTACGTGGATGAGCACCACTTCCGCCTCCACTTGAAAACTCAGGCTGGCACCTACATTAAAGAGTTTGTA; CATGGAGACTTCGGGAGAACCAAGCCAAACATTGGGTCCCTGATGAATGTGACTGCAGACATTCTGGAGC; TGGATGTTGAGTCTGTAGATGTTGACTGGCCACCTGCTCTGGATGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210979 protein sequence; Red=Cloning site Green=Tags(s); ; MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKK; IRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFT; SLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEV; SVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLE; IECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTL; GNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWT; NKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFV; HGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_144709
• ORF Size: 1587 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_144709.1
• RefSeq Size: 3820 bp
• RefSeq ORF: 1590 bp
• Locus ID: 150962
• UniProt ID: Q3MIT2
• Cytogenetics: 2p16.1-p15
• MW: 60.2 kDa