FGD4 (NM_139241) Human Untagged Clone

SKU SC128121

FGD4 (untagged)-Human FYVE, RhoGEF and PH domain containing 4 (FGD4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FGD4
• Synonyms: CMT4H; FRABP; ZFYVE6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128121 sequence for NM_139241 edited (data generated by NextGen Sequencing); ATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGTCTCAAAAGAAAAACCCAGTAAGGTA; TCAGATCTCATCAGTCGCTTTGAAGGAGGCAGCTCATTATCAAATTATAGTGATTTGAAG; AAAGAGTCTGCTGTGAACCTAAATGCTCCTAGAACCCCAGGAAGGCATGGATTGACAACC; ACACCTCAACAAAAACTCCTCTCCCAGCACTTGCCACAGAGGCAGGGAAATGATACAGAT; AAGACTCAGGGTGCACAGACTTGTGTGGCCAACGGTGTAATGGCAGCACAAAACCAGATG; GAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTCAGATACTTCTATTCAAGCTTCTGAA; CCCTTGCTTGATATGCACATAGTGAATGGAGAAAGAGATGAAACTGCCACAGCTCCTGCA; TCACCCACAACAGACAGCTGTGATGGAAATGCTTCTGACAGTAGCTACAGGACTCCAGGC; ATAGGCCCAGTGCTCCCCCTAGAAGAAAGAGGGGCAGAAACAGAAACCAAGGTACAAGAG; AGGGAAAATGGGGAAAGCCCTCTGGAACTGGAGCAGCTGGACCAGCACCATGAGATGAAG; GAGACTAATGAGCAAAAACTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAAGAGCT; TATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTGTTGGAAGAAGCA; AACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATATTTCATCAATA; AATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAA; ACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGA; GAATATGTGAAAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATT; CCCCAGTTCAAATCAGTGGTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACT; TTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAGATGCTCCTTAAG; GACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGAATGATGCTAAAAAATCACTT; GAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGAACCTA; AAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCA; AATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGAAACACTTCAGCACAA; GAACGCTACCTTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTG; GTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAG; ACTCAAAATGAAGAATATCCACATACTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAA; CTGCAGGCCAGTTCTGCACAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAACCATC; GATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGATAATGACATT; CACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAAT; GAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCAT; CATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTT; GAATATGATGGTGGTAAATTGAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGA; TTCACAGACAGTGAAGAAAAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAGAAGTA; TCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCAAAACCTTGGCAG; AAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTATGGTGCCCCC; CAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCA; AGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTT; GCTGCAGACAGTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACA; GGTGAGACACCAGGTGGTCCAAATGAGCATCCAGCCACCTTGGATGATCATCCTGAACCT; AAGAAAAAATCAGAATGCTGA; ; Clone variation with respect to NM_139241.2; 374 c=>t;1305 g=>a;1518 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139241 unedited; GGGGTTCNCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGGGGA; GCCTGTGCTGGGTGAGATTATCTGCAATGGACCGGGTTAATGGGAGGCTTGCTTTGGCTC; GCTGGAAGAACAAATGAAGGGAGTTTTACTTCAACCATTTGTGACTTCTGGCCAAGAGGA; ATAAATTCAGCTTTTATAAGGCAGCAGTACCTGTCCAAAGATCGCTTTAGTTCCACCTTG; CTCCACAAGCAGCACAACCACACTGGTTGGTGAGAATGTATCTGAAGAAGAGGCTCAGGG; AATAAATGGGAACAGGCCAGCAAAACACTCAGCTGCAAGTCCAAAGCCACAAGTGCCTCC; AAAGCCATTACACCTGCAGAATTCACCTTCGTCCAATATACACCAAACCCCCAGGCATAA; AGCTTTACCTAGTGCAAAACCAAGGATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGT; CTCAAAAGAAAAACCCAGTAAGGTATCAGATCTCATCAGTCGCTTTGAAGGAGGCAGCTC; ATTATCAAATTATAGTGATTTGAAGAAAGAGTCTGCTGTGAACCTAAATGCTCCTAGAAC; CCCAGGAAGGCATGGATTGACAACCACACCTCAACAAAAACTCCTCTCCCAGCACTTGCC; ACAGAGGCAGGGAAATGATACAGATAAGACTCAGGGTGCACAGACTTGTGTGGCCAACGG; TGTAATGGCAGCACAAAACCAGATGGAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTC; AGAACTTCTATTCAAGCTTCTGAACCCTTGCTTGATATGCACATAGTGAATGGAGAAAGA; GATGAAACTGCCACAGCTCCTGCATCACCCACACAGACAGCTGTGATGGAAATGCTTCTG; ACAGTAGCTACAGGACTTCCAGCTAC;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_139241 unedited; GGGGCGGTTGTACATCTCAAAAAAGGTTTAATTTGNATAAAAAGCATAAGTTAAATAGTA; AACTTAGTGTGAGATTTTAATCACAAGTACAAAAGAGTGAAGAGCAGCCTTCATGACAAG; GAATCATGTGACCAGCCCCCACCCCAAACATAGGTTATCACATGTGGATTTTGTTTCATT; CCGACAAAGGAAAAGGCCCATACAGTTATCTTAAGTGTTCATTATATAGAGACCTTATTT; AACCATGCTCGATTATCTTACGGAAAAGAAAAACATGACGATAAATGTGGTTTCCACATA; TCCTAGTTCTGTAAAAAGTCAAGTGTCTCATAAAGTTTGGGTTTTGGTGAAAATGTTTGT; ACATGCTGATGTTAACTACTGCAATAATTATAGGATTGTTAACGGGTAAATAACCTGTAT; GACTTTGACTGTCCCACTGACCAATATGCACTTTAATCTTACAATAATCAAGACTGACGC; ATTAACTGAACCCTCATCTTATATTAATAGAACCTCTCTAGAGAGGCACTGTTTTAAATA; GGCCCTGACACAAAAAATGGGCCTTTAAAAGTACTGTTCGGAATTCACAAATTAGTGCCA; TGCCACATAAATTACCAATTTTTGTAAAAATATCAATCCCCTCCAATGCCAAATCGCAAA; ACCTAAATCACCCGAAACATTTCTTACATTTCAGAACATTGCTCAGTTCCCACGGTACAC; CTGGCTTTCATATTTAAAAATCAATTAGGGATTAATGTGGACCCTTACACCATGTCTAAC; ATATATATTTAACTCCACATAAACCAAATTGCTATTTCCTTAAGTACATTCTTAAGCAGT; CTTATTTGCAACACCATTATATTCCTTTCCTTTTTCAGTCGCGAAATACTAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_139241
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139241.1, NP_640334.1
• RefSeq Size: 2931 bp
• RefSeq ORF: 2301 bp
• Locus ID: 121512
• UniProt ID: Q96M96
• Cytogenetics: 12p11.21
• Domains: FYVE, PH, RhoGEF
• Summary: This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]; Transcript Variant: This variant (3) has an alternate 5' terminal exon, which results in a different 5' UTR and translation initiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.