ZBTB20 (NM_001164345) Human Tagged ORF Clone

SKU RC228990

ZBTB20 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 5

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ZBTB20
• Synonyms: DPZF; HOF; ODA-8S; PRIMS; ZNF288
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228990 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCGAGCGCATTCACAGCATCAACCTTCACAACTTCAGCAATTCCGTGCTCGAGACCCTCAACGAGC; AGCGCAACCGTGGCCACTTCTGTGACGTAACGGTGCGCATCCACGGGAGCATGCTGCGCGCACACCGCTG; CGTGCTGGCAGCCGGCAGCCCCTTCTTCCAGGACAAACTGCTGCTTGGCTACAGCGACATCGAGATCCCG; TCGGTGGTGTCAGTGCAGTCAGTGCAAAAGCTCATTGACTTCATGTACAGCGGCGTGCTACGGGTCTCGC; AGTCGGAAGCTCTGCAGATCCTCACGGCCGCCAGCATCCTGCAGATCAAAACAGTCATCGACGAGTGCAC; GCGCATCGTGTCACAGAACGTGGGCGATGTGTTCCCGGGGATCCAGGACTCGGGCCAGGACACGCCGCGG; GGCACTCCCGAGTCAGGCACGTCAGGCCAGAGCAGCGACACGGAGTCGGGCTACCTGCAGAGCCACCCAC; AGCACAGCGTGGACAGGATCTACTCGGCACTCTACGCGTGCTCCATGCAGAATGGCAGCGGCGAGCGCTC; TTTTTACAGCGGCGCAGTGGTCAGCCACCACGAGACTGCGCTCGGCCTGCCCCGCGACCACCACATGGAA; GACCCCAGCTGGATCACACGCATCCATGAGCGCTCGCAGCAGATGGAGCGCTACCTGTCCACCACCCCCG; AGACCACGCACTGCCGCAAGCAGCCCCGGCCTGTGCGCATCCAGACCCTAGTGGGCAACATCCACATCAA; GCAGGAGATGGAGGACGATTACGACTACTACGGGCAGCAAAGGGTGCAGATCCTGGAACGCAACGAATCC; GAGGAGTGCACGGAAGACACAGACCAGGCCGAGGGCACCGAGAGTGAGCCCAAAGGTGAAAGCTTCGACT; CGGGCGTCAGCTCCTCCATAGGCACCGAGCCTGACTCGGTGGAGCAGCAGTTTGGGCCTGGGGCGGCGCG; GGACAGCCAGGCTGAACCCACCCAACCCGAGCAGGCTGCAGAAGCCCCCGCTGAGGGTGGTCCGCAGACA; AACCAGCTAGAAACAGGTGCTTCCTCTCCGGAGAGAAGCAATGAAGTGGAGATGGACAGCACTGTTATCA; CTGTCAGCAACAGCTCCGACAAGAGCGTCCTACAACAGCCTTCGGTCAACACGTCCATCGGGCAGCCATT; GCCAAGTACCCAGCTCTACTTACGCCAGACAGAAACCCTCACCAGCAACCTGAGGATGCCTCTGACCTTG; ACCAGCAACACGCAGGTCATTGGCACAGCTGGCAACACCTACCTGCCAGCCCTCTTCACTACCCAGCCCG; CGGGCAGTGGCCCCAAGCCTTTCCTCTTCAGCCTGCCACAGCCCCTGGCAGGCCAGCAGACCCAGTTTGT; GACAGTGTTCCAGCCCGGTCTGTCGACCTTTACTGCACAGCTGCCAGCGCCACAGCCCCTGGCCTCATCC; GCAGGCCACAGCACAGCCAGTGGGCAAGGCGAAAAAAAGCCTTATGAGTGCACTCTCTGCAACAAGACTT; TCACCGCCAAACAGAACTACGTCAAGCACATGTTCGTACACACAGGTGAGAAGCCCCACCAATGCAGCAT; CTGTTGGCGCTCCTTCTCCTTAAAGGATTACCTTATCAAGCACATGGTGACACACACAGGAGTGAGGGCA; TACCAGTGTAGTATCTGCAACAAGCGCTTCACCCAGAAGAGCTCCCTCAACGTGCACATGCGCCTCCACC; GGGGAGAGAAGTCCTACGAGTGCTACATCTGCAAAAAGAAGTTCTCTCACAAGACCCTCCTGGAGCGACA; CGTGGCCCTGCACAGTGCCAGCAATGGGACCCCCCCTGCAGGCACACCCCCAGGTGCCCGCGCTGGCCCC; CCAGGCGTGGTGGCCTGCACGGAGGGGACCACTTACGTCTGCTCCGTCTGCCCAGCAAAGTTTGACCAAA; TCGAGCAGTTCAACGACCACATGAGGATGCATGTGTCTGACGGA; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228990 protein sequence; Red=Cloning site Green=Tags(s); ; MTERIHSINLHNFSNSVLETLNEQRNRGHFCDVTVRIHGSMLRAHRCVLAAGSPFFQDKLLLGYSDIEIP; SVVSVQSVQKLIDFMYSGVLRVSQSEALQILTAASILQIKTVIDECTRIVSQNVGDVFPGIQDSGQDTPR; GTPESGTSGQSSDTESGYLQSHPQHSVDRIYSALYACSMQNGSGERSFYSGAVVSHHETALGLPRDHHME; DPSWITRIHERSQQMERYLSTTPETTHCRKQPRPVRIQTLVGNIHIKQEMEDDYDYYGQQRVQILERNES; EECTEDTDQAEGTESEPKGESFDSGVSSSIGTEPDSVEQQFGPGAARDSQAEPTQPEQAAEAPAEGGPQT; NQLETGASSPERSNEVEMDSTVITVSNSSDKSVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMPLTL; TSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLAGQQTQFVTVFQPGLSTFTAQLPAPQPLASS; AGHSTASGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTGVRA; YQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGARAGP; PGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG; ; SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164345
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001164345.3
• RefSeq Size: 27291 bp
• RefSeq ORF: 2007 bp
• Locus ID: 26137
• UniProt ID: Q9HC78
• Cytogenetics: 3q13.31
• Protein Families: Transcription Factors
• MW: 73.5 kDa
• Summary: This gene, which was initially designated as dendritic cell-derived BTB/POZ zinc finger (DPZF), belongs to a family of transcription factors with an N-terminal BTB/POZ domain and a C-terminal DNA-bindng zinc finger domain. The BTB/POZ domain is a hydrophobic region of approximately 120 aa which mediates association with other BTB/POZ domain-containing proteins. This gene acts as a transcriptional repressor and plays a role in many processes including neurogenesis, glucose homeostasis, and postnatal growth. Mutations in this gene have been associated with Primrose syndrome as well as the 3q13.31 microdeletion syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]