FGD4 (NM_139241) Human Tagged ORF Clone

SKU RC219442

FGD4 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 4 (FGD4)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FGD4
• Synonyms: CMT4H; FRABP; ZFYVE6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219442 representing NM_139241; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGTCTCAAAAGAAAAACCCAGTAAGGTATCAGATCTCA; TCAGTCGCTTTGAAGGAGGCAGCTCATTATCAAATTATAGTGATTTGAAGAAAGAGTCTGCTGTGAACCT; AAATGCTCCTAGAACCCCAGGAAGGCATGGATTGACAACCACACCTCAACAAAAACTCCTCTCCCAGCAC; TTGCCACAGAGGCAGGGAAATGATACAGATAAGACTCAGGGTGCACAGACTTGTGTGGCCAACGGTGTAA; TGGCAGCACAAAACCAGATGGAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTCAGATACTTCTATTCA; AGCTTCTGAACCCTTGCTTGATATGCACATAGTGAATGGAGAAAGAGATGAAACTGCCACAGCTCCTGCA; TCACCCACAACAGACAGCTGTGATGGAAATGCTTCTGACAGTAGCTACAGGACTCCAGGCATAGGCCCAG; TGCTCCCCCTAGAAGAAAGAGGGGCAGAAACAGAAACCAAGGTACAAGAGAGGGAAAATGGGGAAAGCCC; TCTGGAACTGGAGCAGCTGGACCAGCACCATGAGATGAAGGAGACTAATGAGCAAAAACTTCACAAAATA; GCCAATGAACTTTTGCTTACTGAAAGAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATT; GCAAACTGTTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATAT; TTCATCAATAAATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAA; ACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGAGAATATGTGA; AAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTGGT; TGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACTTTGCAGCATCACATGCTAGAACCTGTTCAG; CGGATTCCCCGGTATGAGATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGA; ATGATGCTAAAAAATCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAAT; GGAGAACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCA; AATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGAAACACTTCAGCACAAGAACGCTACC; TTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTGGTAGGCTCTAAATTCACAGT; TCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAGACTCAAAATGAAGAATATCCACATACTTTC; CAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCACAAGACAAAGAAGAATGGATCA; AGGCCCTTCAAGAAACCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGA; TAATGACATTCACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAAT; GAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCATCATTGTCGAG; CATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTTGAATATGATGGTGGTAAATT; GAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAAA; GGAATTTTAGAGATTGAATCAGCAGAAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGG; AGAAGTCAAAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTA; TGGTGCCCCCCAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCA; AGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTTGCTGCAGACA; GTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGTCC; AAATGAGCATCCAGCCACCTTGGATGATCATCCTGAACCTAAGAAAAAATCAGAATGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219442 representing NM_139241; Red=Cloning site Green=Tags(s); ; MEEIKPASASCVSKEKPSKVSDLISRFEGGSSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQH; LPQRQGNDTDKTQGAQTCVANGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDMHIVNGERDETATAPA; SPTTDSCDGNASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHHEMKETNEQKLHKI; ANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWE; TTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQ; RIPRYEMLLKDYLRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPS; NELIKEGQILKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTF; QVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHSEVSTAELGKRAPRWIRDN; EVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRK; GILEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMP; RSADLPHSFKLTQSKSVHSFAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139241
• ORF Size: 2298 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139241.3
• RefSeq Size: 2931 bp
• RefSeq ORF: 2301 bp
• Locus ID: 121512
• UniProt ID: Q96M96
• Cytogenetics: 12p11.21
• Domains: FYVE, PH, RhoGEF
• MW: 86.4 kDa
• Summary: This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]