GTP binding protein era homolog (ERAL1) (NM_005702) Human Tagged ORF Clone

SKU
RG205710
ERAL1 (tGFP-tagged) - Human Era G-protein-like 1 (E. coli) (ERAL1)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $657.00 MSRP $657.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GTP binding protein era homolog
Synonyms CEGA; ERA; ERA-W; ERAL1A; ERAL1B; H-ERA; HERA-A; HERA-B; PRLTS6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205710 representing NM_005702
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCCCCAGCTGGCGCGGGGCTAGGCTTGTTCAATCGGTGTTAAGAGTCTGGCAGGTGGGCCCTC
ATGTCGCGAGGGAGCGGGTGATCCCTTTTTCCTCACTCTTAGGCTTCCAACGGAGGTGCGTGTCCTGCGT
CGCGGGGTCCGCTTTCTCTGGTCCCCGCTTGGCCTCGGCTTCTCGCAGTAATGGCCAGGGCTCTGCCCTG
GACCACTTCCTCGGATTCTCTCAGCCCGACAGTTCGGTGACTCCTTGCGTCCCCGCGGTGTCCATGAACA
GAGATGAGCAGGATGTCCTCTTGGTCCATCACCCTGATATGCCTGAGAATTCCCGGGTCCTACGAGTGGT
CCTCCTGGGAGCCCCGAATGCAGGGAAGTCAACACTCTCCAACCAGCTACTGGGCCGAAAGGTGTTCCCT
GTTTCCAGGAAGGTGCATACTACTCGCTGCCAAGCTCTGGGGGTCATCACAGAGAAGGAGACCCAGGTGA
TTCTACTTGACACACCTGGCATTATCAGTCCTGGTAAACAGAAGAGGCATCACCTGGAGCTCTCTTTGTT
GGAAGATCCATGGAAGAGCATGGAATCTGCTGATCTTGTTGTGGTTCTTGTGGATGTCTCAGACAAGTGG
ACACGGAACCAGCTCAGCCCCCAGTTGCTCAGGTGCTTGACCAAGTACTCCCAGATCCCTAGTGTCCTGG
TCATGAACAAGGTAGATTGTTTGAAGCAGAAGTCAGTTCTCCTGGAGCTCACGGCAGCCCTCACTGAAGG
TGTGGTCAATGGCAAAAAGCTCAAGATGAGGCAGGCCTTCCACTCACACCCTGGCACCCATTGCCCCAGC
CCAGCAGTTAAGGACCCAAACACACAATCTGTGGGAAATCCTCAGAGGATTGGCTGGCCCCACTTCAAGG
AGATCTTCATGTTGTCAGCCCTAAGCCAGGAGGACGTGAAAACACTAAAGCAATACCTTCTGACACAGGC
CCAGCCAGGGCCCTGGGAGTACCACAGTGCAGTCCTCACTAGCCAGACACCAGAAGAGATCTGTGCCAAC
ATTATCCGAGAGAAGCTCCTAGAACACCTGCCCCAGGAGGTGCCTTACAATGTACAGCAGAAGACAGCAG
TGTGGGAGGAAGGACCAGGTGGGGAGCTGGTTATCCAACAGAAGCTTCTGGTGCCCAAAGAATCTTATGT
GAAACTCCTGATTGGTCCGAAGGGCCACGTGATCTCCCAGATAGCACAGGAGGCAGGCCATGACCTCATG
GACATCTTCCTCTGCGATGTTGACATCCGCCTCTCTGTGAAGCTCCTCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205710 representing NM_005702
Red=Cloning site Green=Tags(s)

MAAPSWRGARLVQSVLRVWQVGPHVARERVIPFSSLLGFQRRCVSCVAGSAFSGPRLASASRSNGQGSAL
DHFLGFSQPDSSVTPCVPAVSMNRDEQDVLLVHHPDMPENSRVLRVVLLGAPNAGKSTLSNQLLGRKVFP
VSRKVHTTRCQALGVITEKETQVILLDTPGIISPGKQKRHHLELSLLEDPWKSMESADLVVVLVDVSDKW
TRNQLSPQLLRCLTKYSQIPSVLVMNKVDCLKQKSVLLELTAALTEGVVNGKKLKMRQAFHSHPGTHCPS
PAVKDPNTQSVGNPQRIGWPHFKEIFMLSALSQEDVKTLKQYLLTQAQPGPWEYHSAVLTSQTPEEICAN
IIREKLLEHLPQEVPYNVQQKTAVWEEGPGGELVIQQKLLVPKESYVKLLIGPKGHVISQIAQEAGHDLM
DIFLCDVDIRLSVKLLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_005702
ORF Size 1311 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005702.4
RefSeq Size 1856 bp
RefSeq ORF 1314 bp
Locus ID 26284
UniProt ID O75616
Cytogenetics 17q11.2
Summary The protein encoded by this gene is a GTPase that localizes to the mitochondrion. The encoded protein binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of this gene has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]
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Citations

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