GTP binding protein era homolog (ERAL1) (NM_005702) Human Untagged Clone

SKU SC101031

ERAL1 (untagged)-Human Era G-protein-like 1 (E. coli) (ERAL1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GTP binding protein era homolog
• Synonyms: CEGA; ERA; ERA-W; ERAL1A; ERAL1B; H-ERA; HERA-A; HERA-B; PRLTS6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101031 sequence for NM_005702 edited (data generated by NextGen Sequencing); ATGGCTGCCCCCAGCTGGCGCGGGGCTAGGCTTGTTCAATCGGTGTTAAGAGTCTGGCAG; GTGGGCCCTCATGTCGCGAGGGAGCGGGTGATCCCTTTTTCCTCACTCTTAGGCTTCCAA; CGGAGGTGCGTGTCCTGCGTCGCGGGGTCCGCTTTCTCTGGTCCCCGCTTGGCCTCGGCT; TCTCGCAGTAATGGCCAGGGCTCTGCCCTGGACCACTTCCTCGGATTCTCTCAGCCCGAC; AGTTCGGTGACTCCTTGCGTCCCCGCGGTGTCCATGAACAGAGATGAGCAGGATGTCCTC; TTGGTCCATCACCCTGATATGCCTGAGAATTCCCGGGTCCTACGAGTGGTCCTCCTGGGA; GCCCCGAATGCAGGGAAGTCAACACTCTCCAACCAGCTACTGGGCCGAAAGGTGTTCCCT; GTTTCCAGGAAGGTGCATACTACTCGCTGCCAAGCTCTGGGGGTCATCACAGAGAAGGAG; ACCCAGGTGATTCTACTTGACACACCTGGCATTATCAGTCCTGGTAAACAGAAGAGGCAT; CACCTGGAGCTCTCTTTGTTGGAAGATCCATGGAAGAGCATGGAATCTGCTGATCTTGTT; GTGGTTCTTGTGGATGTCTCAGACAAGTGGACACGGAACCAGCTCAGCCCCCAGTTGCTC; AGGTGCTTGACCAAGTACTCCCAGATCCCTAGTGTCCTGGTCATGAACAAGGTAGATTGT; TTGAAGCAGAAGTCAGTTCTCCTGGAGCTCACGGCAGCCCTCACTGAAGGTGTGGTCAAT; GGCAAAAAGCTCAAGATGAGGCAGGCCTTCCACTCACACCCTGGCACCCATTGCCCCAGC; CCAGCAGTTAAGGACCCAAACACACAATCTGTGGGAAATCCTCAGAGGATTGGCTGGCCC; CACTTCAAGGAGATCTTCATGTTGTCAGCCCTAAGCCAGGAGGATGTGAAAACACTAAAG; CAATACCTTCTGACACAGGCCCAGCCAGGGCCCTGGGAGTACCACAGTGCAGTCCTCACT; AGCCAGACACCAGAAGAGATCTGTGCCAACATTATCCGAGAGAAGCTCCTAGAACACCTG; CCCCAGGAGGTGCCTTACAATGTACAGCAGAAGACAGCAGTGTGGGAGGAAGGACCAGGT; GGGGAGCTGGTTATCCAACAGAAGCTTCTGGTGCCCAAAGAATCTTATGTGAAACTCCTG; ATTGGTCCGAAGGGCCACGTGATCTCCCAGATAGCACAGGAGGCAGGCCATGACCTCATG; GACATCTTCCTCTGCGATGTTGACATCCGCCTCTCTGTGAAGCTCCTCAAGTGA; ; Clone variation with respect to NM_005702.2; 945 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005702 unedited; NCGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGCTGTA; ATGGCTGCCCCCAGCTGGCGCGGGGCTAGGCTTGTTCAATCGGTGTTAAGAGTCTGGCAG; GTGGGCCCTCATGTCGCGAGGGAGCGGGTGATCCCTTTTTCCTCACTCTTAGGCTTCCAA; CGGAGGTGCGTGTCCTGCGTCGCGGGGTCCGCTTTCTCTGGTCCCCGCTTGGCCTCGGCT; TCTCGCAGTAATGGCCAGGGCTCTGCCCTGGACCACTTCCTCGGATTCTCTCAGCCCGAC; AGTTCGGTGACTCCTTGCGTCCCCGCGGTGTCCATGAACAGAGATGAGCAGGATGTCCTC; TTGGTCCATCACCCTGATATGCCTGAGAATTCCCGGGTCCTACGAGTGGTCCTCCTGGGA; GCCCCGAATGCAGGGAAGTCAACACTCTCCAACCAGCTACTGGGCCGAAAGGTGTTCCCT; GTTTCCAGGAAGGTGCATACTACTCGCTGCCAAGCTCTGGGGGTCATCACAGAGAAGGAG; ACCCAGGTGATTCTACTTGACACACCTGGCATTATCAGTCCTGGTAAACAGAAGAGGCAT; CACCTGGAGCTCTCTTTGTTGGAAGATCCATGGAAGAGCATGGAATCTGCTGATCTTGTT; GTGGTTCTTGTGGATGTCTCAGAACAGTGGACACGGAACCAGCTCAGCCCCCAGTTGCTC; AGGTGCTTGACCAAGTACTCCCAGATCCCTAGTGTCCTGGTCATGAACAAGGTAGATTGT; TTTGAACAGAAGTCAGTTCTCCTGGAGCTCACGGCAGCCCTCACTGAAAGTGTGGTCAAT; GGCAAAAGCTCAAGATGAAGCAGGCCTTTCACTCACACCCTGGCACCCATGGCCCAGCCC; ACCGTTTAAGACCCCAACCCCAATCTGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005702
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005702.2, NP_005693.1
• RefSeq Size: 1856 bp
• RefSeq ORF: 1314 bp
• Locus ID: 26284
• UniProt ID: O75616
• Cytogenetics: 17q11.2
• Summary: The protein encoded by this gene is a GTPase that localizes to the mitochondrion. The encoded protein binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of this gene has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).