LZTFL1 (NM_020347) Human Tagged ORF Clone

SKU
RG207289
LZTFL1 (tGFP-tagged) - Human leucine zipper transcription factor-like 1 (LZTFL1)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $500.00 MSRP $500.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LZTFL1
Synonyms BBS17
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG207289 representing NM_020347
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGTTGGGCCTAAATGAGCACCATCAAAATGAAGTTATTAATTATATGCGTTTTGCTCGTTCAA
AGAGAGGCTTGAGACTCAAAACTGTAGATTCCTGCTTCCAAGACCTCAAGGAGAGCAGGCTGGTGGAGGA
CACCTTCACCATAGATGAAGTCTCTGAAGTCCTCAATGGATTACAAGCTGTGGTTCATAGTGAGGTGGAA
TCTGAGCTCATCAACACTGCCTATACCAATGTGTTACTTCTGCGACAGCTGTTTGCACAAGCTGAGAAGT
GGTATCTTAAGCTACAGACAGACATCTCTGAACTTGAAAACCGAGAATTATTAGAACAAGTTGCAGAATT
TGAAAAAGCAGAGATTACATCTTCAAACAAAAAGCCCATCTTAGATGTCACAAAGCCAAAACTTGCTCCA
CTTAATGAAGGTGGAACAGCAGAACTCCTAAACAAGGAAATTTTAAGACTTCAAGAAGAGAATGAGAAAT
TGAAGTCAAGGTTGAAGACCATTGAAATACAGGCTACAAATGCACTGGATGAAAAGTCAAAACTAGAAAA
AGCACTGCAAGATTTACAGCTTGATCAAGGAAATCAAAAGGATTTTATAAAGGCCCAAGACTTAAGTAAC
TTAGAAAACACTGTCGCTGCCTTAAAGAGTGAGTTTCAGAAGACACTTAATGACAAGACAGAAAACCAGA
AGTCACTGGAGGAGAATCTGGCGACAGCCAAGCACGATCTACTCAGGGTTCAGGAGCAGCTGCACATGGC
TGAAAAGGAATTAGAAAAGAAATTTCAGCAAACAGCAGCTTATCGAAACATGAAAGAGATTCTTACCAAG
AAGAATGACCAAATCAAAGATCTGAGGAAAAGACTGGCACAATATGAACCTGAAGAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG207289 representing NM_020347
Red=Cloning site Green=Tags(s)

MAELGLNEHHQNEVINYMRFARSKRGLRLKTVDSCFQDLKESRLVEDTFTIDEVSEVLNGLQAVVHSEVE
SELINTAYTNVLLLRQLFAQAEKWYLKLQTDISELENRELLEQVAEFEKAEITSSNKKPILDVTKPKLAP
LNEGGTAELLNKEILRLQEENEKLKSRLKTIEIQATNALDEKSKLEKALQDLQLDQGNQKDFIKAQDLSN
LENTVAALKSEFQKTLNDKTENQKSLEENLATAKHDLLRVQEQLHMAEKELEKKFQQTAAYRNMKEILTK
KNDQIKDLRKRLAQYEPED

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_020347
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020347.4
RefSeq Size 3398 bp
RefSeq ORF 900 bp
Locus ID 54585
UniProt ID Q9NQ48
Cytogenetics 3p21.31
Protein Families Transcription Factors
Summary This gene encodes a ubiquitously expressed protein that localizes to the cytoplasm. This protein interacts with Bardet-Biedl Syndrome (BBS) proteins and, through its interaction with BBS protein complexes, regulates protein trafficking to the ciliary membrane. Nonsense mutations in this gene cause a form of Bardet-Biedl Syndrome; a ciliopathy characterized in part by polydactyly, obesity, cognitive impairment, hypogonadism, and kidney failure. This gene may also function as a tumor suppressor; possibly by interacting with E-cadherin and the actin cytoskeleton and thereby regulating the transition of epithelial cells to mesenchymal cells. [provided by RefSeq, Aug 2020]
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SKU Description Size Price
RC207289 LZTFL1 (Myc-DDK-tagged)-Human leucine zipper transcription factor-like 1 (LZTFL1) 10 ug
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RC207289L1 Lenti ORF clone of Human leucine zipper transcription factor-like 1 (LZTFL1), Myc-DDK-tagged 10 ug
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RC207289L2 Lenti ORF clone of Human leucine zipper transcription factor-like 1 (LZTFL1), mGFP tagged 10 ug
$600.00
RC207289L3 Lenti ORF clone of Human leucine zipper transcription factor-like 1 (LZTFL1), Myc-DDK-tagged 10 ug
$600.00
RC207289L4 Lenti ORF clone of Human leucine zipper transcription factor-like 1 (LZTFL1), mGFP tagged 10 ug
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SC113133 LZTFL1 (untagged)-Human leucine zipper transcription factor-like 1 (LZTFL1) 10 ug
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Citations

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