Synapsin I (SYN1) (NM_006950) Human Tagged ORF Clone

SKU RG221273

SYN1 (tGFP-tagged) - Human synapsin I (SYN1), transcript variant Ia

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Synapsin I
• Synonyms: EPILX; MRX50; SYN1a; SYN1b; SYNI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221273 representing NM_006950; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAACTACCTGCGGCGCCGCCTGTCGGACAGCAACTTTATGGCCAATCTGCCAAATGGGTACATGACAG; ACCTGCAGCGTCCGCAGCCGCCCCCACCGCCGCCCGGTGCCCACAGCCCCGGAGCCACGCCCGGTCCCGG; GACCGCCACTGCCGAGAGGTCCTCCGGGGTCGCCCCAGCGGCCTCTCCGGCCGCCCCTAGCCCCGGGTCC; TCGGGGGGCGGTGGCTTCTTCTCGTCGCTGTCCAACGCGGTCAAGCAGACCACGGCGGCGGCAGCTGCCA; CCTTCAGCGAGCAGGTGGGCGGCGGCTCTGGGGGCGCAGGCCGCGGGGGAGCCGCCTCCAGGGTGCTGCT; GGTCATCGACGAGCCGCACACCGACTGGGCAAAATACTTCAAAGGGAAAAAGATCCATGGAGAAATTGAC; ATTAAAGTAGAACAGGCCGAATTCTCTGATCTCAACCTTGTGGCCCATGCCAATGGTGGATTCTCTGTGG; ATATGGAAGTTCTTCGGAATGGGGTGAAGGTCGTGCGGTCTCTGAAGCCGGATTTTGTGCTGATCCGCCA; GCACGCCTTCAGCATGGCACGCAACGGAGACTACCGCAGTTTGGTCATTGGGCTGCAGTATGCTGGAATC; CCCAGTGTTAACTCCTTGCATTCTGTCTACAACTTCTGTGACAAGCCCTGGGTGTTTGCCCAGATGGTTC; GACTGCATAAGAAACTGGGGACAGAAGAATTCCCTCTAATTGATCAGACCTTCTACCCCAATCACAAAGA; AATGCTCAGCAGTACAACGTACCCCGTGGTTGTGAAGATGGGGCACGCACACTCTGGGATGGGCAAGGTC; AAGGTTGACAACCAGCATGACTTCCAGGACATCGCAAGTGTCGTGGCACTGACCAAGACGTATGCCACTG; CCGAGCCCTTCATCGATGCCAAATATGACGTGCGTGTCCAGAAGATTGGGCAGAACTACAAGGCCTACAT; GAGGACGTCAGTGTCAGGGAACTGGAAGACCAATACTGGCTCTGCGATGCTGGAGCAAATTGCCATGTCT; GACAGATACAAGCTGTGGGTGGACACGTGCTCAGAGATTTTTGGGGGACTGGACATCTGCGCAGTGGAAG; CGCTACATGGCAAGGACGGAAGGGATCACATCATTGAGGTGGTGGGTTCCTCCATGCCGCTCATTGGTGA; CCACCAGGATGAAGACAAACAGCTCATCGTAGAGCTCGTGGTCAACAAGATGGCTCAGGCCCTGCCCCGG; CAGCGACAGCGGGATGCCTCCCCTGGCAGGGGCTCCCATGGCCAGACTCCGTCCCCAGGGGCCCTGCCCT; TGGGCCGCCAGACCTCCCAGCAGCCCGCAGGGCCCCCGGCTCAGCAGCGACCCCCACCACAGGGCGGCCC; TCCACAGCCGGGTCCAGGCCCCCAGCGCCAGGGACCCCCATTGCAGCAGCGCCCGCCCCCGCAGGGCCAG; CAGCACCTTTCAGGCCTTGGACCCCCAGCTGGCAGCCCCCTGCCCCAGCGCCTTCCAAGTCCCACCTCAG; CGCCCCAGCAGCCCGCGTCCCAGGCCGCGCCGCCGACCCAGGGTCAAGGCCGCCAATCCCGGCCAGTGGC; GGGAGGCCCCGGGGCGCCTCCAGCAGCCCGCCCGCCCGCCTCTCCGTCTCCCCAGCGCCAGGCGGGCCCC; CCACAGGCTACCCGTCAGACATCCGTCTCTGGCCCGGCTCCGCCAAAGGCCTCTGGGGCCCCACCGGGCG; GGCAGCAGCGCCAGGGCCCGCCCCAGAAACCCCCAGGCCCAGCCGGCCCCACACGCCAGGCCAGCCAGGC; GGGTCCCGTGCCCCGCACTGGGCCACCCACCACGCAGCAGCCTCGGCCCAGCGGCCCGGGCCCCGCTGGA; CGTCCCAAACCACAGCTGGCCCAGAAACCCAGCCAGGACGTGCCGCCACCCGCCACCGCCGCTGCAGGGG; GACCTCCGCACCCCCAGCTCAACAAATCCCAGTCTCTGACCAATGCCTTCAACCTTCCAGAGCCAGCCCC; GCCCAGGCCCAGCCTTAGCCAGGACGAGGTGAAAGCTGAGACCATCCGCAGCCTGAGGAAGTCTTTCGCC; AGCCTCTTCTCCGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221273 representing NM_006950; Red=Cloning site Green=Tags(s); ; MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGS; SGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEID; IKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGI; PSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKV; KVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMS; DRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPR; QRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQ; QHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGP; PQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAG; RPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFA; SLFSD; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006950
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006950.3
• RefSeq Size: 2248 bp
• RefSeq ORF: 2118 bp
• Locus ID: 6853
• UniProt ID: P17600
• Cytogenetics: Xp11.3-p11.23
• Domains: Synapsin
• Summary: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]