PWWP2A (NM_001130864) Human Tagged ORF Clone

SKU
RC226141
PWWP2A (Myc-DDK-tagged)-Human PWWP domain containing 2A (PWWP2A), transcript variant 2
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$589.00 MSRP $691.00 MSRP $691.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PWWP2A
Synonyms MST101
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226141 representing NM_001130864
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGTGGCTGCAGAGGCGGCAGCGACTGCAGCGTCCCCCGGGGAGGGGGGCGCCGGCGAGGCCG
AGCCGGAGATGGAGCCCATCCCCGGCAGTGAGGCCGGCACTGACCCCCTCCCGGTCACGGCCACTGAAGC
GTCTGTGCCGGATGGCGAGACTGACGGGCAGCAATCCGCTCCTCAGGCCGACGAGCCGCCGCTCCCGCCG
CCACCGCCGCCGCCGGGGGAGCTCGCCCGCAGCCCAGAGGCGGTGGGGCCGGAGCTGGAGGCTGAGGAGA
AACTGTCCGTTCGGGTGGCGGAGTCGGCGGCAGCCGCGCCTCAGGGAGGGCCGGAACTTCCACCTTCTCC
TGCATCGCCGCCGGAGCAGCCCCCGGCTCCCGAGGAGCGCGAGGAGCCGCCGCTGCCTCAGCCCGTAGCC
CCGGCGCTCGTGCCGCCGGCGGGCGGGGACTCCACGGTGTCGCAACTGATCCCGGGCTCGGAGGTGCGGG
TCACGCTGGACCACATCATTGAGGACGCGCTTGTCGTGTCGTTCCGCTTCGGGGAGAAGCTCTTCTCCGG
GGTCCTCATGGATCTGTCCAAAAGGTTTGGGCCCCATGGTATCCCTGTGACAGTATTTCCCAAAAGGGAA
TATAAGGATAAACCAGAAGCCATGCCGCTCCAAAGTAATACATTCCAAGAAGGGACAGAAGTCAAGTGTG
AAGCAAATGGTGCTGTTCCCGATGACCCTTCTCCTGTCCCGCATCCCGAGCTGAGCTTGGCTGAAAGCCT
GTGGACTTCCAAACCACCACCTCTCTTCCATGAAGGAGCACCTTATCCTCCCCCTTTGTTTATCAGGGAC
ACATATAACCAATCAATACCTCAGCCACCTCCTCGGAAAATTAAGCGACCCAAACGAAAAATGTACAGGG
AAGAACCCACTTCAATAATGAATGCTATTAAACTACGACCCAGGCAAGTTCTGTGTGATAAATGTAAAAA
CAGTGTTGTTGCTGAAAAAAAGGAAATTAGAAAAGGTAGTAGTGCAACTGACTCTTCTAAATATGAAGAT
AAAAAACGGAGAAATGAAAGTGTAACTACTGTGAACAAAAAACTGAAAACTGACCATAAAGTGGATGGGA
AAAACCAAAATGAAAGCCAGAAAAGAAATGCTGTGGTTAAGGTTTCAAATATTGCTCACAGCAGAGGCAG
AGTAGTAAAAGTTTCTGCTCAGGCAAATACATCAAAAGCTCAGTTAAGTACTAAAAAAGTTCTCCAGAGT
AAGAACATGGATCATGCGAAAGCTCGGGAAGTGTTAAAAATTGCCAAAGAAAAGGCACAAAAGAAGCAAA
ATGAAACCTCTACTTCCAAAAATGCACATTCAAAAGTCCATTTCACACGTCGATATCAGAATCCTAGCTC
AGGTTCCCTTCCACCCCGGGTTCGTTTAAAACCACAGAGGTACAGGAATGAAGAAAATGACTCTTCTCTG
AAGACAGGACTTGAGAAAATGCGGAGTGGCAAGATGGCACCCAAGCCCCAGTCTCGCTGCACCTCTACCC
GCTCAGCAGGTGAGGCCCCTTCAGAAAATCAGAGTCCCTCAAAAGGCCCTGAAGAGGCCAGCAGTGAGGT
TCAGGACACAAATGAAGTGCATGTGCCTGGTGATCAGGATGAACCACAGACATTGGGCAAAAAGGGCAGC
AAAAACAATATCTCTGTTTATATGACCCTAAATCAAAAGAAATCTGACTCTTCCAGTGCTTCAGTGTGTA
GCATTGATAGCACAGATGATTTGAAATCTTCCAACTCTGAGTGTAGTTCTTCTGAAAGCTTTGATTTTCC
TCCAGGCAGTATGCATGCACCTTCCACCTCCTCCACTTCCTCCTCTTCAAAGGAAGAGAAAAAGCTCAGT
AATTCCTTGAAAATGAAAGTCTTTTCCAAAAACGTCTCTAAATGCGTCACACCAGATGGCAGGACCATAT
GTGTAGGGGACATTGTTTGGGCCAAGATATATGGCTTCCCTTGGTGGCCAGCCCGTATTCTTACTATAAC
TGTGAGCCGGAAAGATAACGGCCTTTTAGTCCGACAGGAGGCCCGTATTTCATGGTTTGGGTCTCCAACA
ACATCTTTCCTTGCTCTTTCACAACTCTCCCCCTTTTTAGAAAACTTCCAGTCACGCTTTAATAAGAAGA
GAAAGGGCCTGTATCGCAAGGCTATCACAGAGGCAGCTAAGGCTGCCAAGCAGCTGACCCCCGAAGTGCG
GGCTTTGTTGACACAGTTTGAAACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC226141 representing NM_001130864
Red=Cloning site Green=Tags(s)

MAAVAAEAAATAASPGEGGAGEAEPEMEPIPGSEAGTDPLPVTATEASVPDGETDGQQSAPQADEPPLPP
PPPPPGELARSPEAVGPELEAEEKLSVRVAESAAAAPQGGPELPPSPASPPEQPPAPEEREEPPLPQPVA
PALVPPAGGDSTVSQLIPGSEVRVTLDHIIEDALVVSFRFGEKLFSGVLMDLSKRFGPHGIPVTVFPKRE
YKDKPEAMPLQSNTFQEGTEVKCEANGAVPDDPSPVPHPELSLAESLWTSKPPPLFHEGAPYPPPLFIRD
TYNQSIPQPPPRKIKRPKRKMYREEPTSIMNAIKLRPRQVLCDKCKNSVVAEKKEIRKGSSATDSSKYED
KKRRNESVTTVNKKLKTDHKVDGKNQNESQKRNAVVKVSNIAHSRGRVVKVSAQANTSKAQLSTKKVLQS
KNMDHAKAREVLKIAKEKAQKKQNETSTSKNAHSKVHFTRRYQNPSSGSLPPRVRLKPQRYRNEENDSSL
KTGLEKMRSGKMAPKPQSRCTSTRSAGEAPSENQSPSKGPEEASSEVQDTNEVHVPGDQDEPQTLGKKGS
KNNISVYMTLNQKKSDSSSASVCSIDSTDDLKSSNSECSSSESFDFPPGSMHAPSTSSTSSSSKEEKKLS
NSLKMKVFSKNVSKCVTPDGRTICVGDIVWAKIYGFPWWPARILTITVSRKDNGLLVRQEARISWFGSPT
TSFLALSQLSPFLENFQSRFNKKRKGLYRKAITEAAKAAKQLTPEVRALLTQFET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001130864
ORF Size 2265 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001130864.2
RefSeq ORF 2268 bp
Locus ID 114825
UniProt ID Q96N64
Cytogenetics 5q33.3
MW 81.8 kDa
Summary H2A.Z-specific chromatin binding protein which may play an important role in the neural crest stem cell migration and differentiation during early development. Also required for proper mitosis progression.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC226141L1 Lenti ORF clone of Human PWWP domain containing 2A (PWWP2A), transcript variant 2, Myc-DDK-tagged 10 ug
$991.00
RC226141L2 Lenti ORF clone of Human PWWP domain containing 2A (PWWP2A), transcript variant 2, mGFP tagged 10 ug
$991.00
RC226141L3 Lenti ORF clone of Human PWWP domain containing 2A (PWWP2A), transcript variant 2, Myc-DDK-tagged 10 ug
$991.00
RC226141L4 Lenti ORF clone of Human PWWP domain containing 2A (PWWP2A), transcript variant 2, mGFP tagged 10 ug
$991.00
RG226141 PWWP2A (tGFP-tagged) - Human PWWP domain containing 2A (PWWP2A), transcript variant 2 10 ug
$891.00
SC326222 PWWP2A (untagged)-Human PWWP domain containing 2A (PWWP2A), transcript variant 2 10 ug
$761.00

Citations

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