Neuroserpin (SERPINI1) (NM_001122752) Human Tagged ORF Clone

SKU RC225640

SERPINI1 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Neuroserpin
• Synonyms: HNS-S1; HNS-S2; neuroserpin; PI12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225640 representing NM_001122752; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTTTCCTTGGACTCTTCTCTTTGCTGGTTCTGCAAAGTATGGCTACAGGGGCCACTTTCCCTGAGG; AAGCCATTGCTGACTTGTCAGTGAATATGTATAATCGTCTTAGAGCCACTGGTGAAGATGAAAATATTCT; CTTCTCTCCATTGAGTATTGCTCTTGCAATGGGAATGATGGAACTTGGGGCCCAAGGATCTACCCAGGAA; GAAATCCGCCACTCAATGGGATATGACAGCCTAAAAAATGGTGAAGAATTTTCTTTCTTGAAGGAGTTTT; CAAACATGGTAACTGCTAAAGAGAGCCAATATGTGATGAAAATTGCCAATTCCTTGTTTGTGCAAAATGG; ATTTCATGTCAATGAGGAGTTTTTGCAAATGATGAAAAAATATTTTAATGCAGCAGTAAATCATGTGGAC; TTCAGTCAAAATGTAGCCGTGGCCAACTACATCAATAAGTGGGTGGAGAATAACACAAACAATCTGGTGA; AAGATTTGGTATCCCCAAGGGATTTTGATGCTGCCACTTATCTGGCCCTCATTAATGCTGTCTATTTCAA; GGGGAACTGGAAGTCGCAGTTTAGGCCTGAAAATACTAGAACCTTTTCTTTCACTAAAGATGATGAAAGT; GAAGTCCAAATTCCAATGATGTATCAGCAAGGAGAATTTTATTATGGGGAATTTAGTGATGGCTCCAATG; AAGCTGGTGGTATCTACCAAGTCCTAGAAATACCATATGAAGGAGATGAAATAAGCATGATGCTGGTGCT; GTCCAGACAGGAAGTTCCTCTTGCTACTCTGGAGCCATTAGTCAAAGCACAGCTGGTTGAAGAATGGGCA; AACTCTGTGAAGAAGCAAAAAGTAGAAGTATACCTGCCCAGGTTCACAGTGGAACAGGAAATTGATTTAA; AAGATGTTTTGAAGGCTCTTGGAATAACTGAAATTTTCATCAAAGATGCAAATTTGACAGGCCTCTATGA; TAATAAGGAGATTTTTCTTTCCAAAGCAATTCACAAGTCCTTCCTAGAGGTTAATGAAGAAGGCTCAGAA; GCTGCTGCTGTCTCAGGAATGATTGCAATTAGTAGGATGGCTGTGCTGTATCCTCAAGTTATTGTCGACC; ATCCATTTTTCTTTCTTATCAGAAACAGGAGAACTGGTACAATTCTATTCATGGGACGAGTCATGCATCC; TGAAACAATGAACACAAGTGGACATGATTTCGAAGAACTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225640 representing NM_001122752; Red=Cloning site Green=Tags(s); ; MAFLGLFSLLVLQSMATGATFPEEAIADLSVNMYNRLRATGEDENILFSPLSIALAMGMMELGAQGSTQE; EIRHSMGYDSLKNGEEFSFLKEFSNMVTAKESQYVMKIANSLFVQNGFHVNEEFLQMMKKYFNAAVNHVD; FSQNVAVANYINKWVENNTNNLVKDLVSPRDFDAATYLALINAVYFKGNWKSQFRPENTRTFSFTKDDES; EVQIPMMYQQGEFYYGEFSDGSNEAGGIYQVLEIPYEGDEISMMLVLSRQEVPLATLEPLVKAQLVEEWA; NSVKKQKVEVYLPRFTVEQEIDLKDVLKALGITEIFIKDANLTGLYDNKEIFLSKAIHKSFLEVNEEGSE; AAAVSGMIAISRMAVLYPQVIVDHPFFFLIRNRRTGTILFMGRVMHPETMNTSGHDFEEL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122752
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001122752.1, NP_001116224.1
• RefSeq ORF: 1233 bp
• Locus ID: 5274
• UniProt ID: Q99574
• Cytogenetics: 3q26.1
• Protein Families: Druggable Genome, Secreted Protein
• MW: 46.5 kDa
• Summary: This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]