LZTFL1 (NM_020347) Human Untagged Clone

SKU SC113133

LZTFL1 (untagged)-Human leucine zipper transcription factor-like 1 (LZTFL1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LZTFL1
• Synonyms: BBS17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113133 sequence for NM_020347 edited (data generated by NextGen Sequencing); ATGGCAGAGTTGGGCCTAAATGAGCACCATCAAAATGAAGTTATTAATTATATGCGTTTT; GCTCGTTCAAAGAGAGGCTTGAGACTCAAAACTGTAGATTCCTGCTTCCAAGACCTCAAG; GAGAGCAGGCTGGTGGAGGACACCTTCACCATAGATGAAGTCTCTGAAGTCCTCAATGGA; TTACAAGCTGTGGTTCATAGTGAGGTGGAATCTGAGCTCATCAACACTGCCTATACCAAT; GTGTTACTTCTGCGACAGCTGTTTGCACAAGCTGAGAAGTGGTATCTTAAGCTACAGACA; GACATCTCTGAACTTGAAAACCGAGAATTATTAGAACAAGTTGCAGAATTTGAAAAAGCA; GAGATTACATCTTCAAACAAAAAGCCCATCTTAGATGTCACAAAGCCAAAACTTGCTCCA; CTTAATGAAGGTGGAACAGCAGAACTCCTAAACAAGGAAATTTTAAGACTTCAAGAAGAG; AATGAGAAATTGAAGTCAAGGTTGAAGACCATTGAAATACAGGCTACAAATGCACTGGAT; GAAAAGTCAAAACTAGAAAAAGCACTGCAAGATTTACAGCTTGATCAAGGAAATCAAAAG; GATTTTATAAAGGCCCAAGACTTAAGTAACTTAGAAAACACTGTCGCTGCCTTAAAGAGT; GAGTTTCAGAAGACACTTAATGACAAGACAGAAAACCAGAAGTCACTGGAGGAGAATCTG; GCGACAGCCAAGCACGATCTACTCAGGGTTCAGGAGCAGCTGCACATGGCTGAAAAGGAA; TTAGAAAAGAAATTTCAGCAAACAGCAGCTTATCGAAACATGAAAGAGATTCTTACCAAG; AAGAATGACCAAATCAAAGATCTGAGGAAAAGACTGGCACAATATGAACCTGAAGATTAA; ; Clone variation with respect to NM_020347.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020347 unedited; NCCGTTCAGATTTTGTATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGA; TTTTCTGTGGTCCAACTACCCTCGGCGATCCCAGGCTTGGCGGGGCACCGCCTGGCCTCT; CCCGTTCCTTTAGGCTGCCGCCGCTGCCTGCCGCCATGGCAGAGTTGGGCCTAAATGAGC; ACCATCAAAATGAAGTTATTAATTATATGCGTTTTGCTCGTTCAAAGAGAGGCTTGAGAC; TCAAAACTGTAGATTCCTGCTTCCAAGACCTCAAGGAGAGCAGGCTGGTGGAGGACACCT; TCACCATAGATGAAGTCTCTGAAGTCCTCAATGGATTACAAGCTGTGGTTCATAGTGAGG; TGGAATCTGAGCTCATCAACACTGCCTATACCAATGTGTTACTTCTGCGACAGCTGTTTG; CACAAGCTGAGAAGTGGTATCTTAAGCTACAGACAGACATCTCTGAACTTGAAAACCGAG; AATTATTAGAACAAGTTGCAGAATTTGAAAAAGCAGAGATTACATCTTCAAACAAAAAGC; CCATCTTAGATGTCACAAAGCCAAAACTTGCTCCACTTAATGAAGGTGGAACAGCAGAAC; TCCTAAACAAGGAAATTTTAAGACTTCAAGAAGAGAATGAGAAATTGAAGTCAAGGTTGA; AGACCATTGAAATACAGGCTACAAATGCACTGGATGAAAAGTCAAAACTAGAAAAAGCAC; TGCAAGATTTACAGCTTGATCAAGGAAATCAAAAGGATTNTATAAAGGGCCCAAGACTTT; AAGTACTTAGAAAACACTGTCGCTGCCTTAAAGNAGTGAGTTCAGAAGACACTTNTATGA; CAGACAGAAAACCGAAGTCACTGGNAGNAGAATCTGGCGACAGCCAAGCCGATCTACTCA; GGGGTCAGGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020347 unedited; TGGCACTTCCAGGGCCGGNATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCT; GTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTT; TAAAAATAAAGAGACATATTTATTTGTAATAACTCTCTTTTTTTGGTAATAACTATCTTT; AACAATGCTATTATTTATTTTTACCCATGGGGAATGCATAAGTACAAACATTGTTAGCTT; ATTTAACATTTATTAAAAATACAGACTCTTTATACTAATGCAGAAGGATAAGCCTCTTCT; TCCCACTCTAAAAATCTCTTCTTTGCTGTAAAGAATTCTCAGTGCATGTGAGCTAAGACT; CTGGAGCACTCAGTAGAGAGGTGTGTGGGATGACCAGACAGAATCACTAGGTTTTCTCTG; TTTTCAACTAGCTGAAAATAGGAACTCTAGTTCTAAATATTCAAAGTCTAAATATTAGAA; AAATAAGGAGAGGGGATATGACAAAATGCTTTTCAAAATACATGCCTGAGGTGAGACTGC; TTGTATGTTTGCATGTGGTAGCTTCCAGAGGAAATCTTCAGTTTTAATCTTCAGGTTCAT; ATTGTGCCAGTCTTTTCCTCAGATCTTTGATTTGGTCATTCTTCTTGGTAGAATCTCTTT; CATGTTTCGATAAGCTGCTGTTTGCTGAAATTTCTTTTCTAATTCCTTTTCAGCCATGTG; CAGCTGCTCCTGAACCCTGAATAGATCGTGCTTGGCTGTCGCAGATTTCCTCCANGACTT; CTGGTTTCTGTCTTGCATTAAGGGTCTCTGAAACNACTCTTAAGCAGCGACAGTGTTCTA; AGTACTTAGTCTTGGGCTTATAAATC;
• Restriction Sites: NotI-NotI
• ACCN: NM_020347
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020347.2, NP_065080.1
• RefSeq Size: 3398 bp
• RefSeq ORF: 900 bp
• Locus ID: 54585
• UniProt ID: Q9NQ48
• Cytogenetics: 3p21.31
• Protein Families: Transcription Factors
• Summary: This gene encodes a ubiquitously expressed protein that localizes to the cytoplasm. This protein interacts with Bardet-Biedl Syndrome (BBS) proteins and, through its interaction with BBS protein complexes, regulates protein trafficking to the ciliary membrane. Nonsense mutations in this gene cause a form of Bardet-Biedl Syndrome; a ciliopathy characterized in part by polydactyly, obesity, cognitive impairment, hypogonadism, and kidney failure. This gene may also function as a tumor suppressor; possibly by interacting with E-cadherin and the actin cytoskeleton and thereby regulating the transition of epithelial cells to mesenchymal cells. [provided by RefSeq, Aug 2020]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.