PUS10 (NM_144709) Human Untagged Clone

SKU SC120726

PUS10 (untagged)-Human pseudouridylate synthase 10 (PUS10)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PUS10
• Synonyms: CCDC139; DOBI; Hup10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120726 sequence for NM_144709 edited (data generated by NextGen Sequencing); ATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCAGTTGTTGCTCAATACTGGTACT; TGTCCAAGATGTATCTTCAGATTCTGTGGTGTGGATTTTCATGCACCTTACAAACTTCCA; TACAAGGAGTTGCTCAATGAACTACAGAAATTTCTGGAAACTGAAAAAGATGAATTAATT; TTGGAAGTTATGAACCCACCTCCCAAGAAAATTCGACTGCAAGAACTGGAAGATAGTATT; GATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGTTAGTCATGTTGGAAGCACTGCT; TCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCTAGGAATTCTTCAAGAATTCTGT; GAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGAGGCCTCTGGGTTTGAATTCACC; AGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATCTGTAAGAGAGCATGCTGCATGG; TTGCTGGTAAAACAGGAAATGGGAAAGCAGAGTCTGTCGCTGGGAAGAGATGATATAGTT; CAGCTAAAAGAAGCCTACAAATGGATAACTCACCCCCTGTTTTCAGAGGAACTGGGTGTT; CCCATTGATGGAAAGAGCTTGTTTGAAGTGAGTGTGGTCTTTGCTCACCCAGAAACAGTT; GAGGATTGCCACTTCCTAGCTGCGATATGCCCAGATTGTTTTAAGCCAGCCAAAAACAAA; CAGTCTGTATTCACTAGAATGGCAGTTATGAAAGCCTTGAATAAGATAAAGGAAGAGGAT; TTCCTTAAGCAGTTTCCTTGTCCTCCAAACTCACCAAAGGCTGTATGCGCTGTTCTTGAA; ATTGAATGTGCTCATGGTGCTGTTTTTGTGGCTGGGAGATATAATAAATACTCCAGGAAT; CTACCACAAACTCCTTGGATAATTGATGGAGAAAGGAAGCTGGAATCTTCAGTGGAAGAA; TTAATTTCAGATCATCTGTTGGCAGTATTTAAAGCAGAGAGTTTTAATTTTTCATCCTCT; GGAAGAGAAGATGTAGATGTGAGAACATTAGGAAATGGAAGGCCCTTTGCAATTGAGCTG; GTGAATCCTCATAGAGTACATTTCACTTCACAAGAAATTAAGGAACTTCAGCAGAAAATT; AATAACTCATCTAACAAAATCCAAGTACGTGACTTGCAGCTTGTCACAAGAGAGGCAATA; GGACATATGAAAGAAGGTGAAGAAGAAAAGACAAAGACCTACAGTGCCTTAATTTGGACA; AATAAAGCGATACAGAAGAAAGACATTGAATTCCTAAATGACATAAAGGACTTAAAGATC; GACCAGAAAACACCTTTGCGCGTCCTTCACCGAAGGCCCCTGGCTGTGCGAGCTCGCGTC; ATTCACTTCATGGAGACACAGTACGTGGATGAGCACCACTTCCGCCTCCACTTGAAAACT; CAGGCTGGCACCTACATTAAAGAGTTTGTACATGGAGACTTCGGGAGAACCAAGCCAAAC; ATTGGGTCCCTGATGAATGTGACTGCAGACATTCTGGAGCTGGATGTTGAGTCTGTAGAT; GTTGACTGGCCACCTGCTCTGGATGACTAG; ; Clone variation with respect to NM_144709.2; 1317 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144709 unedited; ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTGTTAGCAA; AGGAGGGTTATAATTATTCAATATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCA; GTTGTTGCTCAATACTGGTACTTGTCCAAGATGTATCTTCAGATTCTGTGGTGTGGATTT; TCATGCACCTTACAAACTTCCATACAAGGAGTTGCTCAATGAACTACAGAAATTTCTGGA; AACTGAAAAAGATGAATTAATTTTGGAAGTTATGAACCCACCTCCCAAGAAAATTCGACT; GCAAGAACTGGAAGATAGTATTGATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGT; TAGTCATGTTGGAAGCACTGCTTCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCT; AGGAATTCTTCAAGAATTCTGTGAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGA; GGCCTCTGGGTTTGAATTCACCAGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATC; TGTAAGAGAGCATGCTGCATGGTTGCTGGTAAAACAGGAAATGGGAAAGCAGAGTCTGTC; GCTGGGAAGAGATGATATAGTTCAGCTAAAAGAAGCCTACAAATGGATAACTCACCCCCT; GTTTTCAGAGGAACTGGGTGTTCCCATTGATGGAAAGAGCTTGTTTGAAGTGAGTGTGGT; CTTTGCTCACCCAGAAAACAGTTGAGATTGCCACTTCCTAGCTGCGATATGCCCAGATTG; TTNTAAGCCAGCCAAAAACAACAGTCTGTATTCACTAGAATGGCACGTATGAAAGCCCTG; TATAAGATAAAGAAGAAGATTNCCTTAGCAGTTTCCTGTCCTCCAACTCACAAAGCTGTT; GCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_144709 unedited; GTACACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTT; AACTATATAAGATTGTATTAAATAGTCTTGGGCTATTTTTTGGTAAGCTCTTTGATTTGG; TAACTACATTCCAAAAGTTAATTATCAGTTACATGAGTTTAAATGGCATCCATAGCTGCC; CCTTCACACAAACAGGTGCTCAGTAATAGGGCTTATTAGAAAGACCCTAGAAACCAAGAA; ACTTCAGTTCTAGTCCTCAATATAGAAGTAGCTAACAATTTTGGATAAGTCATTTAACTT; CTAGTCTTTGGTTTCCATATTTATAAAATGGAGACAATATCCTATCTACTTTAATAAGGT; ATTATTAAAATGTTTATAATTGGATAATATATTGAAGCATTCTGAAATAATACCAGTTCG; TCTTTGTTTAGCAATCTCAATTTTTCAAATTCTTAACCCTGGGATTGAAGTTATTAAAGG; CACTTGTTTCTCCTTATTTAAAAGATTTATGAAAGGCTCCTACCATGGCATGCCTTGGCT; CCTACGGTTTTACTTTCCCTCCACAGGAAACCTATATTGACAATTTTAGATTGTCAAAAT; TCTTTCTTAATGAAAGTATTAATGAGAATTATTTCATTATTCATAGTTTGGTTAAGATTT; ATGACAAATCACAGCCAAAAGAACTTCATTGGTAATAGCTTGTTTGAGGCTTCCTGGGGA; AAATAATGATGTCAGATTTGCATTTGCAATCTTAGACTATTATAGGATATTATGCTGCAT; AAATCTAGATATAACTTTTATTCAGATAATTTACAAAAATTAGAACATGATTTTAAAGGT; GGTATATCTCTTGTGGAGGGAAATCTTGCTGAAATACAGCTTCTTAGGTTACAGAGAATG; TGTCCTGTTTGGCACCTCTCTAATGAAAGAATTANGAGAATATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_144709
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144709.1, NP_653310.1
• RefSeq Size: 2097 bp
• RefSeq ORF: 1590 bp
• Locus ID: 150962
• UniProt ID: Q3MIT2
• Cytogenetics: 2p16.1-p15
• Summary: Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]