TFIIE alpha (GTF2E1) (NM_005513) Human Tagged Lenti ORF Clone

SKU
RC218763L3
Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$757.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TFIIE alpha
Synonyms FE; TF2E1; TFIIE-A
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC218763).
Restriction Sites SgfI-MluI Cloning Scheme for this gene
ACCN NM_005513
ORF Size 1317 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005513.1, NP_005504.1
RefSeq Size 2969 bp
RefSeq ORF 1320 bp
Locus ID 2960
UniProt ID P29083
Cytogenetics 3q13.33
Domains TFIIE
Protein Families Transcription Factors
Protein Pathways Basal transcription factors
MW 49.3 kDa
Summary Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC218763 GTF2E1 (Myc-DDK-tagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1) 10 ug
$457.00
RC218763L1 Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), Myc-DDK-tagged 10 ug
$757.00
RC218763L2 Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mGFP tagged 10 ug
$757.00
RC218763L4 Lenti ORF clone of Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mGFP tagged 10 ug
$757.00
RG218763 GTF2E1 (tGFP-tagged) - Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1) 10 ug
$657.00
SC116700 GTF2E1 (untagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1) 10 ug
$457.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.