DHX8 (NM_004941) Human Tagged ORF Clone

SKU RG208403

DHX8 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DHX8
• Synonyms: DDX8; Dhr2; HRH1; PRP22; PRPF22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208403 representing NM_004941; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTTGCCA; AACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTGGGGATCAACGACAA; GGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACCTTTGATACTTTTAAGGCTTCTCTC; GTCAAAAATGGTGCAGAATTTACGGATTCTCTTATTAGTAACTTGCTGCGTCTCATACAAACCATGCGGC; CTCCAGCGAAGCCTTCCACTAGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGA; ACTCTTCCCAGTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAA; GTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAGCAAAGAG; ATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGAAACCGAGATCGAGA; CAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACACCGATCCCGCTCTCGATCACGTTCC; AGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATATCGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAAG; ACCGGAAGGACCGGGACAAATATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCATGTGGACCGCCC; TCCTCCAGAAGAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATGCAGTTTGGTTGC; TTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGGCGGGAGG; GTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAAGTCAAAGTGCTGTCCTTCAC; TGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAAGAGACTGGAGAAGATCTAAACCCAAATAGA; CGGCGAAATCTTGTCGGGGAGACCAATGAGGAGACCTCAATGCGGAATCCTGATAGACCCACTCACTTGT; CCCTTGTCAGTGCTCCTGAAGTAGAGGACGACTCACTGGAACGCAAGCGCCTCACCCAAATCTCTGACCC; AGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAAGAATTTCCAGACTTTGAT; GAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTTGAGATTGAATTGGTTGAGG; AAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGCCCCATTAAAATTGTCAAGAA; CCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGTGCCTTGGCCAAAGAAAGGCGGGAACTCAAA; CAGGCCCAGCGGGAAGCTGAGATGGATTCTATTCCCATGGGACTCAACAAACACTGGGTTGACCCTCTGC; CTGATGCGGAAGGCAGACAGATTGCTGCCAACATGAGGGGTATTGGGATGATGCCCAATGATATTCCTGA; GTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAAAAGACCCAGATGTCAATCCTTGAG; CAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTCCAGGCCGTCCATGACAATCAGATCC; TGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATCACCCAGTACCTGGCGGAGGCAGGCTA; CACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCGGTGGCCAAAAGAGTG; TCAGAGGAGTTTGGTTGTTGCTTAGGCCAAGAGGTGGGCTACACCATTCGATTTGAGGACTGCACTAGCC; CTGAAACAGTCATCAAGTACATGACAGATGGGATGTTGCTTAGAGAGTGCTTGATTGACCCTGACCTCAC; TCAGTACGCGATCATCATGTTGGACGAGGCACATGAGAGGACAATTCACACTGATGTGCTCTTTGGATTG; TTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTGATTGTCACCTCAGCCACCTTGGATGCAGTGA; AGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACCATCCCAGGTCGAACATATCCAGTGGAAATACT; GTACACAAAGGAACCTGAGACAGATTATCTGGATGCCAGCCTGATTACTGTTATGCAGATTCATTTAACA; GAACCACCAGGTGATATCCTGGTCTTCCTGACTGGTCAGGAAGAAATTGATACTGCTTGTGAGATCCTGT; ATGAAAGAATGAAATCCCTGGGACCTGATGTTCCAGAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCC; CAGTGAGATGCAGACCCGAATCTTTGACCCAGCTCCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAAT; ATCGCAGAGACATCGCTGACTATTGATGGTATCTACTATGTGGTGGACCCAGGATTCGTGAAACAGAAAG; TTTACAATTCCAAGACAGGGATTGACCAGCTCGTGGTGACGCCTATTTCTCAGGCTCAGGCAAAGCAACG; AGCTGGCAGAGCTGGGAGAACAGGCCCAGGGAAGTGTTACAGGTTGTACACAGAACGTGCCTACCGAGAT; GAAATGCTGACCACCAACGTGCCGGAAATCCAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGG; CCATGGGTATCAATGATCTGCTGTCCTTTGATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGC; CATGGAGCAGCTGTACACACTGGGGGCCCTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATG; GCAGAGTTCCCTCTGGAGCCAATGCTATGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGTGAGG; AAATGCTGACCATTGTATCCATGCTGTCTGTGCAGAACGTCTTCTATAGGCCCAAGGATAAACAAGCCCT; TGCAGATCAGAAGAAGGCCAAATTCCACCAGACTGAAGGGGACCACCTCACCCTGCTAGCTGTGTACAAC; TCCTGGAAGAACAACAAGTTCTCCAACCCATGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCC; GGGCCCAGGACATTCGCGAGCAGATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGG; CAAGTCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCG; CAGGAGGGTTACCGGACACTGATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCCCTCTTCAACA; GACAGCCAGAATGGGTGGTGTACCATGAGCTGGTGCTCACCACCAAGGAATACATGCGTGAAGTTACCAC; CATCGACCCTCGGTGGCTTGTGGAGTTTGCCCCAGCCTTCTTCAAGGTCTCAGACCCAACTAAGCTAAGC; AAACAGAAGAAGCAACAGCGTCTTGAACCCTTGTACAACCGCTATGAGGAACCCAATGCCTGGAGAATAT; CTCGAGCTTTCCGACGGCGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208403 representing NM_004941; Red=Cloning site Green=Tags(s); ; MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASL; VKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVK; VAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRS; RTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGC; FVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNR; RRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTQISDPEKWEIKQMIAANVLSKEEFPDFD; EETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELK; QAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILE; QRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRV; SEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGL; LKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLT; EPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATN; IAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRD; EMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRM; AEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYN; SWKNNKFSNPWCYENFIQARSLRRAQDIREQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDP; QEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLS; KQKKQQRLEPLYNRYEEPNAWRISRAFRRR; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004941
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004941.1, NP_004932.1
• RefSeq Size: 4201 bp
• RefSeq ORF: 3663 bp
• Locus ID: 1659
• UniProt ID: Q14562
• Cytogenetics: 17q21.31
• Domains: DEAD, HA2, helicase_C, S1
• Protein Pathways: Spliceosome
• Summary: This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]