ZBTB20 (NM_001164345) Human Untagged Clone

SKU SC327625

ZBTB20 (untagged)-Human zinc finger and BTB domain containing 20 (ZBTB20) transcript variant 5

Product Data

• Type: Human Untagged Clone
• Target Symbol: ZBTB20
• Synonyms: DPZF; HOF; ODA-8S; PRIMS; ZNF288
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164345, the custom clone sequence may differ by one or more nucleotides; ATGACCGAGCGCATTCACAGCATCAACCTTCACAACTTCAGCAATTCCGTGCTCGAGACC; CTCAACGAGCAGCGCAACCGTGGCCACTTCTGTGACGTAACGGTGCGCATCCACGGGAGC; ATGCTGCGCGCACACCGCTGCGTGCTGGCAGCCGGCAGCCCCTTCTTCCAGGACAAACTG; CTGCTTGGCTACAGCGACATCGAGATCCCGTCGGTGGTGTCAGTGCAGTCAGTGCAAAAG; CTCATTGACTTCATGTACAGCGGCGTGCTACGGGTCTCGCAGTCGGAAGCTCTGCAGATC; CTCACGGCCGCCAGCATCCTGCAGATCAAAACAGTCATCGACGAGTGCACGCGCATCGTG; TCACAGAACGTGGGCGATGTGTTCCCGGGGATCCAGGACTCGGGCCAGGACACGCCGCGG; GGCACTCCCGAGTCAGGCACGTCAGGCCAGAGCAGCGACACGGAGTCGGGCTACCTGCAG; AGCCACCCACAGCACAGCGTGGACAGGATCTACTCGGCACTCTACGCGTGCTCCATGCAG; AATGGCAGCGGCGAGCGCTCTTTTTACAGCGGCGCAGTGGTCAGCCACCACGAGACTGCG; CTCGGCCTGCCCCGCGACCACCACATGGAAGACCCCAGCTGGATCACACGCATCCATGAG; CGCTCGCAGCAGATGGAGCGCTACCTGTCCACCACCCCCGAGACCACGCACTGCCGCAAG; CAGCCCCGGCCTGTGCGCATCCAGACCCTAGTGGGCAACATCCACATCAAGCAGGAGATG; GAGGACGATTACGACTACTACGGGCAGCAAAGGGTGCAGATCCTGGAACGCAACGAATCC; GAGGAGTGCACGGAAGACACAGACCAGGCCGAGGGCACCGAGAGTGAGCCCAAAGGTGAA; AGCTTCGACTCGGGCGTCAGCTCCTCCATAGGCACCGAGCCTGACTCGGTGGAGCAGCAG; TTTGGGCCTGGGGCGGCGCGGGACAGCCAGGCTGAACCCACCCAACCCGAGCAGGCTGCA; GAAGCCCCCGCTGAGGGTGGTCCGCAGACAAACCAGCTAGAAACAGGTGCTTCCTCTCCG; GAGAGAAGCAATGAAGTGGAGATGGACAGCACTGTTATCACTGTCAGCAACAGCTCCGAC; AAGAGCGTCCTACAACAGCCTTCGGTCAACACGTCCATCGGGCAGCCATTGCCAAGTACC; CAGCTCTACTTACGCCAGACAGAAACCCTCACCAGCAACCTGAGGATGCCTCTGACCTTG; ACCAGCAACACGCAGGTCATTGGCACAGCTGGCAACACCTACCTGCCAGCCCTCTTCACT; ACCCAGCCCGCGGGCAGTGGCCCCAAGCCTTTCCTCTTCAGCCTGCCACAGCCCCTGGCA; GGCCAGCAGACCCAGTTTGTGACAGTGTCCCAGCCCGGTCTGTCGACCTTTACTGCACAG; CTGCCAGCGCCACAGCCCCTGGCCTCATCCGCAGGCCACAGCACAGCCAGTGGGCAAGGC; GAAAAAAAGCCTTATGAGTGCACTCTCTGCAACAAGACTTTCACCGCCAAACAGAACTAC; GTCAAGCACATGTTCGTACACACAGGTGAGAAGCCCCACCAATGCAGCATCTGTTGGCGC; TCCTTCTCCTTAAAGGATTACCTTATCAAGCACATGGTGACACACACAGGAGTGAGGGCA; TACCAGTGTAGTATCTGCAACAAGCGCTTCACCCAGAAGAGCTCCCTCAACGTGCACATG; CGCCTCCACCGGGGAGAGAAGTCCTACGAGTGCTACATCTGCAAAAAGAAGTTCTCTCAC; AAGACCCTCCTGGAGCGACACGTGGCCCTGCACAGTGCCAGCAATGGGACCCCCCCTGCA; GGCACACCCCCAGGTGCCCGCGCTGGCCCCCCAGGCGTGGTGGCCTGCACGGAGGGGACC; ACTTACGTCTGCTCCGTCTGCCCAGCAAAGTTTGACCAAATCGAGCAGTTCAACGACCAC; ATGAGGATGCATGTGTCTGACGGA;
• Restriction Sites: Please inquire
• ACCN: NM_001164345
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001164345.1, NP_001157817.1
• RefSeq Size: 3697 bp
• RefSeq ORF: 2007 bp
• Locus ID: 26137
• UniProt ID: Q9HC78
• Cytogenetics: 3q13.31
• Protein Families: Transcription Factors
• Summary: This gene, which was initially designated as dendritic cell-derived BTB/POZ zinc finger (DPZF), belongs to a family of transcription factors with an N-terminal BTB/POZ domain and a C-terminal DNA-bindng zinc finger domain. The BTB/POZ domain is a hydrophobic region of approximately 120 aa which mediates association with other BTB/POZ domain-containing proteins. This gene acts as a transcriptional repressor and plays a role in many processes including neurogenesis, glucose homeostasis, and postnatal growth. Mutations in this gene have been associated with Primrose syndrome as well as the 3q13.31 microdeletion syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]; Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.