DHX8 (NM_004941) Human Untagged Clone

SKU SC117043

DHX8 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DHX8
• Synonyms: DDX8; Dhr2; HRH1; PRP22; PRPF22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117043 sequence for NM_004941 edited (data generated by NextGen Sequencing); ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAA; GAACTTGCCAAACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAAT; CACTTGGGGATCAACGACAAGGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAAT; ACCACCTTTGATACTTTTAAGGCTTCTCTCGTCAAAAATGGTGCAGAATTTACGGATTCT; CTTATTAGTAACTTGCTGCGTCTCATACAAACCATGCGGCCTCCAGCGAAGCCTTCCACT; AGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGAACTCTTCCCA; GTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAA; GTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAG; AAGCAAAGAGATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGA; GATCGAAACCGAGATCGAGACAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGG; AGACACCGATCCCGCTCTCGATCACGTTCCAGGACCCGGGAGAGGAATAAAGTGAAGTCT; AGATATCGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAAGACCGGAAGGACCGGGACAAA; TATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCATGTGGACCGCCCTCCTCCAGAA; GAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATGCAGTTTGGTTGC; TTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTC; CGGCGGGAGGGTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAA; GTCAAAGTGCTGTCCTTCACTGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAA; GAGACTGGAGAAGATCTAAACCCAAATAGACGGCGAAATCTTGTCGGGGAGACCAATGAG; GAGACCTCAATGCGGAATCCTGATAGACCCACTCACTTGTCCCTTGTCAGTGCTCCTGAA; GTAGAGGACGACTCACTGGAACGCAAGCGCCTCACCCGAATCTCTGACCCAGAGAAGTGG; GAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAAGAATTTCCAGACTTTGAT; GAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTTGAGATTGAA; TTGGTTGAGGAAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGC; CCCATTAAAATTGTCAAGAACCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGT; GCCTTGGCCAAAGAAAGGCGGGAACTCAAACAGGCCCAGCGGGAAGCTGAGATGGATTCT; ATTCCCATGGGACTCAACAAACACTGGGTTGACCCTCTGCCTGATGCGGAAGGCAGACAG; ATTGCTGCCAACATGAGGGGTATTGGGATGATGCCCAATGATATTCCTGAGTGGAAGAAG; CATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAAAAGACCCAGATGTCAATCCTTGAG; CAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTCCAGGCCGTCCATGAC; AATCAGATCCTGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATCACCCAG; TACCTGGCGGAGGCAGGCTACACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGA; GTGGCAGCTATGTCGGTGGCCAAAAGAGTGTCAGAGGAGTTTGGTTGTTGCTTAGGCCAA; GAGGTGGGCTACACCATTCGATTTGAGGACTGCACTAGCCCTGAAACAGTCATCAAGTAC; ATGACAGATGGGATGTTGCTTAGAGAGTGCTTGATTGACCCTGACCTCACTCAGTACGCG; ATCATCATGTTGGACGAGGCACATGAGAGGACAATTCACACTGATGTGCTCTTTGGATTG; TTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTGATTGTCACCTCAGCCACCTTG; GATGCAGTGAAGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACCATCCCAGGTCGA; ACATATCCAGTGGAAATACTGTACACAAAGGAACCTGAGACAGATTATCTGGATGCCAGC; CTGATTACTGTTATGCAGATTCATTTAACAGAACCACCAGGTGATATCCTGGTCTTCCTG; ACTGGTCAGGAAGAAATTGATACTGCTTGTGAGATCCTGTATGAAAGAATGAAATCCCTG; GGACCTGATGTTCCAGAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCCCAGTGAGATG; CAGACCCGAATCTTTGACCCAGCTCCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAAT; ATCGCAGAGACATCGCTGACTATTGATGGTATCTACTATGTGGTGGACCCAGGATTCGTG; AAACAGAAAGTTTACAATTCCAAGACAGGGATTGACCAGCTCGTGGTGACGCCTATTTCT; CAGGCTCAGGCAAAGCAACGAGCTGGCAGAGCTGGGAGAACAGGCCCAGGGAAGTGTTAC; AGGTTGTACACAGAACGTGCCTACCGAGATGAAATGCTGACCACCAACGTGCCGGAAATC; CAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGGCCATGGGTATCAATGATCTG; CTGTCCTTTGATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGCCATGGAGCAG; CTGTACACACTGGGGGCCCTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATG; GCAGAGTTCCCTCTGGAGCCAATGCTATGCAAAATGCTCATCATGTCTGTGCATCTGGGC; TGCAGTGAGGAAATGCTGACCATTGTATCCATGCTGTCTGTGCAGAACGTCTTCTATAGG; CCCAAGGATAAACAAGCCCTTGCAGATCAGAAGAAGGCCAAATTCCACCAGACTGAAGGG; GACCACCTCACCCTGCTAGCTGTGTACAACTCCTGGAAGAACAACAAGTTCTCCAACCCA; TGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCCGGGCCCAGGACATTCGCAAG; CAGATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGGCAAGTCCACA; GTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCG; CAGGAGGGTTACCGGACACTGATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCC; CTCTTCAACAGACAGCCAGAATGGGTGGTGTACCATGAGCTGGTGCTCACCACCAAGGAA; TACATGCGTGAAGTTACCACCATCGACCCTCGGTGGCTTGTGGAGTTTGCCCCAGCCTTC; TTCAAGGTCTCAGACCCAACTAAGCTAAGCAAACAGAAGAAGCAACAGCGTCTTGAACCC; TTGTACAACCGCTATGAGGAACCCAATGCCTGGAGAATATCTCGAGCTTTCCGACGGCGC; TGA; ; Clone variation with respect to NM_004941.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004941 unedited; GGGTTTTAGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGCCCGGCC; GGAGTAGCTCTGAGCGCCGGCTGTGAGGAAGGAGGTTCTGGGCAAGCTATAGCCATGGCT; GTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTT; GCCAAACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTG; GGGATCAACGACAAGGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACC; TTTGATACTTTTAAGGCTTCTCTCGTCAAAAATGGTGCAGAATTTACGGATTCTCTTATT; AGTAACTTGCTGCGTCTCATACAAACCATGCGGCCTCCAGCGAAGCCTTCCACTAGCAAA; GATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGAACTCTTCCCAGTCCTT; TGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAAGTTGCT; GTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAGCAA; AGAGATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGA; AACCGAGATCGAGACAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACAC; CGATCCCGCTCTCGATCACGTTCCAGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATAT; CGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAGACCGGAGGNACCGGGACAATATGGAGA; GCGGAATCTGGNATAGATGGCGGATAAGCATGTGGACCGCCCTCCTCCAGAAGAGCCACC; ATTGGTGACATCTATAATGGCAAGNT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004941 unedited; AAGCTTGGACCCGCGGCCGCAATCTAGTGATCAGTTTTTTTTTTTTTTTTTTGGTTCCTT; AAGAAAAAAATATTTCCACGAGGTCAAGCCAAAGTACAGGGGCCTGGAGACGGGGTGGGA; AGTGGGGCAAACACTTTAGATGAGCACAGTAGCTCCTGCTAGCTGGGGCATCAGGGCACT; CACCATGTCCCTGCACAAGATGCTCACCCACTCCACCTTCCCATCCTGGAGCTGCACAAA; TTGTCCCTTTCTTGAAGTTGATGCAATGTGTCTCTGTGTTAGATATGAGCTCCCAACTGG; GCCCAAGGACTTGGGGTGATGGAGGCTGCAGGCTCTTGCCAAAGCCAGGTTTAAATCCCT; GTTTCTATTTTACCAGCAGGGAAGAAATGAGAGTCGAGTTGAGAAAATGCTCAGTTAAGA; CAGTCAGTCACGGGACAGCTGTATCCTCAGGACCAGCCTGTCATCGATAAGTCCAAGCCC; TGGCTACTGCTGCTGGAGAGGCAAAGGAACAATCTTGCCTTTCAGCGCCGTCGGAAAGCT; CGAGATATTCTCCAGGCATTGGGTTCCTCATAGCGGTTGTACAAGGGTTCAAGACGCTGT; TGCTTCTTCTGTTTGCTTAACTTAATTGGGTCTGAGACCTTGAAGAAGGCTGGGGCAAAC; TCCACAAGCCACCGAGGGTCGATGGTGGTAACTTCACGCATGTATTCCTTGGTGGTGAGC; ACCAGCTCATGGTACACCACCCATTCTGGCTGTCTGTTGAAAAGGGCACTGGAAGGATGG; ATATAGACCACCTGCTGGTCGATCAGTGTCCGGTAACCCTCCTGCCGGTCTTTCTTGGCA; GCATTACGGAAGAACCCCCTGCAGATGGCCTTCTGCACTCNGACTGTGGACTTGCCACAG; GAAAAACATNCCACCTTGGGGCTGGCCATTTGGCCAACATTCGCTTGGGAATGTCCCTGG; GCCCGCGCCA;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004941
• Insert Size: 4640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004941.1, NP_004932.1
• RefSeq Size: 4201 bp
• RefSeq ORF: 3663 bp
• Locus ID: 1659
• UniProt ID: Q14562
• Cytogenetics: 17q21.31
• Domains: DEAD, HA2, helicase_C, S1
• Protein Pathways: Spliceosome
• Summary: This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]