Synapsin I (SYN1) (NM_006950) Human Tagged ORF Clone

SKU
RC221273
SYN1 (Myc-DDK-tagged)-Human synapsin I (SYN1), transcript variant Ia
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Synapsin I
Synonyms EPILX; MRX50; SYN1a; SYN1b; SYNI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221273 representing NM_006950
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTACCTGCGGCGCCGCCTGTCGGACAGCAACTTTATGGCCAATCTGCCAAATGGGTACATGACAG
ACCTGCAGCGTCCGCAGCCGCCCCCACCGCCGCCCGGTGCCCACAGCCCCGGAGCCACGCCCGGTCCCGG
GACCGCCACTGCCGAGAGGTCCTCCGGGGTCGCCCCAGCGGCCTCTCCGGCCGCCCCTAGCCCCGGGTCC
TCGGGGGGCGGTGGCTTCTTCTCGTCGCTGTCCAACGCGGTCAAGCAGACCACGGCGGCGGCAGCTGCCA
CCTTCAGCGAGCAGGTGGGCGGCGGCTCTGGGGGCGCAGGCCGCGGGGGAGCCGCCTCCAGGGTGCTGCT
GGTCATCGACGAGCCGCACACCGACTGGGCAAAATACTTCAAAGGGAAAAAGATCCATGGAGAAATTGAC
ATTAAAGTAGAACAGGCCGAATTCTCTGATCTCAACCTTGTGGCCCATGCCAATGGTGGATTCTCTGTGG
ATATGGAAGTTCTTCGGAATGGGGTGAAGGTCGTGCGGTCTCTGAAGCCGGATTTTGTGCTGATCCGCCA
GCACGCCTTCAGCATGGCACGCAACGGAGACTACCGCAGTTTGGTCATTGGGCTGCAGTATGCTGGAATC
CCCAGTGTTAACTCCTTGCATTCTGTCTACAACTTCTGTGACAAGCCCTGGGTGTTTGCCCAGATGGTTC
GACTGCATAAGAAACTGGGGACAGAAGAATTCCCTCTAATTGATCAGACCTTCTACCCCAATCACAAAGA
AATGCTCAGCAGTACAACGTACCCCGTGGTTGTGAAGATGGGGCACGCACACTCTGGGATGGGCAAGGTC
AAGGTTGACAACCAGCATGACTTCCAGGACATCGCAAGTGTCGTGGCACTGACCAAGACGTATGCCACTG
CCGAGCCCTTCATCGATGCCAAATATGACGTGCGTGTCCAGAAGATTGGGCAGAACTACAAGGCCTACAT
GAGGACGTCAGTGTCAGGGAACTGGAAGACCAATACTGGCTCTGCGATGCTGGAGCAAATTGCCATGTCT
GACAGATACAAGCTGTGGGTGGACACGTGCTCAGAGATTTTTGGGGGACTGGACATCTGCGCAGTGGAAG
CGCTACATGGCAAGGACGGAAGGGATCACATCATTGAGGTGGTGGGTTCCTCCATGCCGCTCATTGGTGA
CCACCAGGATGAAGACAAACAGCTCATCGTAGAGCTCGTGGTCAACAAGATGGCTCAGGCCCTGCCCCGG
CAGCGACAGCGGGATGCCTCCCCTGGCAGGGGCTCCCATGGCCAGACTCCGTCCCCAGGGGCCCTGCCCT
TGGGCCGCCAGACCTCCCAGCAGCCCGCAGGGCCCCCGGCTCAGCAGCGACCCCCACCACAGGGCGGCCC
TCCACAGCCGGGTCCAGGCCCCCAGCGCCAGGGACCCCCATTGCAGCAGCGCCCGCCCCCGCAGGGCCAG
CAGCACCTTTCAGGCCTTGGACCCCCAGCTGGCAGCCCCCTGCCCCAGCGCCTTCCAAGTCCCACCTCAG
CGCCCCAGCAGCCCGCGTCCCAGGCCGCGCCGCCGACCCAGGGTCAAGGCCGCCAATCCCGGCCAGTGGC
GGGAGGCCCCGGGGCGCCTCCAGCAGCCCGCCCGCCCGCCTCTCCGTCTCCCCAGCGCCAGGCGGGCCCC
CCACAGGCTACCCGTCAGACATCCGTCTCTGGCCCGGCTCCGCCAAAGGCCTCTGGGGCCCCACCGGGCG
GGCAGCAGCGCCAGGGCCCGCCCCAGAAACCCCCAGGCCCAGCCGGCCCCACACGCCAGGCCAGCCAGGC
GGGTCCCGTGCCCCGCACTGGGCCACCCACCACGCAGCAGCCTCGGCCCAGCGGCCCGGGCCCCGCTGGA
CGTCCCAAACCACAGCTGGCCCAGAAACCCAGCCAGGACGTGCCGCCACCCGCCACCGCCGCTGCAGGGG
GACCTCCGCACCCCCAGCTCAACAAATCCCAGTCTCTGACCAATGCCTTCAACCTTCCAGAGCCAGCCCC
GCCCAGGCCCAGCCTTAGCCAGGACGAGGTGAAAGCTGAGACCATCCGCAGCCTGAGGAAGTCTTTCGCC
AGCCTCTTCTCCGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221273 representing NM_006950
Red=Cloning site Green=Tags(s)

MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGS
SGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEID
IKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGI
PSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKV
KVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMS
DRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPR
QRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQ
QHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGP
PQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAG
RPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFA
SLFSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_006950
ORF Size 2115 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006950.3
RefSeq Size 2248 bp
RefSeq ORF 2118 bp
Locus ID 6853
UniProt ID P17600
Cytogenetics Xp11.3-p11.23
Domains Synapsin
MW 73.9 kDa
Summary This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC221273L1 Lenti ORF clone of Human synapsin I (SYN1), transcript variant Ia, Myc-DDK-tagged 10 ug
$946.00
RC221273L2 Lenti ORF clone of Human synapsin I (SYN1), transcript variant Ia, mGFP tagged 10 ug
$946.00
RC221273L3 Lenti ORF clone of Human synapsin I (SYN1), transcript variant Ia, Myc-DDK-tagged 10 ug
$946.00
RC221273L4 Lenti ORF clone of Human synapsin I (SYN1), transcript variant Ia, mGFP tagged 10 ug
$946.00
RG221273 SYN1 (tGFP-tagged) - Human synapsin I (SYN1), transcript variant Ia 10 ug
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SC314473 SYN1 (untagged)-Human synapsin I (SYN1), transcript variant Ia 10 ug
$646.00

Citations

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